Fast and reliable purification of genomic and apoptotic DNA from urine

  • Rapid isolation of both small and large species of DNA from urine
  • Convenient spin column format
  • Effective removal of PCR inhibitors
  • Purified DNA is highly suited to sensitive downstream applications
  • Allows for the purification of viral DNA from urine
  • Small urine input ranging from as low as 50 µL to 1.75 mL

ItemsCat. #Size
Urine DNA Isolation Kit 18100 50 preps

Urine DNA Isolation Micro Kit

This kit provides a fast, reliable and simple procedure for isolating DNA from up to 1.75 mL of urine. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. Multiple samples can be processed in 30 minutes. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.

This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.


DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.

Kit Specifications
Volume of Urine Processed
1.75 mL
Average Yield*
Up to 50 ng
Size of DNA Purified
Large (> 1 kb)
and small (150-250 bp)
Time to Complete 10 Purifications
30 minutes hands-on time
(plus a 1 hour incubation)

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Norgen’s Urine DNA Isolation Kit contains ready-to-use Proteinase K and Pronase solutions, which are dissolved in a specially prepared storage buffer. The Proteinase K and the Pronase are stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K and Pronase, storage at 2–8°C is recommended.

Kit Components
Component Cat. 18100 (50 preps)
Binding Solution K 15 mL
Proteinase K in Storage Buffer 2 mL
Pronase K in Storage Buffer 2 mL
Soluton WN 9 mL
Wash Solution D 30 mL
Wash Solution B 15 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 100
Product Insert 1

Title Epigenetic silencing of the MUPCDH gene as a possible prognostic biomarker for cyst growth in ADPKD
Journal Scientific Reports. 2015.
Authors Yu Mi Woo, Yubin Shin, Jung-Ah Hwang, Young-Hwan Hwang, Sunyoung Lee, Eun Young Park, Hyun Kyung Kong, Hayne Cho Park, Yeon-Su Lee & Jong Hoon Park
Title Long-Term Frozen Storage of Urine Samples: A Trouble to Get PCR Results in Schistosoma spp. DNA Detection?
Journal PLoS ONE. 2013.
Authors Pedro Fernández-Soto, Virginia Velasco Tirado, Cristina Carranza Rodríguez, José Luis Pérez-Arellano, Antonio Muro
Title RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids.
Journal The EMBO Journal. 2012.
Authors Abdullah Z, Schlee M, Roth S, Mraheil MA, Barchet W, B?ttcher J, Hain T, Geiger S, Hayakawa Y, Fritz JH, Civril F, Hopfner KP, Kurts C, Ruland J, Hartmann G, Chakraborty T, Knolle PA.
Title Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions.
Journal The Open Biomarkers Journal. 2011.
Authors Beermann A, Ghanjati F, Hermanns T, Poyet C, Pereira J, Fischer J, Wernet P and Santourlidis S.
Title Renal kallikrein excretion and epigenetics in human acute kidney injury: Expression, mechanisms and consequences.
Journal BMC Nephrology.. 2011
Authors Kang SW, Shih PA, Mathew RO, Mahata M, Biswas N, Rao F, Yan L, Bouchard J, Malhotra R, Tolwani A, Khandrika S, Mehta RL, O'Connor DT.