Name: Plasma/Serum Total cfc-Nucleic Acid Advanced Purification Kit
Brand: Norgen Biotek Corp.
SKU: 68100
Price: 1045.000000 USD
Availability: InStock

Supporting Data

Figure 1. A representative Bioanalyzer RNA Pico Chip trace showing the quality/quantity of the RNA purified from 4 mL K2 EDTA plasma using A) Norgen’s Plasma/Serum Total cfc-Nucleic Acid Advanced Purification Kit (Automated Isolation), B) Norgen’s Plasma/Serum Total cfc-Nucleic Acid Advanced Purification Kit (Manual Isolation), C) Competitor Q's ccfDNA/RNA Kit and D) Competitor Q's Circulating Nucleic Acid Kit. All purification methods showed the correct cf-RNA size on the bioanalyzer trace however the amount of the cf-RNA recovered using Norgen’s technology (as can be noticed by the height of the cf-RNA peak) was significantly higher than that for the cf-RNA purified using the silica-based technology.

Figure 2. A representative Bioanalyzer High Sensitivity DNA Chip trace showing the quality/quantity of the cf-DNA purified from 4 mL K2 EDTA plasma A) Norgen’s Plasma/Serum Total cfc-NA Advanced Purification Kit (Automated Isolation), B) Norgen’s Plasma/Serum Total cfc-NA Advanced Purification Kit (Manual Isolation), C) Competitor Q's ccfDNA/RNA Kit and D) Competitor Q's MinElute ccfDNA Midi Kit. All purification methods showed the correct cf-DNA size (~180bp) on the bioanalyzer trace however the amount of the cf-DNA recovered using Norgen’s technology (as can be noticed by the height of the cf-DNA peak) was significantly higher than recovered using the silica-based technology.

Figure 3. Real-Time PCR amplification of miR-21, miR-16 (cfc-RNA targets) and ALU 247, ALU 115 (cfc-DNA targets) from Total cfc-Nucleic acid purified from 4mL K2EDTA plasma using different purification methods.. Norgen’s SiC technology, manual and automated procedure, was superior as compared to the amplification of the same targets amplified from Total Nucleic aicd purified from K2EDTA plasma using the Silica-based technology represented by Competitor Q.

Kit Specifications
Minimum Plasma/Serum Input	1 mL
Maximum Plasma/Serum Input	6 mL
Size of cfc-RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Optimal size of purified cfc-DNA	146 - 180 bp
Elution Volume	25-50 µL
Time to Complete 10 Purifications	45 minutes
Average Yields*	Variable depending on specimen

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Components	Cat. 68100 (50 preps)
Binding Buffer A	20 mL
Proteinase K	6.5 mL
Slurry E	12.5 mL
Lysis Buffer A	130 mL
Wash Solution A	38 mL
Elution Buffer C	2 x 8 mL
Elution Buffer F	15 mL
Elution Solution A	20 mL
Micro Spin Columns	50
Mini Filter Spin Columns	50
Collection Tubes	100
Elution Tubes (1.7 mL)	100
Product Insert	1

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen’s Plasma/Serum CFC-RNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Plasma/Serum Total cfc-Nucleic Acid Advanced Purification Kit