Para la recogida y conservación rápida y sencilla de muestras de orina
Detalles
Supporting Data
Figure 1. Stability of DNA in Norgen's Urine Stabilizer.
Urine samples were collected from numerous healthy donors and pooled, and then the urine was aliquoted into 5 mL aliquots in Norgen's Urine Collection and Stabilization Tubes (5 cc) (Cat# 18116). The stabilized urine was stored at -20°C, 4°C and room temperature for up to 48 months. Urine DNA was subsequently isolated at time 0, 1 month, 2 months, 4 months, 8 months, 12 months, 16 months, 20 months and 24 months from the stabilized urine using Norgen's Urine DNA Isolation Kit (Slurry Format) (Cat# 48800). To test the integrity of the purified DNA, 3 μL of each elution was used in a 20 μL Taqman PCR reaction run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using Norgens 2X PCR Master Mix with 5s rRNA primers. As it can be seen, all the DNA samples were successfully amplified.
Figure 2. Stability of RNA in Norgen's Urine Stabilizer.
Urine samples were collected from numerous healthy donors and pooled, and then the urine was aliquoted into 5 mL aliquots in Norgen's Urine Collection and Stabilization Tubes (5 cc) (Cat. 18116). The stabilized urine was stored at -20°C, 4°C, and room temperature for up to 24 months. Urine RNA was then subsequently isolated at time 0, 1 month, 2 months, 4 months, 8 months, 12 months, 16 months, and 24 months from the preserved urine using Norgen's Urine Total RNA Purification Maxi Kit (Slurry Format) (Cat. 29600).
Orina seleccionada analizada con los tubos Norgen de recogida y conservación de orina
Ratón
Lince
Lobo
Orina recogida de la nieve
Tamaños y formatos disponibles
- Tubos de recogida y conservación de orina (50 cc) - Aportes de orina de 1 à 5 mL
Condiciones de almacenamiento y estabilidad del producto
Todos los tubos deben conservarse herméticamente cerrados y almacenados a temperatura ambiente (15 - 25°C) hasta 1 año sin que disminuya su rendimiento.