PCR Reagents
PCR Reagents
Reverse transcription and cDNA Synthesis
- TruScript Reverse Transcriptase is a robust enzyme with a broad range of working temperatures from 37°C to 60°C.
- TruScript Reverse Transcriptase is capable of ampiflying difficult templates with a high degree of reproducibility
- Three convenient formats available for Norgen’s TruScript Reverse Transcriptase
- TruScript Reverse Transcriptase Kit can be used for reverse-transcription reactions with any user-supplied primers
- TruScript First Strand cDNA Synthesis Kit for reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts)
- TruScript First Strand cDNA Synthesis Kit for mRNA is for reverse transcription of messenger RNA (poly A-containing transcripts)
- Convenient and cost-effective
- High sensitivity and yield
PCR Mastermix
- Ready-to-Use TaqMan Master Mixes with pre-mixed internal PCR control (HEX/VIC)
- Both Taqman PCR Master Mix and RT-PCR Master Mixes available
- Use with Norgen's TaqMan Probe/Primer and Control Sets
- For any PCR application where the input template quality is important to avoid false negatives
- Convenience and time savings
- Cost efficient
- High sensitivity
microRNA Spike-in
- Well-accepted microRNA sequence used for normalization in gene expression studies
- Best suited for RNA purification from samples with low RNA abundance including liquid biopsies (plasma/serum/urine etc.) and low cell or tissue inputs
- Compatible with expression analysis using RT-qPCR - both RNA and matching forward PCR primer provided
- Fully compatible with Norgen's microScript cDNA Synthesis system
- Fully compatible with Next Generation Sequencing (Small RNA-Seq) library preparation workflow
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