Fast and reliable purification of genomic and apoptotic DNA from urine
|Urine DNA Isolation Kit||18100||50 preps|
Urine DNA Isolation Micro Kit
This kit provides a fast, reliable and simple procedure for isolating DNA from up to 1.75 mL of urine. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. Multiple samples can be processed in 30 minutes. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.
This kit is fully compatble with Norgen's Urine Collection and Preservation Tubes.
DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.
Volume of Urine Processed
Up to 50 ng
Size of DNA Purified
Large (> 1 kb)
and small (150-250 bp)
|Time to Complete 10 Purifications||
30 minutes hands-on time
(plus a 1 hour incubation)
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Norgen’s Urine DNA Isolation Kit contains ready-to-use Proteinase K and Pronase solutions, which are dissolved in a specially prepared storage buffer. The Proteinase K and the Pronase are stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K and Pronase, storage at 2–8°C is recommended.
|Title||Long-Term Frozen Storage of Urine Samples: A Trouble to Get PCR Results in Schistosoma spp. DNA Detection?|
|Journal||PLoS ONE. 2013.|
|Authors||Pedro Fernández-Soto, Virginia Velasco Tirado, Cristina Carranza Rodríguez, José Luis Pérez-Arellano, Antonio Muro|
|Title||RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids.|
|Journal||The EMBO Journal. 2012.|
|Authors||Abdullah Z, Schlee M, Roth S, Mraheil MA, Barchet W, B?ttcher J, Hain T, Geiger S, Hayakawa Y, Fritz JH, Civril F, Hopfner KP, Kurts C, Ruland J, Hartmann G, Chakraborty T, Knolle PA.|
|Title||Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions.|
|Journal||The Open Biomarkers Journal. 2011.|
|Authors||Beermann A, Ghanjati F, Hermanns T, Poyet C, Pereira J, Fischer J, Wernet P and Santourlidis S.|
|Title||Renal kallikrein excretion and epigenetics in human acute kidney injury: Expression, mechanisms and consequences.|
|Journal||BMC Nephrology.. 2011|
|Authors||Kang SW, Shih PA, Mathew RO, Mahata M, Biswas N, Rao F, Yan L, Bouchard J, Malhotra R, Tolwani A, Khandrika S, Mehta RL, O'Connor DT.|
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