Pure RNA, Pure Results.Exploring the power of RNA Get Your Free Sample! Request Quote Why Our Powerful and Highly-Sensitive RNA Extraction Technology is Best-in-Class No RNA Carriers Required RNA carriers negatively impact the efficacy of downstream applications like NGS. Our RNA extraction technology is highly sensitive & linear down to a few cells without the need for carrier RNA. No Hazardous Compounds Carryover of hazardous compounds such as Phenol/Chloroform can negatively impact your downstream results. Unlike other common extraction methods, our RNA extraction technology does not require the use of such hazardous compounds. Size (and Content) Matters Silica has shown a bias towards sequences that have a high molecular weight and a high GC content. Our RNA extraction technology shows no bias towards GC content or molecular weight, providing a true representation of RNA content. Trusted by Leading Institutions Worldwide "We have been using Norgen's RNA kits for many years for our colorectal cancer diagnosis projects and their products proved to be very efficient in RNA extraction. Norgen's products worked perfectly and helped us to engage in new molecular approaches." — Italian Institute for Genomic Medicine Figure 1 / 3 Previous High Quality of Isolated RNA and Complete Size Range Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 μL of the 50 μL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation. Superior Diversity of miRNA Detected from Plasma Figure 2. Superior Diversity of miRNA Detected from Plasma. Norgen's Total RNA Purification Kit isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 μL of plasma using Norgen's Total RNA Purification Kit or 625 μL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitors miRNA kit (right). Image courtesy of LC Sciences, Houston. Linear and Sensitive Isolation of Both Large and Small RNA Figure 3. Linear and Sensitive Isolation of Both Large and Small RNA. Norgens Total RNA Purification Kit allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 μL isolated RNA was then subjected to a 20 μL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 μL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen's Total RNA Purification had the best linearity (higher R2) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21). Next Click for expanded view Filter By Please enable JavaScript in order to filter products Format: Spin Column This format utilizes Norgen's proprietary RNA extraction technology for single column-based extractions. High Throughput This format utlizes Norgen's best-in-class RNA purification technology in a 96-well plate for high-throughput extractions. Sample Type or Starting Material: Cells Animal Tissue Blood Bacteria Yeast Fungi Plant Tissue Urine LCM Input Amount: Please select a sample type to see options Cells: Up to 2 x 105 cells 2 x 105 - 5 x 105 cells 5 x 105 - 1 x 106 cells 1 x 106 - 3 x 106 cells 3 x 106 - 5 x 109 cells Animal Tissue: Up to 3 mg 3 - 10 mg 5 - 25 mg 10 - 15 mg 15 - 20 mg 20 - 25 mg 25 - 30 mg 50 - 250 mg Blood: Up to 100 μL 100 μL - 650 μL 10 μL - 2 mL 2 - 3 mL 3 - 10 mL Bacteria: Up to 1 x 109 cells 1 x 109 - 2.5 x 109 cells Yeast: Up to 1 x 108 cells 1 x 108 - 2 x 108 cells Fungi: Up to 40 mg 40 - 50 mg 50 - 1000 mg Plant Tissue: Up to 40 mg 40 - 50 mg 50 - 100 mg 100 - 1000 mg Urine: 0.5 - 1 mL 5 - 10 mL 1 - 50 mL LCM: Up to 2 x 105 cells 2 x 105 - 5 x 105 cells Includes: gDNA removal column Usage: Research Diagnostic Size of Purified RNA: Total RNA (All sizes, including small RNA) microRNA (< 200 nt) Shop Total RNA ExtractionShowing 10 of 10 products Total RNA Purification Kit Dx Cat. Dx17200, Dx24300, Dx24350, Dx24380 Isolation and purification of total RNA from tissue samples, blood, plasma, serum, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use View Product Page Total RNA Purification Kit Cat. 17200, 37500, 17250, 17270, 24300, 24350, 24370, 24380 Rapid purification of total RNA - including microRNA - without the use of phenol View Product Page Total RNA Purification Plus Kit Cat. 48300, 48400 Purification of total RNA - including microRNA - with rapid gDNA removal View Product Page Total RNA Purification Micro Kit Cat. 35300, 35350 Rapid purification of total RNA - including microRNA - from