快速、简单的粪便样本采集、保存和发送方法
概述
Norgen 的粪便拭子采集和保存系统专为采集、常温储存和运输粪便标本中的核酸而设计。粪便拭子采集和保存系统试管中含有液体形式的 Norgen 粪便保存剂。用户只需使用提供的拭子采集样本，然后将拭子移入粪便保存剂中即可。粪便保存剂可防止革兰氏阴性和革兰氏阳性细菌和真菌的生长，还能灭活病毒，从而使非感染性样本得到安全处理和运输。此外，粪便保存剂无需立即处理或冷冻样本，可在环境温度下将样本运送到集中检测设施。粪便保存剂的成分可使样本在室温下保存 2 年以上 (DNA) 和 7 天 (RNA)。为延长粪便 RNA 在保存剂中的稳定性，建议在运抵检测机构后将其保存在 -20°C 或 -70°C 温度下，直至 RNA 纯化。
细节
Supporting Data
Figure 9. Norgen's Best-In-Class Preservation.
Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods.
Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples.
DNA and RNA were isolated from 200 µL of preserved stool from 4 different donors (lanes A to D) stored at room temperature at the indicated time points (DNA at 27 months and RNA at 7 days) using Norgen's Stool Nucleic Acid Isolation Kit (Cat# 45600). Next, 10 µL from the 75 µL eluted nucleic acid was loaded onto a gel for visual analysis. High integrity stool DNA was isolated from preserved stool samples that were stored at room temperature for 27 months, and high integrity RNA was isolated from stool samples stored for 7 days at ambient temperature. Lane M is Norgen's HighRanger 1 kb DNA Ladder (Cat. 11900).
Figure 3. Monitoring the DNA stability of Adenovirus (Ad5subE1) Spiked into Preserved Stool Samples using TaqMan Real-Time PCR Assay.
Preserved stool samples were spiked with 6 x 109 pfu/mL of Adenovirus, and 200 µL samples were removed at the indicated time points. The stool DNA was then isolated using Norgen's Stool DNA Isolation Kit (Cat# 27600). Next, 2 µL of DNA template from each 50 µL of elution was used in a 20 µL PCR reaction to detect the Adenovirus DNA. The stool preservative was shown to stabilize Adenovirus DNA up to 21 weeks at ambient temperature, indicating that Norgen's Stool Nucleic Acid Collection and Preservation Tubes provide a safe and convenient sample transportation system.
Figure 4. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions.
The microbial composition from the stool samples preserved in Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period while non-preserved stool samples showed a distance in the dendrogram from the original stool profile.
Figure 5. Difference In Top 5 Abundant Microorganisms at Phylum Level Compared to Fresh Stool (Day 0).
Abundance of Firmicuties, Bacteroidetes and proteobacteria from the preserved stool sample was higher than non-preserved (NP) to the fresh stool after 2 days storage at RT, indicating that the originality of microorganism profile from the preserved are much closer to the fresh stool sample (Day 0).
Figure 6. Relative Abundance of Bacterial Genera Between Preserved And Non-preserved Stool Sample Stored at RT For 2 Days.
Preserved stool sample using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) showed closed relativeness to the fresh stool sample (Day 0).
Figure 7. Correlation between duplicate bacterial metagenomic reads.
Correlation between duplicate bacterial metagenomic reads using (A) Fresh stool (day 0) and preserved stool at RT for 2 days and (B) Fresh stool (day 0) and non-preserved stool at RT for 2 days. A greater R2 was observed for Fresh stool (day 0) and preserved stool, suggestive of higher consistency between fresh stool sample and preserved stool sample
支持数据
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试剂盒规格
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| 粪便核酸在室温下的稳定性 |
DNA 为 2 年，RNA 长达 7 天*。
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* RNA 的稳定性因样本而异
储存条件和产品稳定性
粪便拭子采集和保存系统（散装形式）可在室温下保存 2 年，而不会降低试剂盒的性能。棉签的有效期写在棉签包装纸上。