Purificación rápida de ARN total -incluido microARN - a partir de pequeños volúmenes de entrada
Detalles
Supporting Data
Figure 1. Better Efficiency of RNA Recovery at Single Cell Input.
Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor’s kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit and a competitor’s spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript in the isolated RNA. RNA isolated by Norgen’s Single Cell RNA Purification Kit showed much higher relative expression of each RNA transcript tested at all cell input numbers down to single cell.
Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM).
Norgen’s Single Cell RNA Purification Kit allows sensitive but high-quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen’s Single Cell RNA Purification Kit recovered good amounts of RNA with an excellent 260/280 ratio.
Figure 3. Consistent and Linear Recovery of All Sizes of RNA down to Single Cell Input.
Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell with great linearity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit. The extracted RNA was subjected to RT-qPCR to detect all sizes of RNA, including the human S15 (mRNA), 5S rRNA transcript (small RNA), and miR-21 (microRNA). RNA isolated by Norgen’s Single Cell RNA Purification Kit showed very high consistency (over 99%) of recovery of each RNA transcript tested at all cell input numbers down to a single cell.
Datos de respaldo
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Especificaciones del kit
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Capacidad máxima de unión de la columna
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10 μg
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Volumen máximo de carga de la columna
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650 μL
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Volumen mínimo de elución
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8 μL |
| Tamaño del ARN purificado |
Todos los tamaños, incluido el ARN pequeño (<200 nt)
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Cantidad máxima de material de partida: Células animales Microdisección por captura láser (LCM) |
de una a 2 x 105 células Hasta 2 x 105 células |
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Tiempo para completar 10 purificaciones
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20 minutos
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Rendimiento medio: Células HeLa (1 x 105 células) Células HeLa (100 células) Células HeLa (1 células) |
~ 1.5 µg ~ 1.5 ng ≥ 1pg |
Condiciones de almacenamiento y estabilidad del product
Todas las soluciones deben mantenerse herméticamente cerradas y conservarse a temperatura ambiente. Este kit es estable durante 2 años a partir de la fecha de envío.