A user-friendly all-in-one procedure for the collection, preservation and isolation of saliva DNA at ambient temperatures

  • Reliable and cost-effective
  • Non-invasive, user-friendly sample collection
  • Sample collection, preservation and DNA isolation in one convenient kit
  • Samples are non-infectious and can be handled and shipped safely
  • Preserved DNA is stable for years at ambient temperature
  • Compatible with most DNA isolation methods
  • High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
  • Shipping accessories can be purchased separately

ItemsCat. #Size
Saliva Collection, Preservation and Isolation Kit RU35700 50 Devices

Saliva DNA Collection, Preservation and Isolation Kit

Norgen’s Saliva DNA Collection, Preservation and Isolation Kit is an all-in-one solution designed for:

  1. Simple and non-invasive saliva collection;
  2. Preservation of DNA in saliva samples at ambient temperature;
  3. Isolation of high quality DNA within a laboratory setting.

The Saliva DNA Collection, Preservation and Isolation Kit contains 50 Individual Saliva DNA Collection and Preservation Devices, as well as the required reagents for the subsequent laboratory isolation of the saliva DNA from the preserved samples. Each of the 50 Individualized Saliva DNA Collection, Preservation and Isolation Kits consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen’s Saliva DNA Preservative contained within a sealed squeezable ampoule. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 5 years at room temperature. This kit is ideal for collecting, preserving and isolating DNA samples for epidemiological and population studies.

Saliva DNA from Preservative

Norgen’s Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 5 years without any detectable DNA degradation.

Nucleic Acid Isolation from Preservative

Saliva DNA is isolated from the preserved saliva samples via alcohol precipitation using the provided reagents. Saliva DNA can also be isolated from the preserved saliva samples using Norgen's spin column-based kit (Norgen's Saliva DNA Isolation Kit - Cat# RU45400) or Norgen's 96-well format kit (Norgen's Saliva DNA Isolation 96-Well Kit - Cat# RU35200).

Kit Specifications
Volume of Saliva Collected
2 mL
Volume of Saliva-Preservative Mix
4 mL
Preservation Temperature

Room temperature

Preservation Time
Up to 75 months at room temperature
Time to Complete DNA Isolation
35 minutes
Average Yield from 0.5 mL*
5 - 20 μg
Average OD 260/280
1.7

* Average DNA yield will vary depending on the health status of the donor

Shelf Life and Handling
The Collection Device should be kept tightly sealed and stored at room temperature for up to 5 years without any reduction in kit performance (The collection due date is written on the device label). The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at -20°C up to the expiry date indicated on the label (with minimum freeze and thaw). Store all other kit components at room temperature. The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.


Kit Components
Component Cat. RU35700 (50 devices)
Individual Saliva DNA Collection and Preservation Devices 50
Proteinase K 1.2 mL
Binding Buffer B 12 mL
Product Insert 1
Individual Saliva DNA Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube 1
Collection Tube Cap 1
Preservative Ampoule 1
Donor Instructions 1

Product Information Sheets: 
Application Notes: 

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Title Detection of Novel Integrons in the Metagenome of Human Saliva
Journal PLOS ONE. 2016.
Authors Tansirichaiya S, Rahman MA, Antepowicz A, Mullany P, Roberts AP
Title Increased risk of attention-deficit/hyperactivity disorder associated with exposure to organophosphate pesticide in Taiwanese children
Journal Andrology. 2016.
Authors Yu CJ, Du JC, Chiou HC, Chung MY, Yang W, Chen YS, Fuh MR, Chien LC, Hwang B, Chen ML
Title Attention Deficit/Hyperactivity Disorder and Urinary Nonylphenol Levels: A Case-Control Study in Taiwanese Children
Journal PLOS ONE. 2016.
Authors Yu CJ, Du JC, Chiou HC, Yang SH, Liao KW, Yang W, Chung MY, Chien LC, Hwang B, Chen ML
Title Functional polymorphisms in the P2X7 receptor gene are associated with stress fracture injury
Journal Purinergic Signalling. 2016.
Authors Varley I, Greeves JP, Sale C, Friedman E, Moran DS, Yanovich R, Wilson PJ, Gartland A, Hughes DC, Stellingwerff T, Ranson C, Fraser WD, Gallagher JA
Title Selective familiarity deficits in otherwise cognitively intact aging individuals with genetic risk for Alzheimer's disease
Journal Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2015.
Authors Dorothee Schoemaker, Judes Poirier, Sophia Escobar, Serge Gauthier, Jens Pruessner
Title Is less really more: Does a prefrontal efficiency genotype actually confer better performance when working memory becomes difficult?
Journal Cortex. 2015.
Authors Jessica L. Ihne, Natalie M. Gallagher, Marie Sullivan, Joseph H. Callicott, Adam E. Green
Title A Preliminary Genome-Wide Association Study of Acute Mountain Sickness Susceptibility in a Group of Nepalese Pilgrims Ascending to 4380 m
Journal High Altitude Medicine & Biology. 2015.
Authors MacInnis MJ, Widmer N, Timulsina U, Subedi A, Siwakoti A, Pandit BP, Freeman MG, Carter EA, Manokhina I, Thapa GB, Koehle MS
Title Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression
Journal BMC Pregnancy and Childbirth. 2015.
Authors Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen
Title The dentin phosphoprotein repeat region and inherited defects of dentin
Journal Molecular Genetics & Genomic Medicine. 2015.
Authors Jie Yang, Kazuhiko Kawasaki, Moses Lee, Bryan M. Reid, Stephanie M. Nunez, Murim Choi, Figen Seymen, Mine Koruyucu, Yelda Kasimoglu, Ninna Estrella-Yuson, Brent P. J. Lin, James P. Simmer and Jan C.-C. Hu
Title Taurodontism, variations in tooth number, and misshapened crowns in Wnt10a null mice and human kindreds.
Journal Molecular Genetics & Genomic Medicine. 2014.
Authors Jie Yang, Shih-Kai Wang, Murim Choi, Bryan M. Reid, Yuanyuan Hu, Yuan-Ling Lee, Curtis R. Herzog, Hera Kim-Berman, Moses Lee, Paul J. Benke, K. C. Kent Lloyd, James P. Simmer and Jan C.-C. Hu.
Title Patterns of Admixture and Population Structure in Native Populations of Northwest North America.
Journal PLOS Genetics. 2014.
Authors Paul Verdu, Trevor J. Pemberton, Romain Laurent, Brian M. Kemp, Angelica Gonzalez-Oliver, Clara Gorodezky, Cris E. Hughes, Milena R. Shattuck,Barbara Petzelt, Joycelynn Mitchell, Harold Harry, Theresa William, Rosita Worl, Jerome S. Cybulski, Noah A. Rosenberg, Ripan S. Malhi.
Title Association of premenstrual/menstrual symptoms with perinatal depression and a polymorphic repeat in the polyglutamine tract of the retinoic acid induced 1 gene
Journal Journal of Affective Disorders. 2014.
Authors Ene-Choo Tan, Hui-San Tan, Tze-Ern Chua, Theresa Lee, Jasmine Ng, Ying-Chia Ch’ng, Chih-Huei Choo, Helen Y Chen
Title RANK/RANKL/OPG pathway: Genetic associations with stress fracture period prevalence in elite athletes.
Journal The Bone Journal. 2014.
Authors Ian Varley, David C. Hughes, Julie P. Greeves, Trent Stellingwerff, Craig Ranson, William D. Fraser, Craig Sale.