用于简单、非侵入性地采集唾液并在室温下保存唾液样本中的 DNA
细节
Supporting Data
Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for up to 75 Months.
Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 75 months. Saliva DNA was subsequently isolated at 1 week, 3 weeks, 1 month, 2 months, 4 months, 6 months, 8 months, 12 months, 20 months, 32 months, 40 months, 52 months and 75 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 75 months at ambient temperatures in Norgen's Saliva DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 75-month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).
Figure 2. GAPDH Real Time PCR Amplification of Saliva DNA from Saliva Samples Stored at Room Temperature from Day 0 up to 24 Months.
All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition and difference in Ct. Red: Day 0, Blue: 4 months, Purple: 8 months, Green: 16 months and Sky blue: 24 months. Black: No template control.
Figure 3. V3-V4 16s rRNA PCR amplification for Illumina MiSeq 16s rRNA library preparation.
The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 2 years at room temperature in Norgen’s Saliva DNA Preservative. The PCR result shows the clean PCR amplification at ~550 bp indicating the right target of V3-V4 16s rRNA , with no difference between day 0 and 24 months. M: FastRunner DNA Ladder (Cat. 12800).
支持数据
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E试剂盒规格
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采集的唾液量
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2 mL
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唾液-保存剂混合液体积
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4 mL
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保存温度
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室温
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| 保存时间 |
室温下 2 年以上
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保质期和处理
- 在室温下储存时，未使用的 Norgen 唾液保存剂试管可稳定地保存至保质期，其性能不会降低。请参阅设备说明书或试管标签上的保质期。
- 唾液采集后，如果密封并在室温下保存，可稳定保存 2 年以上。
- 每个唾液 DNA 采集和保存装置的采集管、采集漏斗和装置容器均可回收。