Resources

The following Application Notes have been developed to offer additional resources to the researchers and scientists who use our kits. Norgen is pleased to share this knowledge with our fellow researchers, scientists, and collaborators.

Spanning a variety of topics and categories, these Notes were brought about by collaborations, customer inquiry, conference posters, as well as to make revisions to industry guidelines (ex. App Note 47 - Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input).

Our Notes share advances in technology, product observations and conclusions, proven validity and outcomes, and comparative education.

If you have questions about a product or service mentioned within an Application Note, contact us. We’re here to assist.

App Note Search

No.	Category	Application Note	Related Kits
001	Protein	Rapid Concentration of Dilute Protein Solutions Using the ProteoSpin™ CBED Kit	22800
002	Protein	Rapid Desalting of Protein Solutions Using the ProteoSpin™ CBED Kit	22800
003	Protein	Efficient Removal of Both Free and Tightly-Bound SDS from Protein Solutions using the ProteoSpin Detergent Clean-up Kit	22800
004	Protein	Efficient Removal of Triton X-100 Prior to MALDI-TOF and Digestion Using the ProteoSpin Detergent Clean-up Kit	22800
005	Protein	Screening for Bacterial Clones Expressing Inclusion Body Proteins	10300
006	Protein	A Time Course Induction Study of Recombinant Protein Expression in E.coli	10300
008	DNA Ladders	DNA Quantification by Gel Densitometry with Norgen DNA Ladders	12800
009	RNA	Isolation of Fractionated RNA from HeLa Cells Using Norgen's Cytoplasmic & Nuclear RNA Purification Kit	21000
010	DNA	Isolation of DNA from as Little as 25 uL of Urine Using Norgen's Urine DNA Isolation kit	18100
011	RNA	Isolation and Detection of RNA from as Little as a Single Animal Cell Using Norgen's Total RNA Purification Kit	17200
012	Multiple Analyte	Sequential Isolation and Purification of RNA, DNA, and Protein from a Single Sample of Animal Cells using Norgen's RNA/DNA/Protein Purification Kit	47700
013	RNA	Isolation and Detection of RNA from as Little as a Single Animal Cell Using Norgen's Total RNA Purification Kit	21300
014	Plant RNA	Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit	25800
015	Urine DNA	Isolation of High Quality Urinary DNA using Norgen's Urine DNA Isolation Kit	18100
016	Plant microRNA	Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit	25800
017	FFPE RNA	Rapid Method for the Purification of Total RNA from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Samples	25300
018	RNA, DNA, Protein	Sequential Purification of RNA, DNA, and Protein from a Single Sample using Norgen's RNA/DNA/Protein Purification Kit and Comparison to a Market Competitor	47700
019	Leukocyte RNA	Rapid Isolation and Purification of Total Leukocyte (White Blood Cell) RNA from Mammalian Blood Samples	21200
020	RNA	High-Throughput Purification of Total RNA Including miRNA without the use of Phenol	24300
021	Urine DNA	Isolation of High Quality Urinary DNA Using Norgen's Urine DNA Mini Slurry Kit	48800
022	Total RNA	Isolation of True Total RNA using the Tempus Blood RNA Tube and Norgen's Total RNA Purification Kit	17200
023	Saliva DNA	Efficient Restriction Enzyme Digestion of Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, Isolation Kit	35700
024	Saliva DNA	Long Term Stability of DNA Stored in Norgen's Saliva DNA Preservative	12100, 35700
025	Saliva DNA	The Range of DNA Yield with Norgen's Saliva DNA Collection, Preservation, and Isolation Kit	35700
026	Saliva DNA	High Efficiency Real-Time PCR from Saliva DNA	35700
027	Saliva DNA	Sex Determination from Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, and Isolation Kit	35700
028	Saliva DNA	Comparison of Norgen's Saliva DNA Collection, Preservation and Isolation Kit to a Leading Competitor's Kit	35700
029	Saliva DNA	Sequencing Analysis of Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, and Isolation Kit	35700 14400
030	Saliva DNA	Isolation of High Quality Saliva DNA from Variable Donor Saliva Samples	35700, 12100
