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Applications Notes

The following Application Notes have been developed to offer additional resources to the researchers and scientists who use our kits. Norgen is pleased to share this knowledge with our fellow researchers, scientists, and collaborators.

Spanning a variety of topics and categories, these Notes were brought about by collaborations, customer inquiry, conference posters, as well as to make revisions to industry guidelines (ex. App Note 47 - Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input).

Our Notes share advances in technology, product observations and conclusions, proven validity and outcomes, and comparative education.

If you have questions about a product or service mentioned within an Application Note, contact us. We’re here to assist.


App Note Search



No. Category Application Note Related Kits
001 Protein Rapid Concentration of Dilute Protein Solutions Using the ProteoSpin™ CBED Kit 22800
002 Protein Rapid Desalting of Protein Solutions Using the ProteoSpin™ CBED Kit 22800
003 Protein Efficient Removal of Both Free and Tightly-Bound SDS from Protein Solutions using the ProteoSpin Detergent Clean-up Kit 22800
004 Protein Efficient Removal of Triton X-100 Prior to MALDI-TOF and Digestion Using the ProteoSpin Detergent Clean-up Kit 22800
005 Protein Screening for Bacterial Clones Expressing Inclusion Body Proteins 10300
006 Protein A Time Course Induction Study of Recombinant Protein Expression in E.coli 10300
008 DNA
Ladders		 DNA Quantification by Gel Densitometry with Norgen DNA Ladders 12800
009 RNA Isolation of Fractionated RNA from HeLa Cells Using Norgen's Cytoplasmic & Nuclear RNA Purification Kit 21000
010 DNA Isolation of DNA from as Little as 25 uL of Urine Using Norgen's Urine DNA Isolation kit 18100
011 RNA Isolation and Detection of RNA from as Little as a Single Animal Cell Using Norgen's Total RNA Purification Kit 17200
012 Multiple Analyte Sequential Isolation and Purification of RNA, DNA, and Protein from a Single Sample of Animal Cells using Norgen's RNA/DNA/Protein Purification Kit 47700
013 RNA Isolation and Detection of RNA from as Little as a Single Animal Cell Using Norgen's Total RNA Purification Kit 21300
014 Plant RNA Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit 25800
015 Urine DNA Isolation of High Quality Urinary DNA using Norgen's Urine DNA Isolation Kit 18100
016 Plant microRNA Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit 25800
017 FFPE RNA Rapid Method for the Purification of Total RNA from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Samples 25300
018 RNA, DNA, Protein Sequential Purification of RNA, DNA, and Protein from a Single Sample using Norgen's RNA/DNA/Protein Purification Kit and Comparison to a Market Competitor 47700
019 Leukocyte RNA Rapid Isolation and Purification of Total Leukocyte (White Blood Cell) RNA from Mammalian Blood Samples 21200
020 RNA High-Throughput Purification of Total RNA Including miRNA without the use of Phenol 24300
021 Urine DNA Isolation of High Quality Urinary DNA Using Norgen's Urine DNA Mini Slurry Kit 48800
022 Total RNA Isolation of True Total RNA using the Tempus Blood RNA Tube and Norgen's Total RNA Purification Kit 17200
023 Saliva DNA Efficient Restriction Enzyme Digestion of Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, Isolation Kit 35700
024 Saliva DNA Long Term Stability of DNA Stored in Norgen's Saliva DNA Preservative 12100, 35700
025 Saliva DNA The Range of DNA Yield with Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 35700
026 Saliva DNA High Efficiency Real-Time PCR from Saliva DNA 35700
027 Saliva DNA Sex Determination from Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 35700
028 Saliva DNA Comparison of Norgen's Saliva DNA Collection, Preservation and Isolation Kit to a Leading Competitor's Kit 35700
029 Saliva DNA Sequencing Analysis of Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 3570014400
