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Application Notes

The following Application Notes have been developed to offer additional resources to the researchers and scientists who use our kits. Norgen is pleased to share this knowledge with our fellow researchers, scientists, and collaborators.

Spanning a variety of topics and categories, these Notes were brought about by collaborations, customer inquiry, conference posters, as well as to make revisions to industry guidelines (ex. App Note 47 - Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input).

Our Notes share advances in technology, product observations and conclusions, proven validity and outcomes, and comparative education.

If you have questions about a product or service mentioned within an Application Note, contact us. We’re here to assist.


App Note Search



No. Category Application Note Related Kits
N/A RNA Effect of microRNA GC Content on RNA Purification Efficiency 17200, 23600, 29000, 42800
001 Protein Rapid Concentration of Dilute Protein Solutions Using the ProteoSpin™ CBED Kit 22800
002 Protein Rapid Desalting of Protein Solutions Using the ProteoSpin™ CBED Kit 22800
003 Protein Efficient Removal of Both Free and Tightly-Bound SDS from Protein Solutions using the ProteoSpin Detergent Clean-up Kit 22800
004 Protein Efficient Removal of Triton X-100 Prior to MALDI-TOF and Digestion Using the ProteoSpin Detergent Clean-up Kit 22800
005 Protein Screening for Bacterial Clones Expressing Inclusion Body Proteins 10300
006 Protein A Time Course Induction Study of Recombinant Protein Expression in E.coli 10300
008 DNA
Ladders		 DNA Quantification by Gel Densitometry with Norgen DNA Ladders 12800
009 RNA Isolation of Fractionated RNA from HeLa Cells Using Norgen's Cytoplasmic & Nuclear RNA Purification Kit 21000
010 DNA Isolation of DNA from as Little as 25 uL of Urine Using Norgen's Urine DNA Isolation kit 18100
011 RNA Isolation and Detection of RNA from as Little as a Single Animal Cell Using Norgen's Total RNA Purification Kit 17200
012 Multiple Analyte Sequential Isolation and Purification of RNA, DNA, and Protein from a Single Sample of Animal Cells using Norgen's RNA/DNA/Protein Purification Kit 47700
013 RNA Non-Organic-Based Isolation of Mammalian microRNA using Norgen's microRNA Purification Kit 21300
014 Plant RNA Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit 25800
015 Urine DNA Isolation of High Quality Urinary DNA using Norgen's Urine DNA Isolation Kit 18100
016 Plant microRNA Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit 25800
017 FFPE RNA Rapid Method for the Purification of Total RNA from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Samples 25300
018 RNA, DNA, Protein Sequential Purification of RNA, DNA, and Protein from a Single Sample using Norgen's RNA/DNA/Protein Purification Kit and Comparison to a Market Competitor 47700
019 Leukocyte RNA Rapid Isolation and Purification of Total Leukocyte (White Blood Cell) RNA from Mammalian Blood Samples 21200
020 RNA High-Throughput Purification of Total RNA Including miRNAwithout the use of Phenol Extraction 24300, 17200, 23600
021 Urine DNA Isolation of High Quality Urinary DNA Using Norgen's Urine DNA Mini Slurry Kit 48800
022 Total RNA Isolation of True Total RNA using the Tempus Blood RNA Tube and Norgen's Total RNA Purification Kit 17200
023 Saliva DNA Efficient Restriction Enzyme Digestion of Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, Isolation Kit 49000
024 Saliva DNA Long Term Stability of DNA Stored in Norgen's Saliva DNA Preservative 12100, 49000
025 Saliva DNA The Range of DNA Yield with Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 49000
026 Saliva DNA High Efficiency Real-Time PCR from Saliva DNA 49000
