For rapid and efficient endotoxin removal from proteins and peptides

  • Rapid and convenient spin column format
  • Columns bind proteins of interest while endotoxins flow-through
  • Reduce endotoxin levels to less than 0.1 EU/µg of protein
  • Greater than 95% protein recovery
  • High protein recovery with excellent endotoxin reduction

Items Cat. # Size
ProteoSpin Endotoxin Removal Maxi Kit - For Proteins and Peptides 22200 4 preps

ProteoSpin Endotoxin Removal Maxi Kit

This kit rapidly removes endotoxins from up to 4 mg of previously purified protein or peptides.  The ProteoSpin™ Endotoxin Removal Maxi Kit efficiently reduces endotoxin levels to ≤ 0.1 EU/µg of protein using convenient spin columns. 

The kit contains sufficient materials for 4 purifications. Each column is able to remove endotoxins from up to 4 mg of previously purified proteins, with protein recoveries of > 95% being achieved. Preparation time for a single sample is approximately 30 minutes.  The purified protein samples can then be used in a number of downstream applications including in vitro and in vivo introduction into cells and organisms, Western Blots, Mass Spectrometry and more...

 

About Endotoxins

Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses.  Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications including introduction into cells and organisms for example

Kit Specifications
Maximum Input Volume
4 mg
Maximum Initial Protein Volume Input
18 mL
Final Endotoxin Levels
<= 0.01 EU/μg protein
Average Recovery
>95%
Time to Complete 10 Purifications
30 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 2 years in their unopened containers.


Kit Components
Component Cat. 22200 (4 preps)
Binding Buffer J 8 mL
Wash Solution M 130 mL
Elution Buffer G 20 mL
Endotoxin Removal Solution 1.5 mL
Protein Neutralizer EF 2 mL
Maxi Spin Columns (assembled with Collection Tubes) 4
Elution Tubes (50 mL) 4
Product Insert 1

Title A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB.
Journal BMC Veterinary Research. 2015.
Authors Dan Xiong, Li Song, Xianyue Zhai, Shizhong Geng, Zhiming Pan and Xinan Jiao.
Title Amino acids 89–96 of Salmonella typhimurium flagellin represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response.
Journal Cellular & Molecular Immunology. 2014.
Authors Lei Zhang, Zhiming Pan, Xilong Kang, Yun Yang, Heekap Kang, Na Zhang, James M Rosati and Xinan Jiao.
Title Pulmonary CD103+ dendritic cells prime Th2 responses to inhaled allergens.
Journal Mucosal Immunology. 2011.
Authors Nakano H, Free M, Whitehead G, Maruoka S, Wilson R, Nakano K and Cook D.
Title A Novel Core Genome-Encoded Superantigen Contributes to Lethality of Community-Associated MRSA Necrotizing Pneumonia.
Journal PLoS Pathogens. 2011.
Authors Wilson G, Seo KS, Cartwright R, Connelley T, Chuang-Smith O, Merriman J, Guinane C, Park J, Bohach G, Schlievert P, Morrison W and Fitzgerald J.
Title Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli.
Journal Peptides. 2010.
Authors Bommarius B, Jenssen H, Elliott M, Kindrachuk J, Pasupuleti M, Gieren H, Jaeger KE, Hancock RE, Kalman D.
Title An aberrant prostate antigen?specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans
Journal Journal of Clinical Investigation. 2009.
Authors Hou Y, DeVoss J, Dao V, Kwek S, Simko JP, McNeel DG, Anderson MS, Fong L.
Title Development and Characterization of a TAPIR-Like Mouse Monoclonal Antibody to Amyloid-beta
Journal Journal of Alzheimer's Disease.Vol 4, no 2, 161-173. 2008.
Authors Wang J, Hara H, Makifuchi T, Tabira T.
Title Immunotherapy of hepatocellular carcinoma with a vaccine based on xenogeneic homologous a fetoprotein in mice
Journal Biochemical and Biophysical Research Communications. 2008.
Authors Zhang W, Liu J, Wu Y, Xiao F, Wang Y, Wang R, Yang H, Wang G, Yang J, Deng H, Li J, Wen Y, Wei Y.