030 Saliva DNA Isolation of High Quality Saliva DNA from Variable Donor Saliva Samples 35700, 12100
031 Saliva DNA The Range of DNA Yield and Purity with Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 35700
032 Saliva DNA Inhibitory Effect of Norgen's Saliva DNA Preservation on the Growth of Bacteria and Yeast 35700
033 Urine DNA The Range of DNA Yield and Purity with Norgen's Urine DNA Concentration, Preservation, and Isolation Kit see Urine Products*
034 Urine DNA High Efficiency Real-Time PCR from Urine DNA see Urine Products*
035 Urine DNA Efficient Restriction Enzyme Digestion of Urine DNA Isolated using Norgen's Urine DNA Concentration, Preservation, and Isolation Kit see Urine Products*, 12800
036 Urine RNA The Range of RNA Yield and Purity with Norgen's Urine RNA Concentration, Preservation, and Isolation Kit see Urine Products*
037 Urine RNA The Range of RNA Yield and Purity with Norgen's Urine RNA Concentration, Preservation, and Isolation Kit see Urine Products*
038 Urine RNA Long Term Stability of RNA Stored in Norgen's Urine Preservation Buffer 18116
039 Urine Protein The Range of Protein Yield with Norgen's Urine Protein Concentration, Preservation, and Isolation Kit see Urine Products
041 Urine DNA The Range of DNA Yield and Purity with Norgen's Urine DNA Concentration, Preservation, and Isolation Kit (For Resource Limited Settings) see Urine Products
042 Urine RNA The Range of RNA Yield and Purity with Norgen's Urine DNA Concentration, Preservation, and Isolation Kit (For Resource Limited Settings) see Urine Products
043 Saliva DNA Column-Based Isolation of DNA from Preserved Saliva Samples Offers High Quality, Consistent, and Rapid DNA Isolation 35700, RU45400
044 Saliva DNA DNA Isolation from Saliva Preserved using Norgen's Saliva DNA Collection and Preservation Device using Beckman Coulter Agencourt DNAdvance Kit RU45400, RU35700, RU49000
045 RNA Supplementary Protocol for Total RNA Isolation from Staphylococcus aureus-Related Bacteria and Hard-to-Lyse Bacteria Species 17200, 26533, 47700
046 DNA The Effect of Sample Preparation on Humic Acid Removal from Peat Samples: A Comparative Study 64000
047 RNA Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input 17200, 36200, 25300
048 Saliva DNA DNA Isolation from Saliva Preserved with Norgen's Saliva DNA Collection and Preservation Device using Qiagen's QIAmp DNA Blood Mini Kit RU45400, RU35700, RU49000
049 RNA Effect of RNA Isolation Method on microRNA Quantity and Quality in Plasma: A Comparative Study 17200
050 RNA A Comparative Study Between Two Saliva DNA Preservation Systems, and Two Column-Based Saliva DNA Purification Methodsy RU45400
051 SalivaDNA The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit 35700
052 AAV DNA The Isolation of High Quality Stool DNA and its Application to Quantitative Adenovirus Detection using TaqMan® Real-Time PCR 27600, 45600, EP42600
053 DNA Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits 50600, 12100, 12800,
054 Saliva DNA Linearity of DNA Isolated from Increasing Volumes of Preserved Saliva Using Norgen’s Saliva DNA Isolation Kit RU45400, RU35700, RU49000
055 Saliva DNA The Effect of Elution Volume on DNA Quantity and Quality Using Norgen’s Saliva DNA Isolation Kit RU45400, RU35700, RU49000
056 Saliva DNA Comparative Study of DNA Isolated from Saliva Preserved in Norgen’s Preservative Using Norgen’s Saliva DNA Isolation Kit Versus Competitor’s DNA Blood Mini Kit RU45400, RU35700, RU49000
057 StoolDNA Comparative Study of DNA Isolated from Stool Using Norgen’s Stool Nucleic Acid Isolation Kit and Norgen’s Stool DNA Isolation Midi Kit (Slurry Format) Versus Competitor’s DNA Stool Mini Kit 27600, 45600
058 StoolDNA Comparison of Stool DNA Isolation Methods for Bacterial and Mammalian DNA Detection 45600
059 BloodDNA Comparative Study of DNA Isolated from Increasing Volumes of Blood Using Norgen’s Blood Genomic DNA Isolation Kit Versus Competitor’s DNA Blood Mini Kit 46300
