Next Generation Sequencing Services

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16S rRNA Microbiome Sequencing

  • Comprehensive Service
  • Various Sample Types including Environmental
  • