Saliva RNA Collection & Preservation Device New Price Description Specification Products Norgen's Saliva RNA Collection and Preservation Devices Dx are designed for 1) safe and non-invasive saliva collection and 2) preservation of RNA in saliva samples at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices Dx consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen's Saliva RNA Preservative contained within a sealed squeezable ampoule. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen's Saliva/Swab RNA Purification Kits Dx (Cat# 69100/69300). Each of Norgen's Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for in vitro diagnostic use for medical purposes. Non-invasive collection procedure — just spit, twist, test Viral inactivation for safe transport & handling Tested on SARS-CoV-2 virus Inactivates virus within 5 minutes of mixing CE-IVD marked version available Authorized for sale by Health Canada Suitable for self-collection of saliva samples supervised by a trained individual in the collection procedure by watching the training video and reading over the collection instructions. Saliva RNA Collection and Preservation Devices Dx (53800) Saliva RNA Collection and Preservation Devices (RU53800)
Total Nucleic Acid Preservation Tubes Discount Decription Specification Products Norgen's Total Nucleic Acid Preservation Tubes Dx are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab. The Total Nucleic Acid Preservation Tubes contain Norgen's Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months. This kit is ideal for collecting and preserving nasal and oral swab samples for in vitro diagnostic use for medical purposes. Norgen's Total Nucleic Acid Preservation Tubes Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen's Total Nucleic Acid Preservation Tubes Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate these devices in conjunction with a downstream in vitro diagnostic assay. No need to immediately process samples Total DNA, including viral DNA, preserved at room temperature over 4 months Total RNA, including viral RNA, preserved at room temperature over 2 months Inactivate microorganisms including bacteria, fungi, yeast and viruses Ship swab samples at room temperature safely Compatible with most DNA and Viral RNA isolation methods CE-IVD marked version available Authorized for sale by Health Canada Total Nucleic Acid Preservation Tubes Dx (Dx69200) Total Nucleic Acid Preservation Tubes (69200)
Saliva/Swab RNA Purification Kits Decription Specification Products Norgen's Saliva/Swab RNA Purification Kit provides a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen's Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen's Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen's proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS. Isolate high quality total RNA, including viral RNA, from fresh and preserved saliva and swab samples Fast and easy processing using rapid spin-column format Isolate total RNA, from large rRNA down to microRNA (miRNA) No phenol or chloroform extractions Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA Buffer chemistry inactivates viruses including SARS-CoV-2 High throughput 96-well plate format for rapid purifications is also available Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Authorized for sale by Health Canada CE-IVD marked version available Saliva / Swab RNA Purification Kit Dx (Dx69100) Saliva / Swab RNA Purification Kit (69100) Saliva/Swab RNA Purification 96-Well Kit Dx (Dx69300) Saliva/Swab RNA Purification 96-Well Kit (69300)
Total RNA Purification Kits Description Specification Products Norgen's Total RNA Purification Kit Dx provides a rapid method for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. Any diagnostic results generated using the RNA isolated with Norgen's Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. Extract high quality & purity total RNA including miRNA No phenol step required Bind & elute all RNA irrespective of size or GC content, without bias Very sensitive & linear down to a few cells without the need for carrier RNA Convenient & fast spin column format Isolate from a wide variety of specimens Purified RNA is suitable for a variety of downstream applications Buffer chemistry inactivates viruses including SARS-CoV-2 Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Authorized for sale by Health Canada CE-IVD marked version available Total RNA Purification Kit Dx (Dx17200) Total RNA Purification Kit (17200, 37500, 17250, 17270) Total RNA Purification 96-Well Kit Dx (Dx24300, Dx24350, Dx24380) Total RNA Purification 96-Well Kit (24300, 24350, 24370, 24380)
COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Description Specification Products Norgen's COVID-19/Influenza (A & B) TaqMan RT-PCR Kit includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains SARS-CoV-2 detection target; N (Nucleoprotein), Influenza A detection target; M gene and Influenza B detection target; HA (Hemaglutinin) and the human RNase P transcript as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/Inf A & B/RP Positive Control contains an in vitro RNA transcript for the SARS-related target gene (N Gene), and genes associated with Influenza A (M gene) and Influenza B (HA Gene), as well as the human RP gene (internal control). Multiplex RT-PCR system detects two SARS-CoV-2 specific genes (N gene and ORF1ab) in a single one step RT-PCR reaction Primer & Probe Mixes contain all N gene, ORF1ab and human RP assays Positive Control contains the three SARS-related target genes: N gene, ORF1ab gene as well as the human RP (internal control). Convenient ready-to-use 2X One-Step RT-PCR Master Mix CE-IVD marked version availble COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx (DxTM67300) COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) (TM67300)
COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) Decription Specification Products Norgen's COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) Dx is an in vitro diagnostic test for the qualitative detection of SARS-CoV-2 specific RNA using a multiplexed TaqMan® fluorescence detection assay (FAM and HEX/VIC) based on the Charité/Berlin protocol. The assay is designed for use with RNA isolated from nasopharyngeal swabs, oropharyngeal swabs and saliva samples collected from individuals with clinical signs/symptoms related to SARS-CoV-2 infection for in vitro diagnostic use. SARS-CoV-2 detection based on the Charité/Berlin protocol (E/RdRP genes) recommended by WHO Primer & Probe Mixes contain all E gene/RP and RdRP gene assays in individual tubes All assays are pre-mixed to the working concentrations recommended by the Charité/Berlin protocol Positive Control contains the three SARS-related target genes: E gene, RdRP gene as well as the human RP (internal control) Convenient ready-to-use 2X One-Step RT-PCR Master Mix Authorized for sale by Health Canada CE-IVD marked version available COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) Dx (DxTM67200) COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) (TM67200, TM67240)
2019-nCoV TaqMan RT-PCR Kit Decription Specification Products Norgen's 2019-nCoV TaqMan RT-PCR Kit is designed for the detection of SARS-CoV-2 specific RNA in a real-time RT-PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of SARS-CoV-2 specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of SARS-CoV-2 infection. Norgen's 2019-nCoV TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The 2019-nCoV TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, 3 target Primer & Probe Mixes, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents. CE-IVD marked in accordance with EU Directive 98/79/EC Authorized for sale by Health Canada Fits into in vitro diagnostic workflows COVID-19 detection based on the assays and protocols developed by the CDC Primer & Probe Mixes contain all 3 CDC developed assays in individual tubes All assays are premixed to the working concentration recommended by the CDC Positive Control contains 2 nCoV nucleocapsid target gene RNA (N1,N2) and RNase P (internal control) which are compatible with the CDC primer/probe set Convenient ready-to-use 2X One-Step RT-PCR Master Mix 2019-nCoV TaqMan RT-PCR Kit Dx (DxTM67100. DxTM67120) 2019-nCoV TaqMan RT-PCR Kit (TM67100, TM67120)
