|AAV Purification Midi Kit||63300||4-8 preps|
AAV Purification Midi Kit
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV purification kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL).
|Column Binding Capacity||At least 1 x 1010 AAV particles as determined by qPCR|
|AAV Vector Serotype||Any|
|Average Recovery||> 80%|
|Input Type||Cells, media, or mixed|
8 mL - 45 mL
|Minimum Elution Volume||
|Time to Complete 4 Purifications||
2 - 2.5 hours
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. All the reagents should remain stable for at least 1 year in their unopened containers.
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