0.1 mL
0.5 mL
1 mL
15
17
19
21
23
25
27
29
31
33
0
20
40
60
80
100
120
1 mL
0.5 mL
0.1 mL
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
30.27
28.09
27.22
0.67
0.35
0.08
100
91
96
B
A
Different Plasma Volumes
Different Plasma Volumes
Relative Amount of Plasma Free- Circulating miR-21
Average Ct. Value of Plasma Free- Circulating miR-21
% of Linearity