4 mL
5 mL
10 mL
0
20
40
60
80
100
120
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
2.29
2.56
4.66
91
90
100
4 mL
5 mL
10 mL
15
17
19
21
23
25
27
25.38
25.22
24.36
% of Linearity
Different Plasma Volumes
Different Plasma Volumes
Relative Amount of Plasma Free- Circulating miR-21
Average Ct. Value of Plasma Free-Circulating miR-21
A
B