027 Saliva DNA Sex Determination from Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 49000
028 Saliva DNA Comparison of Norgen's Saliva DNA Collection, Preservation and Isolation Kit to a Leading Competitor's Kit 49000
029 Saliva DNA Sequencing Analysis of Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 49000, 14400
030 Saliva DNA Isolation of High Quality Saliva DNA from Variable Donor Saliva Samples 49000, 12100
031 Saliva DNA The Range of DNA Yield and Purity with Norgen's Saliva DNA Collection, Preservation, and Isolation Kit 49000
032 Saliva DNA Inhibitory Effect of Norgen's Saliva DNA Preservation on the Growth of Bacteria and Yeast 49000
038 Urine RNA Long Term Stability of RNA Stored in Norgen's Urine Preservation Buffer 18116
043 Saliva DNA Column-Based Isolation of DNA from Preserved Saliva Samples Offers High Quality, Consistent, and Rapid DNA Isolation 49000, RU45400
044 Saliva DNA DNA Isolation from Saliva Preserved using Norgen's Saliva DNA Collection and Preservation Device using Beckman Coulter Agencourt DNAdvance Kit RU49000
045 RNA Supplementary Protocol for Total RNA Isolation from Staphylococcus aureus-Related Bacteria and Hard-to-Lyse Bacteria Species 17200, 26533, 47700
046 DNA The Effect of Sample Preparation on Humic Acid Removal from Peat Samples: A Comparative Study 64000
047 RNA Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input 17200, 36200, 25300
048 Saliva DNA DNA Isolation from Saliva Preserved with Norgen's Saliva DNA Collection and Preservation Device using Qiagen's QIAmp DNA Blood Mini Kit RU45400, RU49000
049 RNA Effect of RNA Isolation Method on microRNA Quantity and Quality in Plasma: A Comparative Study 17200
050 Saliva DNA A Comparative Study Between Two Saliva DNA Preservation Systems, and Two Column-Based Saliva DNA Purification Methods RU45400, RU49000, 49000
051 Saliva DNA The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit RU49000
052 AAV DNA The Isolation of High Quality Stool DNA and its Application to Quantitative Adenovirus Detection using TaqMan® Real-Time PCR 27600, 45600, EP42600
053 DNA Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits 50600, 12100, 12800,
054 Saliva DNA Linearity of DNA Isolated from Increasing Volumes of Preserved Saliva Using Norgen’s Saliva DNA Isolation Kit RU45400, RU49000
055 Saliva DNA The Effect of Elution Volume on DNA Quantity and Quality Using Norgen’s Saliva DNA Isolation Kit RU45400, RU49000
056 Saliva DNA Comparative Study of DNA Isolated from Saliva Preserved in Norgen’s Preservative Using Norgen’s Saliva DNA Isolation Kit Versus Competitor’s DNA Blood Mini Kit RU45400, RU49000
057 Stool DNA Comparative Study of DNA Isolated from Stool Using Norgen’s Stool Nucleic Acid Isolation Kit and Norgen’s Stool DNA Isolation Midi Kit (Slurry Format) Versus Competitor’s DNA Stool Mini Kit 27600, 45600
058 Stool DNA Comparison of Stool DNA Isolation Methods for Bacterial and Mammalian DNA Detection 45600
059 Blood DNA Comparative Study of DNA Isolated from Increasing Volumes of Blood Using Norgen’s Blood Genomic DNA Isolation Kit Versus Qiagen’s QIAamp DNA Blood Mini Kit 46300
061 Plasma/Serum DNA Comparative Study of Plasma DNA Isolated Using Norgen’s Plasma/Serum Circulating DNA Purification Kits Versus Competitor’s DNA Blood Kits see Plasma/Serum Kits, 45600
063 Saliva DNA Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C RU49000, RU45400, 12100
064 Single Cell RNA Isolation of High Quality RNA from Single Mammalian Cell 51800
065 Blood DNA Genomic DNA Isolation from blood collected on different anticoagulants: Citrate, EDTA & Heparin 46300
066 Stool DNA Detection of Viral, Bacterial and Human Genomic DNA from Preserved Stool Samples 27600
067 Stool DNA Purification and Detection of Bacterial RNA from Preserved Stool Samples 27600
