• Recombinant source
  • Functionality preserved at wide range of temperatures
  • Highly reliable and reproducible reactions
  • Supplied with detergent-free Reaction Buffer for compatibility with microarray and DHPLC applications
  • Compatible with the use of modified nucleotides (dUTP, dITP, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Available in 2 different concentrations: 5 units/µL and 1 unit/µL

Items Cat. # Size
Taq DNA Polymerase with 10X Taq Reaction Buffer 28088 1000 Units
Taq DNA Polymerase with 10X Taq Reaction Buffer 28094 200 units (1 U/uL)

Taq DNA Polymerase with 10X Taq Reaction Buffer

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity.  The enzyme is supplied with detergent-free 10X Taq Reaction Buffer, making it compatible with microarray and DHPLC applications.

Norgen’s Taq DNA Polymerase with 10X Taq Reaction Buffer is available in 2 convenient sizes:
 - 1000 units at a concentration of 5000 units/mL (Cat #28088)
 - 200 units at a concentration of 1000 units/mL (Cat #28094)

Enzyme Source:
An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1

  • Routine PCR
  • Preparation of PCR products for cloning
  • Primer extension
  • Microarray analysis
  • DHPLC
  • DNA labeling
  • DNA sequencing

Reagents supplied - Cat# 28088: 

  • 1000 units of Taq DNA Polymerase (5000 units/mL)
  • 4 x 1.25 mL Taq Reaction Buffer (10X)

Reagents supplied - Cat# 28094:

  • 200 units of Taq DNA Polymerase (1000 units/mL)
  • 1 x 1.25 mL Taq Reaction Buffer (10X)

Reaction Conditions:
1X Taq Reaction Buffer (10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, PCR stabilizer, pH 8.3 @ 25°C)

Storage Buffer:
50 mM Tris-HCl pH 8 at 25°C, 50 mM KCl, 50mM NaCl, 0.5 mM Dithiothreitol, 0.1 mM EDTA, 50% Glycerol, 1% Triton X-100

Storage Temperature:
-20°C

Title Association of the genetic variants of insulin receptor substrate 1 (IRS-1) with type 2 diabetes mellitus in a Saudi population.
Journal PLoS One. 2014.
Authors Alharbi KK, Khan IA, Munshi A, Alharbi FK, Al-Sheikh Y, Alnbaheen MS.
Title Crp is a global regulator of antibiotic production in streptomyces.
Journal mBio. 2012.
Authors Gao C, Hindra, Mulder D, Yin C, Elliot MA.