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On Ready To Use PCR Master Mix

Optimized for robust PCR or RT-PCR amplification

Checkout Our PCR Master Mixes

  • Ready-to-Use TaqMan Master Mixes with pre-mixed internal PCR control (HEX/VIC)
  • Both Taqman PCR Master Mix and RT-PCR Master Mixes available
  • Use with Norgen's TaqMan Probe/Primer and Control Sets
  • For any PCR application where the input template quality is important to avoid false negatives
  • Convenience and time savings
  • Cost efficient
  • High sensitivity

What's the Deal?

Get 40% off Cat.28007, 28113, 28114, and 28115 kits. Simply enter the code MASTER40 at checkout.*

*North American customers only. For other countries, please contact your local distributor for country-specific promotions. See our full list of distributors here

2X One-Step RT-PCR Master Mix

Product Number Reactions Order
28113 100 reactions
28114 200 reactions
28115 500 reactions

2X PCR Master Mix

Product Number Reactions Order
28007 100 reactions

More Information

2X One-Step RT-PCR Master Mix

Norgen's 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen's 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen's 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.

Norgen's 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:

Cat # 28113 - 100 reactions (sufficient for 100 reactions x 20 μL reaction volume)
Cat # 28114 - 200 reactions (sufficient for 200 reactions x 20 μL reaction volume)
Cat # 28115 - 500 reactions (sufficient for 500 reactions x 20 μL reaction volume)

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PCR, RT-PCR Master Mix on sale 40% off