Supporting Data

Previous

Figure 1.  Example of TaqMan One-step RT-PCR Positive result. PCR signals above the baseline from FAM, Tex615, Cy5, and HEX channel indicate the successful PCR. Individual amplification in any of the channels also indicates a positive result for the pathogen corresponding to that channel.

Figure 2.  Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM,TEX615, and Cy5 channels but amplification signal from HEX indicates the successful PCR.

Figure 3.  Example of TaqMan one-step RT-PCR inhibition result. No signal from all targets was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

1. 
2. 
3. 

Next

Click for expanded view

Component	Cat. TM69950 (100 preps)	Cat. TM69910 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
Mastitis Pathogens Primer & Probe Mix	280 μL	280 μL
Mastitis pathogens Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Documentation

PROTOCOLS

(TM65950) Mastitis TaqMan RT-PCR Kit - Protocol (100 rxns)

---

(TM65910) Mastitis TaqMan Probe/Primer and Control Set - Protocol (100 rxns)