A ready-to-use system for the isolation and detection of XMRV viral RNA from urine using end-point RT-PCR
|Urine-Based XMRV RT-PCR Detection Kit||34700||24 tests|
Urine-Based XMRV RT-PCR Detection Kit
Xenotropic murine leukemia virus-related virus (XMRV) belongs to the family Retroviridae and the genus gammaretrovirus. The virus was first described in 2006 and has since been isolated from human biological samples. XMRV has a single-stranded RNA genome that replicates through a DNA intermediate. The virus gets its name due to its close relationship with the murine leukemia viruses (MuLVs). The viral genome is approximately 8100 nucleotides in length and is 95% identical with several endogenous retroviruses of mice. While gammaretroviruses have well-characterized oncogenic effects in animals, they have not been shown to cause human cancers. However, XMRV was recently discovered in human prostate cancers and is the first gammaretrovirus known to infect humans. In addition to prostate cancer, a possible association with chronic fatigue syndrome has been reported, however it has yet to be established whether XMRV is a cause of this disease. The causal role of XMRV in cancer has yet to be established and the virus does not appear to be capable of transforming cells directly.
Norgen’s Urine-Based XMRV RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of XMRV from urine. First the kit contains components for the rapid isolation of total RNA, including viral RNA, from the urine samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second the kit contains XMRV RT-PCR Master Mix and controls for PCR Amplification, as well as a Control RT-PCR Master Mix to allow for amplification of both an Isolation Control and a PCR Control. The amplified PCR products are then detected using agarose gel electrohporesis. Alternatively, detection can be performed by real-time PCR using melt curves (Detection). Please see the flowchart to the right.
The XMRV Master Mix contains reagents and enzymes for the specific amplification of a 300 bp region of XMRV. In addition, Norgen’s Urine-Based XMRV RT-PCR Detection Kit contains a second Master Mix, the Control 2x RT-PCrR Master Mix, which can be used to identify possible PCR inhibition and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples. the XMRV RT-PCR Primer Set and Controls are also available separately for end-point RT-PCR detection.
• The linear range of Norgen’s XMRV RT-PCR Kit Dx was determined by analyzing a dilution series of XMRV quantitative standard ranging from 8.46 x 109 VP/µl to 1 x 10-1 IU/µl.
• Each dilution has been tested in replicates (n = 4) using Norgen’s XMRV RT-PCR Kit Dx on 1X TAE, 1.7% Agarose gels.
• The linear range of Norgen’s XMRV RT-PCR Kit Dx has been determined to cover concentrations from 9 VP/µl to at least 8 x 106 VP/µl
Urine-Based XMRV PCR Detection Kit Contents
RNA Lysis Solution
RNA Wash Solution
RNA Elution Solution
Mini Filter Spin Columns
Elution tubes (1.7 mL)
XMRV 2x RT-PCR Master Mix
Control 2x RT-PCR Master Mix
Isolation Control (IsoC)
XMRV Positive Control (PosC)
Norgen’s DNA Marker
XMRV RT-PCR Primer Set and Controls Contents
XMRV Primer set
XMRV Positive Control (PosC)
Storage Conditions and Product Stability
|Title||Detection of Xenotropic Murine Leukemia Virus-Related Virus in Prostate Biopsy Samples.|
|Journal||J Coll Physicians Surg Pak. 2014.|
|Authors||Faraz Ahmed Baig, Talat Mirza, Rafiq Khanani and Saeed Khan.|
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