概述

Norgen 的拭子采集和 DNA 保存系统设计用于从使用拭子采集的样本（包括鼻腔、口腔、唾液、粪便、皮肤、表面等）中采集、常温保存和运输 DNA。拭子采集和 DNA 保存系统包含 Norgen 液体形式的拭子保存剂。用户只需使用提供的拭子采集样本，然后将拭子转移到拭子保存剂中。拭子保存剂可防止革兰氏阴性和革兰氏阳性细菌和真菌的生长，还能灭活病毒，从而使非感染性样本得到安全处理和运输。此外，拭子保存剂无需立即处理或冷冻样本，可在环境温度下将样本运送到集中检测设施。拭子保存剂的成分可使 DNA 样本在室温下保存 2 年以上。

从保存剂中分离 DNA

使用 Norgen 的拭子采集和 DNA 保存系统采集和保存的拭子样本与大多数 DNA 分离方法兼容。储存在试管中的样本已成功用于 Norgen Biotek 的大多数 DNA 分离试剂盒和试剂。经过优化的分离程序可作为 Norgen 微生物组 DNA 分离试剂盒（类别号：64100）。

细节

Supporting Data

Figure 1 / 4

High Quality Preserved DNA — Figure 1. Detection of GAPDH Using Taqman Real-time PCR from the Saliva Samples Stored at Room Temperature from 1 Week Up to 75 Months.

The quality of the saliva DNA stored at room temperature did not change from 1 week to 75 months, as indicated by the fact that the Ct values remained consistent. The improvement in the Ct values from the real-time PCR graph (red) also indicate the impact of the improved saliva DNA isolation method provided by the Saliva DNA Isolation Kit (Cat. RU45400).

PCR Amplification of Preserved DNA — Figure 2. Real Time PCR Amplification of Saliva DNA from the saliva samples stored at room temperature from 1 week up to 75 months (13 time points).

All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition. Blue: Saliva DNA isolated using improved Saliva DNA Isolation Kit (Cat. RU45400). Black: No template control.

Figure 3. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Swab DNA Preservative.

The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.

Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for up to 75 Months.

Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 75 months. Saliva DNA was subsequently isolated at 1 week, 3 weeks, 1 month, 2 months, 4 months, 6 months, 8 months, 12 months, 20 months, 32 months, 40 months, 52 months and 75 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 75 months at ambient temperatures in Norgen's Saliva DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 75-month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).

支持数据

图 1.诺健拭子 DNA 保存剂中保存的 DNA 在室温下的稳定性长达 75 个月。从众多捐献者处采集唾液样本并混合，然后在唾液中加入等量的 Norgen 的拭子 DNA 保存剂。保存后的唾液在室温下可保存 75 个月。随后，在 1 周、3 周、1 个月、2 个月、4 个月、6 个月、8 个月、12 个月、20 个月、32 个月、40 个月、52 个月和 75 个月时，使用 Norgen 的唾液 DNA 分离试剂盒（类别号：RU45400）从 0.5 mL 唾液/保存剂样本中分离唾液 DNA。为了进行直观分析，将 100 µL 纯化的 DNA 在琼脂糖 TAE 凝胶上运行。可以看出，唾液样本在环境温度下在 Norgen 拭子 DNA 保存剂中保存 75 个月后，DNA 没有降解迹象。此外，在整个 75 个月期间，DNA 的大小一直保持在 24 kb 以上。M：Norgen 的 UltraRanger 1 Kb DNA Ladder（类别号：12100）。

图 2.使用 TaqMan 实时聚合酶链式反应检测室温保存 1 周至 75 个月的唾液样本中的 GAPDH。在室温下储存的唾液 DNA 的质量从 1 周到 75 个月都没有发生变化，这体现在 Ct 值保持一致。实时聚合酶链式反应图（红色）中 Ct 值的改善也表明了唾液 DNA 分离试剂盒（类别号：RU45400）从 0.5 mL 唾液/保存剂样本中分离唾液 DNA。

图 3.对室温下保存 1 周至 75 个月（13 个时间点）的唾液样本进行唾液 DNA 的实时聚合酶链式反应扩增。所有分离的唾液 DNA 样本都成功扩增，没有出现任何聚合酶链式反应抑制迹象。蓝色：使用改进的唾液 DNA 分离试剂盒（类别号：RU45400）从 0.5 mL 唾液/保存剂样本中分离唾液 DNA。黑色：无模板控制。

图 4.使用 Norgen Swab DNA 保存剂保存超过 6 年的唾液样本中的 Illumina MiSeq 16s rRNA 元基因组学数据。唾液 DNA 使用 Norgen 唾液 DNA 分离试剂盒（类别号：RU45400）的唾液，这些唾液在室温下保存了不同的时间，最长可达 6 年。A) 对 12 个唾液微生物组进行的主坐标分析显示，不同样本之间的分类分布存在差异，最高可达王国级别。B) 根据属级分类对 12 个唾液微生物组进行分级聚类，包括显示树枝图中每个样本属级分类相对丰度的柱状图。

试剂盒规格
保保存剂体积	1.2 mL
保存温度	室温
保存时间	室温下 2 年

保质期和处理

未使用过的 Norgen 拭子保存剂试管在室温下储存时，其性能可稳定到保质期，不会降低性能。请参阅设备说明书或试管标签上的保质期。

Swab Collection and DNA Preservation System Flyer

Alternate formats available upon request by emailing info@norgenbiotek.com. Such requests shall be made:

in a timely manner that considers the person's accessibility needs due to disability; and
at a cost that is no more than the regular cost charged to other persons

拭子采集和 DNA 保存系统

概述

细节

Supporting Data

Figure 1. Detection of GAPDH Using Taqman Real-time PCR from the Saliva Samples Stored at Room Temperature from 1 Week Up to 75 Months.

Figure 2. Real Time PCR Amplification of Saliva DNA from the saliva samples stored at room temperature from 1 week up to 75 months (13 time points).

Figure 3. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Swab DNA Preservative.

Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for up to 75 Months.

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Documentation

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拭子采集和 DNA 保存系统

概述

细节

Supporting Data

Figure 1. Detection of GAPDH Using Taqman Real-time PCR from the Saliva Samples Stored at Room Temperature from 1 Week Up to 75 Months.

Figure 2. Real Time PCR Amplification of Saliva DNA from the saliva samples stored at room temperature from 1 week up to 75 months (13 time points).

Figure 3. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Swab DNA Preservative.

Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for up to 75 Months.

支持数据

Documentation