Reverse transcription and cDNA Synthesis

  • TruScript Reverse Transcriptase is a robust enzyme with a broad range of working temperatures from 37°C to 60°C.
  • TruScript Reverse Transcriptase is capable of amplifying difficult templates with a high degree of reproducibility
  • Three convenient formats available for Norgen’s TruScript Reverse Transcriptase
    • TruScript Reverse Transcriptase Kit can be used for reverse-transcription reactions with any user-supplied primers
    • TruScript First Strand cDNA Synthesis Kit for reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts)
    • TruScript First Strand cDNA Synthesis Kit for mRNA is for reverse transcription of messenger RNA (poly A-containing transcripts)
  • Convenient and cost-effective
  • High sensitivity and yield

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PCR Mastermix

  • Ready-to-Use TaqMan Master Mixes with pre-mixed internal PCR control (HEX/VIC)
  • Both Taqman PCR Master Mix and RT-PCR Master Mixes available
  • Use with Norgen's TaqMan Probe/Primer and Control Sets
  • For any PCR application where the input template quality is important to avoid false negatives
  • Convenience and time savings
  • Cost efficient
  • High sensitivity

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microRNA Spike-in

  • Well-accepted microRNA sequence used for normalization in gene expression studies
  • Best suited for RNA purification from samples with low RNA abundance including liquid biopsies (plasma/serum/urine etc.) and low cell or tissue inputs
  • Compatible with expression analysis using RT-qPCR - both RNA and matching forward PCR primer provided
  • Fully compatible with Norgen's microScript cDNA Synthesis system
  • Fully compatible with Next Generation Sequencing (Small RNA-Seq) library preparation workflow

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