Plant/Fungi DNA Isolation Kits

Figure 2.  Purified DNA can be Amplified in a Real-time PCR Reaction (SYBR Green). DNA was isolated from 50 mg samples of plum and tobacco frozen leaves using Norgen’s Plant/Fungi DNA isolation Kit, and 2 µL of the eluted DNA was used in a real-time PCR reaction (total reaction volume 20 µL) with 18sr DNA primers (95°C for 3 minutes and 40 cycles at 95°C for 15 seconds and 60°C for 30 seconds). The real-time PCR was successful in amplifying both the plum and tobacco DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.

Figure 3. Resolution of DNA isolated from four different plant species. DNA was isolated from four different plant species using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Excellent DNA integrity and yield were observed from the Plant DNA Isolation Kit (Magnetic Bead System) (Lanes 1 to 3), indicating the robust performance comparable to the column based method (Red C). Marker = Norgen’s HighRanger DNA Ladder./p>

Figure 4. High DNA Quality. DNA was isolated from a number of plant species using Norgen's Plant DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). The purified DNA was then compared for DNA quality (A260/280). All DNA elutions isolated using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System) showed an excellent comparable DNA quality to Norgen’s Plant/Fungi DNA Isolation Kit (column based, Cat. 26200), indicating the consistent and robust performance of the Plant DNA Isolation Kit (Magnetic Bead System).

Figure 5. High DNA quality was confirmed by Real-time PCR. DNA was isolated from six different plant species using Norgen's Plan DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). To assess the quality of the purified DNA, 8 µL of the elution (total PCR reaction volume was 20 µL) was used in a real-time PCR reaction. As it can be seen, 18s rDNA amplification was successful in all cases without PCR inhibition, indicating the excellent quality of DNA purified using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System).

Figure 6. DNA was Isolated From Plant Tissue Using Norgen’s Plant DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Excellent DNA integrity, consistency, and yield were observed from the Plant DNA Isolation 96-Well Kit (Magnetic Bead System). Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900).

Figure 7. High DNA Quality Confirmed By Real-time PCR. DNA was isolated from plant tissue species using Norgen's Plant DNA Isolation 96-Well Kit (Magnetic Bead System). To assess the quality of the purified DNA, 2 µL of the elution (total PCR reaction volume was 20 µL) was used in a real-time PCR reaction. As it can be seen, 18s rDNA amplification was successful in all cases without PCR inhibition, indicating the excellent quality of DNA purified using Norgen’s Plant DNA Isolation 96-Well Kit (Magnetic Bead System).

Figure 8. Comparison of DNA extraction from plant tissue using the Plant DNA Isolation Kit (Magnetic Bead System) (Cat: 58200) on two automated platforms. Genomic DNA was extracted from 50 mg of plant tissue using the Isopure 96 and KingFisher automated systems. The gel image (right) shows 10 µL of extracted DNA run on a 1% agarose gel in 1X TAE buffer. A Norgen UltraRanger 1 kb DNA Ladder (Cat: 12100) was loaded in the first lane. High molecular weight DNA was successfully extracted from all samples with comparable band intensity between platforms. The bar graph (left) shows average Ct values from qPCR targeting the 18S rRNA gene, using 3 µL of eluate per 20 µL reaction. Both platforms yielded similar DNA quality, with Ct values of 12.60 (Isopure) and 12.82 (KingFisher), indicating efficient PCR amplification from both extraction methods.