Stool Nucleic Acid Collection and Preservation Tubes
A rapid and simple method for the collection, preservation and room temperature shipping of stool samples.
Stool Nucleic Acid Collection and Preservation Tubes
A rapid and simple method for the collection, preservation and room temperature shipping of stool samples.
Register today to receive an exclusive 15% off* on your first order.
Overview
- The preservative provides sample homogeneity eliminating sample variability
- Preserve and transport DNA & RNA safely at ambient temperature
- Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
- No cold shipping/storage needed - hassle-free and cost effective
- Isolate nucleic acids for any application including 16s NGS
- Robust preservation over a range of temperatures
- Customizable with various accessories for easy and safe collection
- Eliminates odor and renders samples safe and non-infectious
Norgen’s Stool Nucleic Acid Collection and Preservation Tubes are designed for the rapid preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended at the arrival to testing facilities until the RNA purification.
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Supporting Data
Kit Specifications
|
|
Stool Input
|
2 g
|
Stability of Stool Nucleic Acids at Room Temperature |
2 years for DNA
7 days for RNA* |
* The RNA stability will vary depending on the samples
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for 2 years after the date of shipment without any reduction in performance.
Kit Components |
Cat. 45660 |
---|---|
Stool Nucleic Acid Collection and Preservation Tubes |
50 |
Product Insert |
1 |
Documentation
Purification and Detection of Bacterial RNA From Preserved Stool Samples - Application Note
Comparative Microbiome Profiles from Fecal Preservation Methods
Gut Microbiome Diversity: Comparison of Stool DNA Preservation Methods
Immediate Viral Inactivation Using Norgen’s Stool Nucleic Acid Preservative
Automation of Stool DNA Isolation on the Hamilton Vantage Using Norgen’s Magnetic Bead Stool Isolation Kit
FAQs
Each tube contains 4 mL (~4.56 grams) of preservative. The scoop is attached to the tube cap for easily collecting and depositing the sample.
The dimensions of each stool collection tube are 76mm (height) x 20mm (diameter).
Tube material is as follows:
- Cap: High Density Polyethylene (HD-PE)
- Stem: Polystyrene (PS)
- Tube: Polypropylene (PP)
Norgen's Stool DNA Isolation Kit, Stool Total RNA Purification Kit, and Stool Nucleic Acid Isolation Kit can be used with fresh stool samples or samples preserved with Stool Collection and Preservation Tubes.
Yes, the preserved stool is compatible for analyses of all these metabolites. This answer is based on several independent academic research articles that used our preservation system and then analyzed the samples for various metabolites. Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.
Yes, Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.
Yes, microRNA is also preserved after stool is properly homogenized with the preservative. Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.
According to protocol, the tube can be store between -20°C to -70°C in order to stabilize the RNA for longer period of time. Alternatively, the end-user can aliquot the small volume of homogeneous mixture (after sample collection and mixing) from the stool tube to microcentrifuge tube and then store at -80°C. This aliquoting method also prevents the multiple freeze and thaw cycles that may impact on RNA quality.
Citations
Title | Dietary autolysed yeast modulates blood profiles, small intestinal morphology and caecal microbiota of weaning pigs |
Journal | animal. 2022. |
Authors | S. Namted, K.Poungpong, W. Loongyai, C. Rakangthong, C. Bunchasak |
Title | Dynamics of gut microbiota and short-chain fatty acids during a cycling Grand Tour are related to exercise performance and modulated by dietary intake |
Journal | medRxiv. 2022. |
Authors | Manuel Fernández-Sanjurjo, Javier Fernández, Pablo Martínez-Camblor, et al. |
Title | Impact of probiotic supplementation on exercise endurance among non-elite athletes: study protocol for a randomized, placebo-controlled, double-blind, clinical trial |
Journal | Trials. 2022. |
Authors | Caitlin E. McDermott, Heather K. Vincent, Anne E. Mathews, Brunella Gonzalez Cautela, Mariana Sandoval, Annie Tremblay & Bobbi Langkamp-Henken |
Title | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
Journal | Microorganisms. 2022. |
Authors | Alessandra De Giani, Anna Sandionigi, Jessica Zampolli, Angela Michelotti, Francesco Tursi, Massimo Labra and Patrizia Di Gennaro |
Title | Effectiveness of Multistrain Probiotic Formulation on Common Infectious Disease Symptoms and Gut Microbiota Modulation in Flu-Vaccinated Healthy Elderly Subjects |
Journal | BioMed Research International. 2022. |
Authors | Anna Sandionigi, Alessandra De Giani, Francesco Tursi, Angela Michelotti, Enza Cestone, Silvana Giardina, Jessica Zampolli, and Patrizia Di Gennaro |
Title | Development of a rational framework for the therapeutic efficacy of fecal microbiota transplantation for calf diarrhea treatment |
Journal | Microbiome. 2022. |
Authors | Islam J, Tanimizu M, Shimizu Y, Goto Y, Ohtani N, Sugiyama K, Tatezaki E, Sato M, Makino E, Shimada T, Ueda C, Matsuo A, Suyama Y, Sakai Y, Furukawa M, Usami K, Yoneyama H, Aso H, Tanaka H, Nochi T |
Title | Human Stool Preservation Impacts Taxonomic Profiles in 16S Metagenomics Studies |
Journal | Frontiers in Cellular and Infection Microbiology. 2022. |
Authors | A. Plauzolles, E. Toumi, M. Bonnet, G. Pénaranda, G. Bidaut, L. Chiche, J. Allardet-Servent, F. Retornaz, B. Goutorbe, & P. Halfon |
Title | 2’-Fucosyllactose Ameliorates Chemotherapy-Induced Intestinal Mucositis by Protecting Intestinal Epithelial Cells Against Apoptosis |
Journal | Cellular and Molecular Gastroenterology and Hepatology. 2021. |
Authors | Gang Zhao, Jessica Williams, M. Kay Washington, Yaohua Yang, Jirong Long, Steven D. Townsend, Fang Yan |
Title | Air pollution exposure is associated with the gut microbiome as revealed by shotgun metagenomic sequencing |
Journal | Journal of Environmental Management. 2020. |
Authors | Farnaz Fouladi, Maximilian J. Bailey, William B. Patterson, Michael Sioda, Ivory C. Blakley, Anthony A. Fodor, Roshonda B. Jones, Zhanghua Chen, Jeniffer S. Kim, Frederick Lurmann, Cameron Martino, Rob Knight, Frank D. Gilliland, Tanya L. Alderete |
Title | Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples |
Journal | ASM Journals. 2019. |
Authors | Sonia Tarallo, Giulio Ferrero, Gaetano Gallo, Antonio Francavilla, Giuseppe Clerico, Alberto Realis Luc, Paolo Manghi, Francesca Cordero |
Title | Bacteroidales markers for microbial source tracking in Southeast Asia |
Journal | Water Research. 2017. |
Authors | Jean Pierre Nshimyimana, Mercedes C. Cruz, R. Janelle Thompson, Stefan Wuertz |