Features and Benefits

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Ten discrete fragments from 100 bp to 1000 bp
• Higher intensity reference band at 500 bp

The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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Figure 1:  PCR Sizer 100 bp DNA Ladder

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PCR Sizer 100 bp DNA Ladder (Cat# 11400) - 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

 

Fragment	Size (bp)	Mass (ng)
1	1000	78
2	900	70
3	800	62
4	700	55
5	600	47
6	500	78
7	400	31
8	300	24
9	200	31
10	100	24

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

 

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

PROTOCOLS

11400 - Product Insert

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders

SAFETY DATA SHEETS

11400 - PCR Sizer 100 BP DNA Ladder - SDS

Citations

Title	Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers
Citation	Genetics and Molecular Research 2013.
Authors	D Molin, CJ Coelho, DS Maximo, FS Ferreira, JR Gardingo, RR Matiello