PCR Reagents

Reverse transcription and cDNA Synthesis

TruScript Reverse Transcriptase is a robust enzyme with a broad range of working temperatures from 37°C to 60°C.
TruScript Reverse Transcriptase is capable of amplifying difficult templates with a high degree of reproducibility
Three convenient formats available for Norgen’s TruScript Reverse Transcriptase
- TruScript Reverse Transcriptase Kit can be used for reverse-transcription reactions with any user-supplied primers
- TruScript First Strand cDNA Synthesis Kit for reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts)
- TruScript First Strand cDNA Synthesis Kit for mRNA is for reverse transcription of messenger RNA (poly A-containing transcripts)
Convenient and cost-effective
High sensitivity and yield

Ready-to-Use TaqMan Master Mixes with pre-mixed internal PCR control (HEX/VIC)
Both Taqman PCR Master Mix and RT-PCR Master Mixes available
Use with Norgen's TaqMan Probe/Primer and Control Sets
For any PCR application where the input template quality is important to avoid false negatives
Convenience and time savings
Cost efficient
High sensitivity

Well-accepted microRNA sequence used for normalization in gene expression studies
Best suited for RNA purification from samples with low RNA abundance including liquid biopsies (plasma/serum/urine etc.) and low cell or tissue inputs
Compatible with expression analysis using RT-qPCR - both RNA and matching forward PCR primer provided
Fully compatible with Norgen's microScript cDNA Synthesis system
Fully compatible with Next Generation Sequencing (Small RNA-Seq) library preparation workflow