Plant Viroid and Virus Detection Services

Plant Viroid and Virus Detection Services

Norgen Biotek is a leading expert in the molecular diagnostics sector. Norgen has developed over 340 high quality PCR-based detection products, which are sold globally for all types of environmental pathogens. With extensive expertise in sample preparation, sequencing and analysis of every sample type, our plant viroid and virus detection services are seamlessly streamlined for efficient, robust, and high throughput sample processing. As a result, Norgen offers fast turn-around services completed with a comprehensive final report. Norgen’s truly innovative real-time PCR detection system provides detailed quantitative detection results (Severe, High, Moderate, and Low) rather than a simple indication of whether or not a pathogen is present, allowing for more useful data in disease management.

 

Advantages of Norgen's Grapevine Detection Services

  1. Proven GRBV detection system validated by international GRBV experts
  2. Sensitive TaqMan real-time PCR system provides quantification data related to virus infection levels
  3. Mandatory internal PCR controls to QC for all PCR samples and prevent false negative results
  4. High quality virus DNA/RNA purification kits are used to isolate nucleic acids of excellent quality and integrity for improved virus detection sensitivity
 

Current Plant Viroid Detection Services

  • Avocado Sunblotch Viroid (ASBVd)
  • Chrysanthemum Chlorotic Mottle Viroid (CChMVd)
  • Chrysanthemum Stunt Viroid (CSVd)
  • Citrus Exocortis Viroid (CEVd)
  • Coconut Cadang-Cadang Viroid (CCCVd)
  • Coconut Tinangaja Viroid (CTiVd)
  • Hop Stunt Viroid (HSVd)
  • Hop Latent Viroid (HLVd))
  • Pospiviroid
  • Potato Spindle Tuber Viroid (PSTVd)
  • Tomato Chlorotic Dwarf Viroid (TCDVd)
  • Tomato Planta Macho Viroid (TPMVd)

Current Plant Virus Detection Services

  • Grapevine leafroll-associated virus-3 (GLRaV-3)
  • Grapevine red blotch virus (GRBV)
  • Plum Pox Virus (PPV)
  • Grapevine leafroll-associated virus-1 (GLRaV-1)
  • Grapevine Pinot Gris Virus (GPGV))
  • Grapevine fanleaf virus (GFLV)

If the specific pathogen service you require is not listed, please contact us for more information.

info@norgenbiotek.com
905 227 8848

Plant Pathologist
Dr. Won-Sik (Bernard) Kim

 

Devastating Grape Viruses Affecting Economical Impact

Grapevine leafroll-associated virus-3 (GLRaV-3)

  • Currently ranks as one of the most harmful diseases affecting wine grape cultivars
  • Reduced yield, lower fruit sugar levels, higher titratable acidity and lower berry anthocyanin levels (result in lower quality wine)
  • Malybugs are now acknowledged as the dominant vectors of GLRaV-3

Grape red blotch virus
(GRBV)

  • Significant reduction in sugar accumulation
    – up to 5 °Brix
  • Uneven ripening, or failure to ripen, results in poor flavour and/or unripe fruit.
  • Colour development
  • Vector and transmission mechanisms are not clearly known

Most viruses that infect grapevines are graft-transmissible diseases.

 

Challenges in Field Diagnosis

  • Symptoms displayed in the field are rarely unique to a particular disease
  • Some infected vines can be delayed and may not show any symptoms indicative of their disease status
  • Red leaves could be an indication of a virus disease, a fungal root disease, a nutritional deficiency, abiotic stress such as magnesium deficiency, or sulfur burn, or spray damage, or feeding by mites, insects, or rodents
  • In white-fruited varieties symptoms are very difficult to discern

 

Sensitive and Quantitative Grape Virus Detection

 

Year Round Virus Detection Using Various Types of Tissue Samples

 

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Norgen Biotek's Robust and Efficient Sample Preparation Workflow:

Subsampling

Homogenization

DNA/RNA Purification

(DNA and RNA Virus)

Detection

 

Next Generation Sequencing Services

are also available