For efficient lysis of red blood cells

  • Rapid and differential lysis of red blood cells
  • Faciliates removal of abundant proteins and RNAs present in red blood cells
  • Resulting leukocyte pellets can be used for subsequent RNA, DNA or protein purification

ItemsCat. #Size
RBC Lysis Buffer 21201 90 mL
RBC Lysis Buffer 21205 500 mL
RBC Lysis Solution 21207 1000 mL

RBC Lysis Buffer

Norgen’s RBC Lysis Buffer is designed for the differential lysis of red blood cells present in whole blood samples. The removal of red blood cells from whole blood is often desirable, particularly during the study of leukocyte DNA, proteins or RNA. Abundant proteins (including albumin) and RNAs (including globin mRNA) that are present in red blood cells and may interfere with downstream applications such as expression analysis are effectively removed during this process. For the procedure, whole blood samples are first collected in the presence of anticoagulants. The red blood cells are then removed through lysis using the RBC Lysis Buffer, and the leukocytes which remain can then be recovered by centrifugation. Genomic DNA, proteins or RNA can then be isolated from the purified leukocytes, as Norgen’s RBC Lysis Buffer is RNAase and DNase free.

Available Sizes

  • 90 mL
  • 500 mL
  • 1000 mL

Available in bottles of 90 mL, 500 mL and 1000 mL.

Product insert included.

Storage Conditions and Product Stability
The RBC Lysis Buffer should be stored at 4°C. This reagent should remain stable for at least 1 year in its unopened container.

 

 

Title Antenatal Suppression of IL-1 Protects against Inflammation-Induced Fetal Injury and Improves Neonatal and Developmental Outcomes in Mice
Journal Journal of Immunology. 2017.
Authors Nadeau-Vallée M, Chin PY, Belarbi L, Brien MÈ, Pundir S, Berryer MH, Beaudry-Richard A, Madaan A, Sharkey DJ, Lupien-Meilleur A, Hou X, Quiniou C, Beaulac A, Boufaied I, Boudreault A, Carbonaro A, Doan ND, Joyal JS, Lubell WD, Olson DM, Robertson SA, Girard S, Chemtob S
Title Social threat exposure in juvenile mice promotes cocaine-seeking by altering blood clotting and brain vasculature
Journal Addiction Biology. 2016.
Authors Lo Iacono L, Valzania A, Visco-Comandini F, Aricò E, Viscomi MT, Castiello L, Oddi D, D'Amato FR, Bisicchia E, Ermakova O, Puglisi-Allegra S, Carola V