COVID-19/Influenza (A & B) TaqMan RT-PCR Kit Decription Specification Products Norgen's COVID-19/Influenza (A & B) TaqMan RT-PCR Kit includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains SARS-CoV-2 detection target; N (Nucleoprotein), Influenza A detection target; M gene and Influenza B detection target; HA (Hemaglutinin) and the human RNase P transcript as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/Inf A & B/RP Positive Control contains an in vitro RNA transcript for the two SARS-related target genes: N gene, ORF1ab gene as well as the human RP gene (internal control). Multiplex RT-PCR system detects SARS-CoV-2 specific gene (N gene), Influenza A (M gene) and Influenza B (HA gene) in a single one step RT-PCR reaction Primer & Probe Mixes contain all N gene, M gene, HA gene and human RP assays Positive Control contains the SARS-related target genes: N gene, Influenza A target gene (M gene), Influenza B target gene (HA gene) as well as the human RP (internal control) Convenient ready-to-use 2X One-Step RT-PCR Master Mix included in kit Manufactured under ISO 13485:2016 for research use only CE-IVD marked version pending COVID-19/Influenza (A & B) TaqMan RT-PCR Kit (TM67400)
Resources Related Videos Top 5 Reasons to Choose Silicon Carbide RNA Extraction Technology Tutorial: Saliva RNA Extraction with Silicon Carbide Improve your Total RNA Extraction Results with Norgen Biotek's Patented Technology How to Reduce False Negative COVID-19 Test Results in Your Lab Related Videos Saliva Samples: A Viable Alternative to Nasopharyngeal Swabs Top 5 Reasons to Choose Silicon Carbide RNA Extraction Technology Tutorial: Saliva RNA Extraction with Silicon Carbide Improve your Total RNA Extraction Results with Norgen Biotek's Patented Technology Tutorial: Detection of COVID-19 with TaqMan RT-PCR Kit (E/RdRP genes) How to Reduce False Negative COVID-19 Test Results in Your Lab Related Videos Saliva Samples: A Viable Alternative to Nasopharyngeal Swabs Top 5 Reasons to Choose Silicon Carbide RNA Extraction Technology Tutorial: Saliva RNA Extraction with Silicon Carbide Improve your Total RNA Extraction Results with Norgen Biotek's Patented Technology Tutorial: Detection of COVID-19 with TaqMan RT-PCR Kit (E/RdRP genes) How to Reduce False Negative COVID-19 Test Results in Your Lab Recent Blogs The Triple Threat of COVID, Influenza and RSV By Alison Waller, PhD on Tue, 01/16/2024 - 12:00 14 min read COVID-19 In the Northern Hemisphere, winter ushers in the cold and flu season. People with runny noses and sore throats are often debating if they have the flu, "just a cold", or now COVID-19… Continue Reading COVID, The Flu, and You By Zak Mason on Fri, 11/04/2022 - 12:00 5 min read COVID-19InfluenzaPCR By now, everyone has heard of the virus that has changed the world since late 2019. First reported in Wuhan in December of 2019, SARS-CoV-2 spread across the face of the earth, leaving chaos in its… Continue Reading What is PCR Testing? By Zak Mason on Thu, 06/17/2021 - 12:00 6 min read COVID-19PCRRNA In the past, diagnoses were limited to what could be observed by an attending physician with their own senses. As one might expect, this resulted in wildly varying levels of patient care and… Continue Reading How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide By The Norgen Team on Thu, 03/04/2021 - 12:00 4 min read COVID-19RNASilicon Carbide Is it possible that your COVID-19 workflow is not as accurate as you may think? At the start of the COVID-19 pandemic, researchers and laboratories scrambled to develop SARS-CoV-2 test kits in mass… Continue Reading COVID-19 Mass Screening: Tracking Data from Wastewater By Jason Perez on Mon, 02/08/2021 - 12:00 4 min read COVID-19PCRRNA The world as we all know it changed dramatically in the early months of 2020 with the onset of COVID-19. With business closures and hospital overloads occurring around the globe, researchers… Continue Reading Saliva: A Viable Alternative to Nasal and Oral Swabs for the Detection of SARS-CoV-2 By Ben Abraham on Tue, 09/29/2020 - 12:00 6 min read COVID-19SalivaSwab In this blog, you’re going to learn what makes saliva a viable alternative to nasal and oral swabs for the detection of SARS-CoV-2. This post includes statistics on real-life examples of SARS-CoV-2… Continue Reading Coronavirus: To panic or not to panic? By Ben Abraham on Thu, 02/06/2020 - 12:00 2 min read COVID-19PCRRNA Editor's Update (June 20, 2020): The information represented in this article is a representation of when the original article was written on February 26th, 2020 Chances are, you’ve been keeping… Continue Reading