Purificación rápida de ARN total -incluido microARN - a partir de pequeñas cantidades de entrada
Detalles
Supporting Data
Figure 1. Great Isolation Sensitivity.
Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of HeLa cells. Five µL of the eluted RNA was used as the template in a 20 µL RT-qPCR reaction to detect the human S14 transcript. The S14 was detected from as little as a single cell.
Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM).
Norgen's Total RNA Purification Micro Kit allows sensitive but high quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen's Total RNA Purification Micro Kit recovered good amounts of RNA with excellent 260/280 ratio.
Figure 3. Better Efficiency of RNA Recovery at Single Cell Input.
Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor's kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen's Total RNA Purification Micro Kit and a competitor’s spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript transcript in the isolated RNA. RNA isolated by Norgen's Total RNA Purification Micro Kit showed much higher relative expression of each RNA transcript tested at all cell input number down to single cell.
Datos de respaldo
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Especificaciones del kit
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Capacidad máxima de unión a la columna
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35 μg
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Volumen máximo de carga de la columna
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650 μL
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| Volumen mínimo de elución | 20 μL |
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Tamaño del ARN purificado
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Todos los tamaños, incluido el ARN pequeño (< 200 nt) |
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Cantidad máxima de material de partida: |
5 x 105 células Hasta 7.5 mL |
| Tiempo para completar 10 purificaciones |
22 minutos
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| Rendimiento medio* Células HeLa (1 x 105 células) |
1.5 μg
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*para el tejido fibroso, se requiere un tratamiento adicional con Proteinasa K
Condiciones de almacenamiento y estabilidad del producto
Todas las soluciones deben mantenerse herméticamente cerradas y conservarse a temperatura ambiente. Este kit es estable durante 1 año a partir de la fecha de envío.