For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Figure 1. TaqMan PCR Kit.
Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.
Figure 2. TaqMan PCR Kit.
Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR..
Figure 3. TaqMan PCR Kit.
Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.
Figure 4. Lyophilized qPCR kits produced virtually identical results to non-lyophilized kits in terms of Ct values.
Storage Conditions and Product Stability
All kit components should be stored between -20°C for 2 years after the date of production without showing any reduction in performance, irrespective of when the kit components are reconstituted.
| Component | Cat. TM33350L (100 preps) | Cat. TM3330L (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix (Lyo) | 4 x 350 μL | - |
| Saccharomyces cerevisiae Primer & Probe Mix (Lyo) | 280 μL | 280 μL |
| Saccharomyces cerevisiae Positive Control (Lyo) | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 3 x 1.25 mL | 1 x 1.25 mL |
| Product Insert | 1 | 1 |
Listed volumes of components marked with (Lyo) are the volumes upon reconstitution
