-500
500
1000
1,500
2,000
2,500
3,000
3,500
4,000
0
-500
500
1,000
1,500
2,000
2,500
3,000
3,500
4,000
0
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
Cycle
PCR Base Line Subtracted RFU
10
4
Cells
10 Cells
10
3
Cells
1 Cell
10
2
Cells
No Template