从小输入量中快速纯化总 RNA（包括 microRNA）
细节
Supporting Data
Figure 1. Better Efficiency of RNA Recovery at Single Cell Input.
Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor’s kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit and a competitor’s spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript in the isolated RNA. RNA isolated by Norgen’s Single Cell RNA Purification Kit showed much higher relative expression of each RNA transcript tested at all cell input numbers down to single cell.
Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM).
Norgen’s Single Cell RNA Purification Kit allows sensitive but high-quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen’s Single Cell RNA Purification Kit recovered good amounts of RNA with an excellent 260/280 ratio.
Figure 3. Consistent and Linear Recovery of All Sizes of RNA down to Single Cell Input.
Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell with great linearity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit. The extracted RNA was subjected to RT-qPCR to detect all sizes of RNA, including the human S15 (mRNA), 5S rRNA transcript (small RNA), and miR-21 (microRNA). RNA isolated by Norgen’s Single Cell RNA Purification Kit showed very high consistency (over 99%) of recovery of each RNA transcript tested at all cell input numbers down to a single cell.
支持数据
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试剂盒规格
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色谱柱最大结合能力
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10 μg
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最大色谱柱装载量
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650 μL
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最小洗脱体积
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8 μL |
| 纯化的 RNA 大小 |
所有大小，包括小 RNA（<200 nt）
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起始材料的最大用量： 动物细胞 激光捕获显微切割 (LCM) |
单细胞到 a 2 x 105 个细胞 多达 2 x 105 个细胞 |
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完成 10 次净化所需的时间
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20 分钟
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平均产量： HeLa 细胞 (1 x 105 个细胞) HeLa 细胞 (100 个细胞) HeLa 细胞 (1 个细胞) |
~ 1.5 µg ~ 1.5 ng ≥ 1pg |
储存条件和产品稳定性
所有溶液都应密封保存在室温下。该试剂盒自发货之日起 2 年内保持稳定。