Name: Adenovirus Purification Kit
Brand: Norgen Biotek Corp.
SKU: 67600
Price: 839.000000 USD
Availability: InStock

Supporting Data

Figure 1. HeLa cells were infected with the eluted adenovirus vector expressing the beta galactosidase reporter gene. The cells were fixed and stained 48 hours post infection. The dark staining indicates cellular transduction by the adenoviral vector and expression of the beta galactosidase reporter gene.

Figure 2. Total adenoviral vector eluted for 1, 3, or 15 mL of supernatant containing adenovirus. An increase in titer is observed as the volume of adenovirus containing supernatant increases, demonstrating effective purification at different volumes.

Figure 3. Adenovirus vector was eluted from the kit from various volumes of supernatant: 1 mL, 3 mL, and 15 mL. Three separate elutions (500 µL each) were performed with elution buffer P to elute the adenoviral vector.

Kit Specifications
Resin Binding Capacity (total per kit)	At least 3x10¹¹ adenovirus particles as determined by qPCR At least 3x10⁸ transducing units as determined by transduction assay
Input type	Cells, media
Input volume (Supernatant)	1 - 33.5 mL SN per prep (500 mL SN in total)
Input Volume (Cell pellet)	1 mL cell pellet per prep (15 mL in total)
Minimum elution volume	1.3 mL per prep
Time to complete purification	2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction	Yes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 67600 (15 preps)
Lysis Buffer S	5.5 mL
HL-SAN Nuclease	102 µL
Binding Buffer A	20 mL
Purification Solution C	60 mL
Purification Solution D	130 mL
Wash Solution C	2 x 130 mL
Slurry E	12.5 mL
Elution Buffer P (store at 4°C)	66 mL
Protein Neutralizer	4 mL
Elution tubes (1.7 mL)	50
Product Insert	1

Adenovirus Purification Kit