For rapid and efficient endotoxin removal from proteins and peptides

  • Rapid and convenient spin column format
  • Columns bind proteins of interest while endotoxins flow through
  • Reduce endotoxin levels to less than 0.1 EU/µg of protein
  • Greater than 95% protein recovery
  • High protein recovery with excellent endotoxin reduction

ItemsCat. #Size
ProteoSpin™ Endotoxin Removal Maxi Kit - For Proteins and Peptides 22200 4 preps

ProteoSpin™ Endotoxin Removal Maxi Kit

This kit rapidly removes endotoxins from up to 4 mg of previously purified protein or peptides. The ProteoSpin™ Endotoxin Removal Maxi Kit efficiently reduces endotoxin levels to ≤ 0.1 EU/µg of protein using convenient spin columns.

The kit contains sufficient materials for 4 purifications. Each column is able to remove endotoxins from up to 4 mg of previously purified proteins, with protein recoveries of > 95% being achieved. Preparation time for a single sample is approximately 30 minutes. The purified protein samples can then be used in a number of downstream applications including in vitro and in vivo introduction into cells and organisms, Western Blots, Mass Spectrometry and more.

About Endotoxins

Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxinsin vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications including introduction into cells and organisms for example.

Kit Specifications
Maximum Input Volume
4 mg
Maximum Initial Protein Volume Input
18 mL
Final Endotoxin Levels
<= 0.01 EU/μg protein
Average Recovery
Time to Complete 10 Purifications
30 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 2 years in their unopened containers.

Kit Components
Component Cat. 22200 (4 preps)
Binding Buffer J 8 mL
Wash Solution M 130 mL
Elution Buffer G 20 mL
Endotoxin Removal Solution 1.5 mL
Protein Neutralizer EF 2 mL
Maxi Spin Columns (assembled with Collection Tubes) 4
Elution Tubes (50 mL) 4
Product Insert 1

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