For research use only and NOT intended for in vitro diagnostics.
A ready-to-use system for the isolation and detection of XMRV viral RNA from urine using end-point RT-PCR
|Urine-Based XMRV RT-PCR Detection Kit||34700||24 tests|
|Urine-Based XMRV RT-PCR Primers and Controls||34710||100 Rxns|
Urine-Based XMRV RT-PCR Detection Kit
Xenotropic murine leukemia virus-related virus (XMRV) belongs to the family Retroviridae and the genus gammaretrovirus. The virus was first described in 2006 and has since been isolated from human biological samples. Norgen’s Urine-Based XMRV RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of XMRV from urine. First the kit contains components for the rapid isolation of total RNA, including viral RNA, from the urine samples using spin-column chromatography based on Norgen’s proprietary resin. Second the kit contains XMRV RT-PCR Master Mix and controls for PCR Amplification, as well as a Control RT-PCR Master Mix to allow for amplification of both an Isolation Control and a PCR Control. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed by real-time PCR using melt curves.
The XMRV Master Mix contains reagents and enzymes for the specific amplification of a 300 bp region of XMRV. In addition, Norgen’s Urine-Based XMRV RT-PCR Detection Kit contains a second Master Mix, the Control 2x RT-PCrR Master Mix, which can be used to identify possible PCR inhibition and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples. The XMRV RT-PCR Primer Set and Controls are also available separately for end-point RT-PCR detection.
• The linear range of Norgen’s XMRV RT-PCR Kit Dx was determined by analyzing a dilution series of XMRV quantitative standard ranging from 8.46 x 109 VP/µl to 1 x 10-1 IU/µl.
• Each dilution has been tested in replicates (n = 4) using Norgen’s XMRV RT-PCR Kit Dx on 1X TAE, 1.7% Agarose gels.
• The linear range of Norgen’s XMRV RT-PCR Kit Dx has been determined to cover concentrations from 9 VP/µl to at least 8 x 106 VP/µl
Storage Conditions and Product Stability
|Title||Detection of Xenotropic Murine Leukemia Virus-Related Virus in Prostate Biopsy Samples.|
|Journal||J Coll Physicians Surg Pak. 2014.|
|Authors||Faraz Ahmed Baig, Talat Mirza, Rafiq Khanani and Saeed Khan.|
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