For rapid and efficient endotoxin removal from proteins and peptides
|ProteoSpin™ Endotoxin Removal Maxi Kit - For Proteins and Peptides||22200||4 preps|
ProteoSpin™ Endotoxin Removal Maxi Kit
This kit rapidly removes endotoxins from up to 4 mg of previously purified protein or peptides. The ProteoSpin™ Endotoxin Removal Maxi Kit efficiently reduces endotoxin levels to ≤ 0.1 EU/µg of protein using convenient spin columns.
The kit contains sufficient materials for 4 purifications. Each column is able to remove endotoxins from up to 4 mg of previously purified proteins, with protein recoveries of > 95% being achieved. Preparation time for a single sample is approximately 30 minutes. The purified protein samples can then be used in a number of downstream applications including in vitro and in vivo introduction into cells and organisms, Western Blots, Mass Spectrometry and more.
Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications including introduction into cells and organisms for example.
|Maximum Input Volume||
|Maximum Initial Protein Volume Input||
|Final Endotoxin Levels||
<= 0.01 EU/μg protein
|Time to Complete 10 Purifications||
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 2 years in their unopened containers.
|Title||A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB.|
|Journal||BMC Veterinary Research. 2015.|
|Authors||Dan Xiong, Li Song, Xianyue Zhai, Shizhong Geng, Zhiming Pan and Xinan Jiao.|
|Title||Amino acids 89–96 of Salmonella typhimurium flagellin represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response.|
|Journal||Cellular & Molecular Immunology. 2014.|
|Authors||Lei Zhang, Zhiming Pan, Xilong Kang, Yun Yang, Heekap Kang, Na Zhang, James M Rosati and Xinan Jiao.|
|Title||Pulmonary CD103+ dendritic cells prime Th2 responses to inhaled allergens.|
|Journal||Mucosal Immunology. 2011.|
|Authors||Nakano H, Free M, Whitehead G, Maruoka S, Wilson R, Nakano K and Cook D.|
|Title||A Novel Core Genome-Encoded Superantigen Contributes to Lethality of Community-Associated MRSA Necrotizing Pneumonia.|
|Journal||PLoS Pathogens. 2011.|
|Authors||Wilson G, Seo KS, Cartwright R, Connelley T, Chuang-Smith O, Merriman J, Guinane C, Park J, Bohach G, Schlievert P, Morrison W and Fitzgerald J.|
|Title||Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli.|
|Authors||Bommarius B, Jenssen H, Elliott M, Kindrachuk J, Pasupuleti M, Gieren H, Jaeger KE, Hancock RE, Kalman D.|
|Title||An aberrant prostate antigen?specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans|
|Journal||Journal of Clinical Investigation. 2009.|
|Authors||Hou Y, DeVoss J, Dao V, Kwek S, Simko JP, McNeel DG, Anderson MS, Fong L.|
|Title||Development and Characterization of a TAPIR-Like Mouse Monoclonal Antibody to Amyloid-beta|
|Journal||Journal of Alzheimer's Disease.Vol 4, no 2, 161-173. 2008.|
|Authors||Wang J, Hara H, Makifuchi T, Tabira T.|
|Title||Immunotherapy of hepatocellular carcinoma with a vaccine based on xenogeneic homologous a fetoprotein in mice|
|Journal||Biochemical and Biophysical Research Communications. 2008.|
|Authors||Zhang W, Liu J, Wu Y, Xiao F, Wang Y, Wang R, Yang H, Wang G, Yang J, Deng H, Li J, Wen Y, Wei Y.|
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