*On purchases of Stool Collection & Preservation Devices and Stool Nucleic Acid isolation kits.
A rapid and simple method for the collection and preservation of stool, crucial for analyses like next-generation sequencing (NGS) and microbiome profiling.
A convenient and rapid method to purify RNA and DNA from both the host cells and the microbes
*Minimum purchase value for each discount tier must be reached through the 8 products listed below, it does NOT refer to total order value. A complete list of "SMART Save" products are listed on this page, under "SMART Save Products". These are the only products that qualify to receive the 40% discount for the third, $2000, discount tier. Promotion available only to customers in Canada or the U.S. Discount not available to Norgen Biotek distributors. Offer cannot be combined with other existing promotions. Offer valid until May 14, 2024.
SKU 27650
SKU 45660
SKU 63700
SKU 45670-B
SKU 27600
SKU 55700
SKU 49500
SKU 45600
Preview Text: Stool, or feces, is a complex substance excreted from the gastrointestinal tract that is composed of digested food, bacteria, fungi, viruses, protozoa, and host cells. The biological, chemical and physical properties of each stool sample vary based on diet, environment, and the disease/health status of the host.
Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods.
Figure 2. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions. The microbial composition from the stool samples preserved in Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period while non-preserved stool samples showed a distance in the dendrogram from the original stool profile.
Figure 1. Higher Yields of DNA than Competitor Q. Stool DNA was isolated from 200 mg of fresh or preserved stool samples using Norgen’s Stool DNA Isolation Kit and Competitor Q's Kit. For evaluation, 10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel. Norgen's kit isolated much higher yields of DNA. *Stool was collected using Norgen’s Stool Nucleic Acid Collection and Preservation tubes (Cat. 45660). Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900).
Figure 2. Yield and Quality of Purified Stool RNA. Total RNA was isolated from 200 mg of human stool using Norgen's Stool RNA Purification Kit and a leading competitor kit. Comparisons were then made based on yield, and A260:A280/A260:A230 ratios measured using the NanoVue Plus™. In Panel A it can be seen that both kits isolated RNA with high A260:A280 ratios (all samples were found to be above 1.8 and below 2.2). In Panel B, Norgen's kit was found to isolate RNA with a high A260:A230 (with all samples once again falling in the 1.8-2.2 range). The competitor kit, however, was found to isolate RNA with extremely low A260:A230 ratios, with none of the samples displaying a A260:A230 ratio higher than 0.20. The results in Panel C are in agreement with the gel photo from Figure 1, and Norgen's kit was found to isolate higher amounts of RNA, with an average yield of 14.58 µg, compared to the competitor’s average of 12.26 µg.
LIMITED TIME OFFER ON STOOL PRODUCTS!
on purchases of Stool Collection & Preservation Devices and Stool Nucleic Acid*
Our team of dedicated scientists will be happy to learn more about your specific project needs and provide you with the best tools for stool sample processing.