068 Plant DNA Detection of Genomic DNA from Processed Plant Oils and Wheat Flour 26200
069 Blood DNA Comparative Study of DNA Isolated from Whole Blood Using Norgen’s Blood Genomic DNA Isolation Mini Kit and Competitors’ Kits 46300
070 Blood DNA Genomic DNA Isolation from Buffy Coat using Norgen’s Blood Genomic DNA Isolation Mini Kit 46300
071 Blood DNA Genomic DNA Isolation from 1 µL – 100 µL of Whole Blood using Norgen’s Blood Genomic DNA Isolation Micro Kit 31200*
072 Blood DNA Effect of Elution Volume on DNA Recovery and Quality using Norgen’s Blood Genomic DNA Isolation Micro Kit 31200*
073 Blood DNA Single Elution vs. Re-Elution: Implications on Concentration and Purity using the Blood Genomic DNA Isolation Micro Kit 31200*
074 Blood DNA Isolation of Genomic DNA from Dried Blood Collected on Different Materials: Blood Cards, Cloth and Solid Surface using Norgen’s Dried Blood Spot Genomic DNA Isolation Kit 36000*
075 Blood DNA Isolation of DNA from Hair Follicles and Hair Shafts using Norgen’s Dried Blood Spot Genomic DNA Isolation Kit 36000*
076 Cells and Tissue DNA Isolation of DNA from 0.1 – 3 million HeLa Cells using Norgen’s Cell and Tissue Genomic DNA Isolation Kit 53100
077 Soil DNA Norgen’s Soil DNA Isolation Kit Outperforms a Competitor with Effective Humic Acid Removal for Metagenomic Studies 64000
078 Saliva DNA Stability of DNA Stored in Norgen’s Saliva DNA Preservative for 52 Months at Room Temperature RU49000, RU45400
079 Plant RNA Broad Application of a Single Universal Lysis Buffer for True Total RNA Purification from Challenging Plant Species and Tissues 25800
080 Plant RNA Evaluation of Plant RNA Integrity Number (RIN) generated using an Agilent BioAnalyzer 2100 25800
081 Low Abundance DNA Quantification Comparing Different DNA and RNA Quantification Methods for Biological Samples with Low Nucleic Acid Abundance 57200*, 53100, 51300
082 DNA Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods 57300
083 RNA DNA Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device 46300, 54400, 48500
084 Plant Fungi DNA Detection of Genomic DNA from Seeds 26200
085 AAV Purification of Adeno-associated Virus (AAV) Vectors Using Norgen's AAV Mini and Midi Purification Kits 63100*
086 cf-DNA, cf-RNA How to Preserve Cell-Free DNA and Prevent Cellular Genomic DNA Contamination in Plasma 63950, Dx63950, 55100, 55500, 55600, 55800
087 cf-DNA, cf-RNA Assessing the Effect of Simulated Shipping on Cell-Free DNA in Blood Collected in Norgen, Streck and EDTA Tubes 63950, 55100
088 Plant Fungi DNA RNA Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation 25800
091 Stool DNA Study of the Comparative Microbiome Profile from Different Fecal Preservation Methods 27600, 45630
092 Soil Plant Stool RNA Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve 25800, 27750, 49500
093 Saliva RNA Viral Inactivation Using Norgen’s Total Nucleic Acid Preservative and Norgen’s Saliva RNA Preservative Dx69200, 69200, 53800
094 Saliva RNA Inactivation of Coronavirus in Samples using Norgen's Saliva RNA Preservative Dx69200, 69200, 53800, RU53800
095A Total RNA Comparison of Norgen’s Saliva/Swab RNA Purification Kit to a Magnetic Bead-Based Competitor's Kit for Viral RNA Extraction Dx69100, 69100, Dx69200, 69200, DxTM67200, TM67200
095B Total RNA Comparison of Norgen’s Saliva/Swab RNA Purification Kit to Zymo’s MagBinding Quick-Viral DNA/RNA kit for Viral RNA Extraction Dx69100, 69100, Dx69300, 69300, DxTM67200, TM67200
096 Stool DNA RNA Immediate Viral Inactivation Using Norgen’s Stool Nucleic Acid Preservative 45660, Dx45660, 63700, 27600, 65600, 45600

*Some of the kits mentioned within these Application Notes have evolved. If so, the current alternative has been linked to.