Intended for in vitro diagnostics use.
Research version available here
This product is not available for purchase in the United States.
Details
Supporting Data
Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for up to 75 Months.
Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 75 months. Saliva DNA was subsequently isolated at 1 week, 3 weeks, 1 month, 2 months, 4 months, 6 months, 8 months, 12 months, 20 months, 32 months, 40 months, 52 months and 75 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 75 months at ambient temperatures in Norgen's Saliva DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 75-month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).
Figure 2. GAPDH Real Time PCR Amplification of Saliva DNA from Saliva Samples Stored at Room Temperature from Day 0 up to 24 Months.
All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition and difference in Ct. Red: Day 0, Blue: 4 months, Purple: 8 months, Green: 16 months and Sky blue: 24 months. Black: No template control.
Figure 3. GAPDH Real Time PCR Amplification of Saliva DNA from Saliva Samples Stored at Room Temperature from Day 0 up to 24 Months.
All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition and difference in Ct. Red: Day 0, Blue: 4 months, Purple: 8 months, Green: 16 months and Sky blue: 24 months. Black: No template control.
Figure 4. V3-V4 16s rRNA PCR amplification for Illumina MiSeq 16s rRNA library preparation.
The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 2 years at room temperature in Norgen’s Saliva DNA Preservative. The PCR result shows the clean PCR amplification at ~550 bp indicating the right target of V3-V4 16s rRNA , with no difference between day 0 and 24 months. M: FastRunner DNA Ladder (Cat. 12800).
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Kit Specifications
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Volume of Saliva Collected
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2 mL
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Volume of Saliva-Preservative Mix
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4 mL
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Preservation Temperature
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Room Temperature
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| Preservation time |
Up to 6 years at room temperature
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Shelf Life and Handling
- The Collection Device should be kept tightly sealed and stored at room temperature up to the Collect Before Date (indicated on the device label) without any reduction in kit performance.
- Once collected, saliva is stable for up to 6 years when kept tightly sealed and stored at room temperature.
- The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.
| Kit Components | Cat. 49000 (50 Devices) |
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| Individual Saliva DNA Collection and Preservation Devices | 50 |
| Donor Procedure Flowchart | 1 |
| Product Insert | 1 |
| Individual Saliva DNA Collection and Preservation Device Contents | |
| Saliva Collection Funnel and Collection Tube | 1 |
| Collection Tube Cap | 1 |
| Preservative Ampoule | 1 |
| Donor Instructions | 1 |