small input amounts View Product Page Total RNA Purification Plus Micro Kit Cat. 48500 Rapid purification of total RNA - including microRNA - from small input amounts View Product Page Total RNA Purification Maxi Kit Cat. 26800 Rapid purification of total RNA - including microRNA - without the use of phenol View Product Page Single Cell RNA Purification Kit Cat. 51800 Rapid purification of total RNA - including microRNA - from small input amounts View Product Page microRNA Purification Kit Cat. 21300 Rapid purification of microRNA without phenol View Product Page Animal Tissue RNA Purification Kit Cat. 25700 Purification of total RNA (including microRNA) from all types of tissues including fiber-rich tissues View Product Page Fatty Tissue RNA Purification Kit Cat. 36200 Purification of total RNA (including microRNA) from animal tissues with high lipid content View Product Page Get My Free Sample See the benefits of Norgen's RNA Extraction Technology in your lab. Get My Sample Select Publications Title Combined MEK and JAK/STAT3 pathway inhibition effectively decreases SHH medulloblastoma tumor progression Journal Communications Biology Authors Zagozewski, J.; Borlase, S.; Guppy, B.J.; et al. Title A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing Journal Molecular Cancer Authors El Fatimy, R.; Zhang, Y.; Deforzh, E.; et al. Title Multiomic analysis of microRNA-mediated regulation reveals a proliferative axis involving miR-10b in fibrolamellar carcinoma Journal JCI Insight Authors Francisco, A.; Kanke, M.; Massa, A.; et al. Title Blood circulating miR-28-5p and let-7d-5p associate with premature ageing in Down syndrome Journal Science Direct Authors Morsiani, C.; Bacalini, M.G.; Collura, S.; et al. Title Contributions of Circulating microRNAs for Early Detection of Lung Cancer Journal Cancers 2022 Authors Vykoukal, J.; Fahrmann, J.F.; Patel, N.; et al. Title Synergistic Analysis of Circulating Tumor Cells Reveals Prognostic Signatures in Pilot Study of Treatment-Naïve Metastatic Pancreatic Cancer Patients Journal Biomedicines 2022 Authors Owen, S.; Prantzalos, E.; Gunchick, V.; et al. Title Validation of Isolation Methodology and Endogenous Control Selection for qRT-PCR Assessment of Microrna Expression in Serum and Urine Exosomes Journal Blood 2014 Authors Crossland, R.E.; Norden, J.; Bibby, L.; et al. Title Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta Journal European Journal of Plant Pathology 2016 Authors Kałużna, M.; Kuras, A.; Mikiciński, A.; Puławska, J. Title Comparison of Whole Blood RNA Preservation Tubes and Novel Generation RNA Extraction Kits for Analysis of mRNA and MiRNA Profiles Journal PLOS ONE 2014 Authors Häntzsch, M.; Tolios, A.; Beutner, F.; Nagel, D.; Thiery, J.; et al. Title Simultaneous extraction of mRNA and microRNA from whole blood stabilized in tempus tubes Journal BMC Res Notes 2019 Authors Richards, J.; Unger, E.R.; Rajeevan, M.S. Title Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal Journal BMC Biotechnology 2018 Authors Brown, R.A.M.; Epis, M.R.; Horsham, J.L.; et al. Talk to the #RNAExperts about Norgen's Best-in-Class RNA Extraction Technology Let's Talk
Figure 1 / 3 Previous High Quality of Isolated RNA and Complete Size Range Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 μL of the 50 μL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation. Superior Diversity of miRNA Detected from Plasma Figure 2. Superior Diversity of miRNA Detected from Plasma. Norgen's Total RNA Purification Kit isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 μL of plasma using Norgen's Total RNA Purification Kit or 625 μL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitors miRNA kit (right). Image courtesy of LC Sciences, Houston. Linear and Sensitive Isolation of Both Large and Small RNA Figure 3. Linear and Sensitive Isolation of Both Large and Small RNA. Norgens Total RNA Purification Kit allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 μL isolated RNA was then subjected to a 20 μL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 μL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen's Total RNA Purification had the best linearity (higher R2) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21). Next Click for expanded view