031	Saliva DNA	The Range of DNA Yield and Purity with Norgen's Saliva DNA Collection, Preservation, and Isolation Kit	35700
032	Saliva DNA	Inhibitory Effect of Norgen's Saliva DNA Preservation on the Growth of Bacteria and Yeast	35700
033	Urine DNA	The Range of DNA Yield and Purity with Norgen's Urine DNA Concentration, Preservation, and Isolation Kit	see Urine Products*
034	Urine DNA	High Efficiency Real-Time PCR from Urine DNA	see Urine Products*
035	Urine DNA	Efficient Restriction Enzyme Digestion of Urine DNA Isolated using Norgen's Urine DNA Concentration, Preservation, and Isolation Kit	see Urine Products*, 12800
036	Urine RNA	The Range of RNA Yield and Purity with Norgen's Urine RNA Concentration, Preservation, and Isolation Kit	see Urine Products*
037	Urine RNA	The Range of RNA Yield and Purity with Norgen's Urine RNA Concentration, Preservation, and Isolation Kit	see Urine Products*
038	Urine RNA	Long Term Stability of RNA Stored in Norgen's Urine Preservation Buffer	18116
039	Urine Protein	The Range of Protein Yield with Norgen's Urine Protein Concentration, Preservation, and Isolation Kit	see Urine Products
041	Urine DNA	The Range of DNA Yield and Purity with Norgen's Urine DNA Concentration, Preservation, and Isolation Kit (For Resource Limited Settings)	see Urine Products
042	Urine RNA	The Range of RNA Yield and Purity with Norgen's Urine DNA Concentration, Preservation, and Isolation Kit (For Resource Limited Settings)	see Urine Products
043	Saliva DNA	Column-Based Isolation of DNA from Preserved Saliva Samples Offers High Quality, Consistent, and Rapid DNA Isolation	35700, RU45400
044	Saliva DNA	DNA Isolation from Saliva Preserved using Norgen's Saliva DNA Collection and Preservation Device using Beckman Coulter Agencourt DNAdvance Kit	RU45400, RU35700, RU49000
045	RNA	Supplementary Protocol for Total RNA Isolation from Staphylococcus aureus-Related Bacteria and Hard-to-Lyse Bacteria Species	17200, 26533, 47700
046	DNA	The Effect of Sample Preparation on Humic Acid Removal from Peat Samples: A Comparative Study	64000
047	RNA	Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input	17200, 36200, 25300
048	Saliva DNA	DNA Isolation from Saliva Preserved with Norgen's Saliva DNA Collection and Preservation Device using Qiagen's QIAmp DNA Blood Mini Kit	RU45400, RU35700, RU49000
049	RNA	Effect of RNA Isolation Method on microRNA Quantity and Quality in Plasma: A Comparative Study	17200
050	RNA	A Comparative Study Between Two Saliva DNA Preservation Systems, and Two Column-Based Saliva DNA Purification Methodsy	RU45400
051	Saliva DNA	The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit	35700
052	AAV DNA	The Isolation of High Quality Stool DNA and its Application to Quantitative Adenovirus Detection using TaqMan® Real-Time PCR	27600, 45600, EP42600
053	DNA	Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits	50600, 12100, 12800,
054	Saliva DNA	Linearity of DNA Isolated from Increasing Volumes of Preserved Saliva Using Norgen’s Saliva DNA Isolation Kit	RU45400, RU35700, RU49000
055	Saliva DNA	The Effect of Elution Volume on DNA Quantity and Quality Using Norgen’s Saliva DNA Isolation Kit	RU45400, RU35700, RU49000
056	Saliva DNA	Comparative Study of DNA Isolated from Saliva Preserved in Norgen’s Preservative Using Norgen’s Saliva DNA Isolation Kit Versus Competitor’s DNA Blood Mini Kit	RU45400, RU35700, RU49000
057	Stool DNA	Comparative Study of DNA Isolated from Stool Using Norgen’s Stool Nucleic Acid Isolation Kit and Norgen’s Stool DNA Isolation Midi Kit (Slurry Format) Versus Competitor’s DNA Stool Mini Kit	27600, 45600
058	Stool DNA	Comparison of Stool DNA Isolation Methods for Bacterial and Mammalian DNA Detection	45600
059	Blood DNA	Comparative Study of DNA Isolated from Increasing Volumes of Blood Using Norgen’s Blood Genomic DNA Isolation Kit Versus Competitor’s DNA Blood Mini Kit	46300
060	Stool DNA	DNA Isolation from 1 Gram of Stool Using a Silicon Carbide Slurry Method: Comparison to 250mg of Stool and Identification of RNA Presence in Low Molecular Weight DNA Fraction	45600
