RNA/DNA/Protein Purification Plus Kits For sequential isolation of total RNA, genomic DNA and total proteins from the same sample For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA/DNA/Protein Purification Plus Kits For sequential isolation of total RNA, genomic DNA and total proteins from the same sample Price USD$819.00 SKU 47700 Format Plus Micro Plus High Throughput Plus Size 50 Preps Quantity Request sample Overview Details Documentation Citations Overview Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample No sample splitting or need to use phenol or precipitation methods Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix These kits are ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA/Protein Purification Plus Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications. RNA/DNA/Protein Purification Plus Kit This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen's resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. RNA/DNA/Protein Purification Plus Micro Kit This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits. RNA/DNA/Protein Purification 96-Well Plus Kit The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen's resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below. Details Supporting Data Previous Figure 1. Recovery of True Total RNA including microRNA from HEK-293 Cells. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of ~ 800,000 HEK-293 cells using either Norgen's RNA/DNA/Protein Purification Plus Kit or a competitor's multiple-analyte purification kit. Both kits isolated large RNA (represented by 28S and 18S rRNA) with high integrity but Norgen's RNA/DNA/Protein Purification Plus Kit provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA/Protein Purification Plus Kit showed the added benefit of recovering small RNA. The difference in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for human S15 (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen's RNA/DNA/Protein Purification Plus Kit showed similar Ct value to RNA isolated by the competitor's kit for the large RNA (Panel D). However, Norgen's RNA/DNA/Protein Purification Plus Kit showed superior recovery of small RNA including microRNAs as depicted by the miR-21 RT-qPCR (Panel C). Figure 2. Recovery of True Total RNA including microRNA from Hamster Liver. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of 15 mg hamster liver using either Norgen's RNA/DNA/Protein Purification Plus Kit or a competitor's multiple-analyte purification kit. Both kits isolated large RNA (represented by 28S and 18S rRNA) with high integrity but Norgen's RNA/DNA/Protein Purification Plus Kit provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA/Protein Purification Plus Kit showed the added benefit of recovering small RNA. The difference in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for hamster beta-Actin (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen's RNA/DNA/Protein Purification Plus Kit showed similar Ct value to RNA isolated by the competitor's kit for the large RNA (Panel D). However, Norgen's RNA/DNA/Protein Purification Plus Kit showed superior recovery of small RNA including microRNAs as depicted by the miR-21 RT-qPCR (Panel C). Figure 3. Recovery of Intact, High Quality Genomic DNA from HEK-293 cells and Hamster Liver. Panel A is a 1% agarose gel showing the gDNA isolated from the same HEK-293 cell or hamster liver samples using Norgen's RNA/DNA/Protein Purification Plus Kit or competitor's multiple-analyte purification kit. Lane M is Norgen's HighRanger 1 kb DNA Ladder and the sample lanes contain 10 µL of each of the 100 µL elutions. The gel showed high quality, and intact genomic DNA, with a better yield using Norgen's RNA/DNA/Protein Purification Plus Kit. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgen's RNA/DNA/Protein Purification Plus Kit is of high quality and performed similar to or better than competitor's product. Figure 4. High Quality Total Proteins Eluted in Mass Spec-Compatible Buffer. Norgen's RNA/DNA/Protein Purification Plus Kit provides an additional column purification step for effective concentration and clean-up of the isolated proteins. In contrast, most competing multiple analyte isolation products require protein precipitation and the precipitated proteins are required to be resuspended in buffer with high-detergent content (such as SDS-PAGE loading dye) for full recovery. Protein fractions (from hamster liver) isolated by Norgen's RNA/DNA/Protein Purification Plus Kit and a competitor's kit were resolved on a 12% SDS-PAGE protein gel. Panel A showed that when the competitor's precipitated protein fraction was resuspended in a provided SDS-PAGE loading buffer, the protein recovery was similar among the two kits. Panel B showed that when the same precipitated protein fraction from the competitor's kit was resuspended in a Tris-based buffer containing no detergent or denaturant, the protein recovery became drastically reduced. In contrary, Norgen's RNA/DNA/Protein Purification Plus Kit purified proteins by column and the eluted proteins are already in a buffer compatible with most downstream applications including mass spectrophotometry as well as standard protein quantification methods (including Bradford assays). Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg for RNA 20 μg for DNA 200 μg for protein Maximum Column Loading Volume 650 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Size of DNA Purified ≥ 30 kb Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues 5 x 106 cells 25 mg (for selected tissues) 100 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg Time to Complete 10 Purifications 30 minutes Average Yield: HeLa Cells (1 x 106 cells) HeLa Cells (1 x 106 cells) HeLa Cells (1 x 106 cells) 15 μg RNA 8 μg DNA 150 μg protein Storage Conditions and Product Stability The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 47700 (50 preps) Cat. 51600 (50 preps) Cat. 51700 (96 preps) Buffer SKP 40 mL 40 mL - Lysis Buffer Q - - 40 mL Wash Solution A 2 x 38 mL1 x 18 mL 2 x 38 mL1 x 18 mL 2 x 38 mL1 x 18 mL Elution Solution A 6 mL 6 mL 20 mL Elution Buffer F 15 mL 15 mL 2 x 15 mL Wash Solution C 30 mL 30 mL 60 mL Binding Buffer A 8 mL 8 mL 8 mL Elution Buffer C 8 mL 8 mL 30 mL Protein Neutralizer 4 mL 4 mL 4 mL Protein Loading Dye 2 mL 2 mL 3 x 2 mL gDNA Purification Columns 50 - - gDNA Purification Micro Columns - 50 - gDNA Purification 96-Well Plate - - 1 RNA/Protein Purification Columns 50 - - RNA/Protein Purification Micro Columns - 50 - RNA/Protein Purification 96-Well Plate - - 1 Collection Tubes 150 150 - Collection Plate - - 5 Elution Tubes (1.7 mL) 150 150 - Elution Plate - - 3 Lysis Preparation Plate - - 2 Adhesive Tape - - 4 Product Insert 1 1 1 Documentation PROTOCOLS (47700) RNA/DNA/Protein Purification Plus Kit - Protocol (50 prep) (51600) RNA/DNA/Protein Purification Plus Micro Kit - Protocol (50 prep) (51700) RNA/DNA/Protein Purification 96-Well Plus Kit - Protocol (96-well) APPLICATION NOTES Sequential Isolation and Purification of RNA, DNA and Protein from a Single Sample of Animal Cells using Norgen’s RNA/DNA/Protein Purification Kit Sequential Purification of RNA, DNA and Protein from a Single Sample using Norgen's RNA/DNA/Protein Purification Kit and Comparison to a Market Competitor Supplementary Protocol for Total RNA Isolation from Staphylococcus aureus-Related Bacteria and Hard-to-Lyse Bacteria Species SAFETY DATA SHEETS (47700) RNA/DNA/Protein Purification Plus Kit - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products RNase-Free DNase I Kit
RNA/DNA/Protein Purification Plus Kits For sequential isolation of total RNA, genomic DNA and total proteins from the same sample For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA/DNA/Protein Purification Plus Kits For sequential isolation of total RNA, genomic DNA and total proteins from the same sample Price USD$819.00 SKU 47700 Format Plus Micro Plus High Throughput Plus Size 50 Preps Quantity Request sample