Sistema todo en uno para el aislamiento de ARN exosomal a partir de exosomas previamente purificados mediante los kits de purificación de exosomas de Norgen
Detalles
Supporting Data
Figure 1. Isolation of RNA from exosomes purified from different urine volumes.
Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different urine volumes purified using Norgen's Urine Exosome Purification Kits (Cat# 57700, 57800 and 57900). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The average Ct value for urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%.
Figure 2. Isolation of RNA from exosomes purified from different plasma volumes.
Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different plasma volumes purified using Norgen's Plasma/Serum Exosome Purification Kits (Cat# 57400, 57500 and 57600). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The average Ct value for plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.
Datos de respaldo
|
Especificaciones del kit
|
|
|
Tipo de muestra
|
Exosomas purificados con los kits de purificación de Norgen
|
| Tamaño del ARN purificado |
Todos los tamaños, incluidos miARN y ARN pequeños (< 200 nt)
|
| Volumen de elución |
50 à 100 μL
|
| Tiempo para completar 10 purificaciones |
35 à 40 minutos
|
| Rendimiento medio* |
Variable en función de la muestra
|
*Por favor, consulte la página 5 del prospecto del producto para conocer los rendimientos medios y los métodos comunes de cuantificación del ARN.
Condiciones de almacenamiento y estabilidad del producto
Todos los tampones deben mantenerse herméticamente cerrados y almacenados a temperatura ambiente. Este kit es estable durante 2 años a partir de la fecha de envío. Se recomienda calentar el tampón de lisis A durante 20 minutos a 60°C si se observa precipitación de sales.