用于快速、轻松地收集和保存尿液样本
尿液采集和保存设备
用于快速、轻松地收集和保存尿液样本
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概述
Norgen 的尿液保存专为快速保存新鲜尿液样本中的 DNA、RNA、microRNA 和蛋白质而设计。尿液保存可防止革兰氏阴性和革兰氏阳性细菌和真菌的生长,还能灭活病毒,从而使非感染性样本得以安全处理和运输。此外,尿液保存剂无需立即处理或冷冻样本,可在环境温度下将样本运送到集中检测设施。尿液保存的成分可使样本在室温下保存 2 年以上,且检测不到尿液 DNA、RNA 或蛋白质的降解。
Norgen 的尿液保存有两种方便形式:
1. 尿液保存单剂量安瓿
Norgen 的尿液保存以液体形式在单剂量安瓿中供应。用户只需在尿液采集容器中采集 5 - 50 mL 尿液,然后加入单剂量尿液保存(类别号:18126)。然后将尿液和保存混合,在室温下保存尿液核酸和蛋白质。
2. 尿液采集和保存剂试管
Norgen 的尿液保存还可在 Norgen 的尿液采集和保存剂试管中以干燥形式提供。用户只需将尿液采集到试管中,然后轻轻搅拌,直至试管中的橙色保存颗粒溶解。Norgen 的尿液采集和保存剂试管有 3 种方便尺寸:
- 5 cc 试管, 1 - 5 mL 尿液
- 15 cc 试管, 5 - 15 mL 尿液
- 50 cc 试管, 5 - 45 mL 尿液
- 120 cc 杯, 100 - 120 mL 尿液
细节
Supporting Data
Figure 1. Stability of DNA in Norgen's Urine Stabilizer.
Urine samples were collected from numerous healthy donors and pooled, and then the urine was aliquoted into 5 mL aliquots in Norgen's Urine Collection and Stabilization Tubes (5 cc) (Cat# 18116). The stabilized urine was stored at -20°C, 4°C and room temperature for up to 48 months. Urine DNA was subsequently isolated at time 0, 1 month, 2 months, 4 months, 8 months, 12 months, 16 months, 20 months and 24 months from the stabilized urine using Norgen's Urine DNA Isolation Kit (Slurry Format) (Cat# 48800). To test the integrity of the purified DNA, 3 μL of each elution was used in a 20 μL Taqman PCR reaction run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using Norgens 2X PCR Master Mix with 5s rRNA primers. As it can be seen, all the DNA samples were successfully amplified.
Figure 2. Stability of RNA in Norgen's Urine Stabilizer.
Urine samples were collected from numerous healthy donors and pooled, and then the urine was aliquoted into 5 mL aliquots in Norgen's Urine Collection and Stabilization Tubes (5 cc) (Cat. 18116). The stabilized urine was stored at -20°C, 4°C, and room temperature for up to 24 months. Urine RNA was then subsequently isolated at time 0, 1 month, 2 months, 4 months, 8 months, 12 months, 16 months, and 24 months from the preserved urine using Norgen's Urine Total RNA Purification Maxi Kit (Slurry Format) (Cat. 29600).
使用 Norgen 尿液采集和保存剂试管检测的精选尿液
鼠标
山猫
狼
从雪中采集的尿液
可用尺寸和形式
- 尿液采集和保存剂试管 (50 cc) - 1-5 mL 的尿液输入量
储存条件和产品稳定性
所有试管均应密封保存,并在室温(15 - 25°C)条件下存放长达 1 年之久,其性能不会降低。
Documentation
- in a timely manner that considers the person's accessibility needs due to disability; and
- at a cost that is no more than the regular cost charged to other persons