061	Plasma/Serum DNA	Comparative Study of Plasma DNA Isolated Using Norgen’s Plasma/Serum Circulating DNA Purification Kits Versus Competitor’s DNA Blood Kits	see Plasma/Serum Kits, 45600
062	Saliva DNA	Stability of DNA Stored in Norgen’s Saliva DNA Preservative for Over 30 Months	RU45400, 12100
063	Saliva DNA	Stability of DNA Stored in Norgen’s Saliva DNAPreservative at 55°C	RU45400, 12100
064	Single Cell RNA	Isolation of High Quality RNA from Single Mammalian Cell	51800
065	Blood DNA	Genomic DNA Isolation from blood collected on different anticoagulants: Citrate, EDTA & Heparin	46300
066	Stool DNA	Detection of Viral, Bacterial and Human Genomic DNA from Preserved Stool Samples	27600
067	Stool DNA	Purification and Detection of Bacterial RNA from Preserved Stool Samples	27600
068	Plant DNA	Detection of Genomic DNA from Processed Plant Oils and Wheat Flour	26200
069	Blood DNA	Comparative Study of DNA Isolated from Whole Blood Using Norgen’s Blood Genomic DNA Isolation Mini Kit and Competitors’ Kits	46300
070	Blood DNA	Genomic DNA Isolation from Buffy Coat using Norgen’s Blood Genomic DNA Isolation Mini Kit	46300
071	Blood DNA	Genomic DNA Isolation from 1 µL – 100 µL of Whole Blood using Norgen’s Blood Genomic DNA Isolation Micro Kit	31200*
072	Blood DNA	Effect of Elution Volume on DNA Recovery and Quality using Norgen’s Blood Genomic DNA Isolation Micro Kit	31200*
073	Blood DNA	Single Elution vs. Re-Elution: Implications on Concentration and Purity using the Blood Genomic DNA Isolation Micro Kit	31200*
074	Blood DNA	Isolation of Genomic DNA from Dried Blood Collected on Different Materials: Blood Cards, Cloth and Solid Surface using Norgen’s Dried Blood Spot Genomic DNA Isolation Kit	36000*
075	Blood DNA	Isolation of DNA from Hair Follicles and Hair Shafts using Norgen’s Dried Blood Spot Genomic DNA Isolation Kit	36000*
076	Cells and Tissue DNA	Isolation of DNA from 0.1 – 3 million HeLa Cells using Norgen’s Cell and Tissue Genomic DNA Isolation Kit	53100
077	Soil DNA	Norgen’s Soil DNA Isolation Kit Outperforms a Competitor with Effective Humic Acid Removal for Metagenomic Studies	64000
078	Saliva DNA	Stability of DNA Stored in Norgen’s Saliva DNA Preservative for 52 Months at Room Temperature	RU45400
079	Plant RNA	Broad Application of a Single Universal Lysis Buffer for True Total RNA Purification from Challenging Plant Species and Tissues	25800
080	Plant RNA	Evaluation of Plant RNA Integrity Number (RIN) generated using an Agilent BioAnalyzer 2100	25800
081	Low Abundance DNA Quantification	Comparing Different DNA and RNA Quantification Methods for Biological Samples with Low Nucleic Acid Abundance	57200*, 53100, 51300
082	DNA	Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods	57300
083	RNA DNA	Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device	46300, 54400, 48500
084	Plant Fungi DNA	Detection of Genomic DNA from Seeds	26200
085	AAV	Comparison of Stool DNA Preservation Methods and their Impact on Gut Microbiome Diversity	63100*
086	cf-DNA, cf-RNA	Norgen's cf-DNA/cf-RNA Preservative Tube Preserves & Prevents the Contamination of Circulating Cell-Free DNA by Cellular Genomic DNA during Blood Collection, Shipping and Storage	63950, 55100
087	cf-DNA, cf-RNA	Assessing the Effect of Simulated Shipping on Cell-Free DNA in Blood Collected in Norgen, Streck and EDTA Tubes	63950, 55100
088	Plant Fungi DNA RNA	Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation	25800
090	Stool DNA	Comparison of Stool DNA Preservation Methods and their Impact on Gut Microbiome Diversity	27600, 45630
091	Stool DNA	Study of the Comparative Microbiome Profile from Different Fecal Preservation Methods	27600, 45630
092	Soil Plant Stool RNA	Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve	25800, 27750, 49500

Norgen's Complete Workflow

Norgen is a complete workflow provider for your RNA/microRNA/DNA needs - from sample collection, preservation, and purification to NGS. We have particular expertise in ultra-low input samples including those from liquid biopsies, exosomes, and more.