060 StoolDNA DNA Isolation from 1 Gram of Stool Using a Silicon Carbide Slurry Method: Comparison to 250mg of Stool and Identification of RNA Presence in Low Molecular Weight DNA Fraction 45600
061 Plasma/Serum DNA Comparative Study of Plasma DNA Isolated Using Norgen’s Plasma/Serum Circulating DNA Purification Kits Versus Competitor’s DNA Blood Kits see Plasma/Serum Kits, 45600
062 Saliva DNA Stability of DNA Stored in Norgen’s Saliva DNA Preservative for Over 30 Months RU45400, 12100
063 Saliva DNA Stability of DNA Stored in Norgen’s Saliva DNAPreservative at 55°C RU45400, 12100
064 Single Cell RNA Isolation of High Quality RNA from Single Mammalian Cell 51800
065 Blood DNA Genomic DNA Isolation from blood collected on different anticoagulants: Citrate, EDTA & Heparin 46300
066 StoolDNA Detection of Viral, Bacterial and Human Genomic DNA from Preserved Stool Samples 27600
067 StoolDNA Purification and Detection of Bacterial RNA from Preserved Stool Samples 27600
068 Plant DNA Detection of Genomic DNA from Processed Plant Oils and Wheat Flour 26200
069 Blood DNA Comparative Study of DNA Isolated from Whole Blood Using Norgen’s Blood Genomic DNA Isolation Mini Kit and Competitors’ Kits 46300
070 Blood DNA Genomic DNA Isolation from Buffy Coat using Norgen’s Blood Genomic DNA Isolation Mini Kit 46300
071 Blood DNA Genomic DNA Isolation from 1 µL – 100 µL of Whole Blood using Norgen’s Blood Genomic DNA Isolation Micro Kit 31200*
072 Blood DNA Effect of Elution Volume on DNA Recovery and Quality using Norgen’s Blood Genomic DNA Isolation Micro Kit 31200*
073 Blood DNA Single Elution vs. Re-Elution: Implications on Concentration and Purity using the Blood Genomic DNA Isolation Micro Kit 31200*
074 Blood DNA Isolation of Genomic DNA from Dried Blood Collected on Different Materials: Blood Cards, Cloth and Solid Surface using Norgen’s Dried Blood Spot Genomic DNA Isolation Kit 36000*
075 Blood DNA Isolation of DNA from Hair Follicles and Hair Shafts using Norgen’s Dried Blood Spot Genomic DNA Isolation Kit 36000*
076 Cells and Tissue DNA Isolation of DNA from 0.1 – 3 million HeLa Cells using Norgen’s Cell and Tissue Genomic DNA Isolation Kit 53100
077 Soil DNA Norgen’s Soil DNA Isolation Kit Outperforms a Competitor with Effective Humic Acid Removal for Metagenomic Studies 64000
078 Saliva DNA Stability of DNA Stored in Norgen’s Saliva DNA Preservative for 52 Months at Room Temperature RU45400
079 Plant RNA Broad Application of a Single Universal Lysis Buffer for True Total RNA Purification from Challenging Plant Species and Tissues 25800
080 Plant RNA Evaluation of Plant RNA Integrity Number (RIN) generated using an Agilent BioAnalyzer 2100 25800
081 Low Abundance DNA Quantification Comparing Different DNA and RNA Quantification Methods for Biological Samples with Low Nucleic Acid Abundance 57200*, 53100, 51300
082 DNA Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods 57300
083 RNA DNA Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device 46300, 54400, 48500
084 Plant Fungi DNA Detection of Genomic DNA from Seeds 26200
085 AAV Comparison of Stool DNA Preservation Methods and their Impact on Gut Microbiome Diversity 63100*
086 cf-DNA, cf-RNA Norgen's cf-DNA/cf-RNA Preservative Tube Preserves & Prevents the Contamination of Circulating Cell-Free DNA by Cellular Genomic DNA during Blood Collection, Shipping and Storage 63950, 55100
087 cf-DNA, cf-RNA Assessing the Effect of Simulated Shipping on Cell-Free DNA in Blood Collected in Norgen, Streck and EDTA Tubes 63950, 55100
088 Plant Fungi DNA RNA Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation 25800
090 Stool DNA Comparison of Stool DNA Preservation Methods and their Impact on Gut Microbiome Diversity 27600, 45630
091 Stool DNA Study of the Comparative Microbiome Profile from Different Fecal Preservation Methods 27600, 45630
092 Soil Plant Stool RNA Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve 25800, 27750, 49500

*Some of the kits mentioned within these Application Notes have evolved. If so, the current alternative has been linked to.