Protein Purification Kits

Inclusion Body Protein Purification

  • Solubilize and purify inclusion bodies from bacterial cell cultures using Norgen’s unique SiC spin column technology
  • Solubilize and purify inclusion bodies from bacterial cell cultures using Norgen’s unique SiC spin column technology
  • Rapid purification, independent of affinity tag utilized
  • No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
  • Versatile sample input volume
  • Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2DE, WB, MS analysis, and in vitro applications
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Protein clean-up, Concentration and Endotoxin Removal

  • Simultaneously clean-up and concentrate total isolated proteins using Norgen’s proprietary resin matrix
  • Rapidly remove salts, detergents, and endotoxins, without buffer exchange
  • Versatile sample input volume
  • No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
  • >95% protein recovery
  • Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
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Urine Protein Concentration

  • Simultaneously clean-up and concentrate total urinary proteins using Norgen’s proprietary resin matrix
  • Rapidly remove salts and urea without buffer exchange
  • Versatile sample input volume
  • No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
  • Available in a 96-well plate format for high throughput applications
  • Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
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Depletion of Abundant Serum Proteins

  • Deplete five highly abundant proteins from serum and plasma by 50-90% using Norgen’s proprietary resin matrix
  • Improve detection of low-abundance proteins for biomarker discovery
  • No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
  • Rapid, convenient, and cost-effective
  • Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
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On-Column Proteolytic Digestion

  • Simultaneously trypsinize proteins and concentrate peptides using Norgen’s proprietary resin matrix
  • Rapid, convenient, and efficient/complete digestion
  • Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
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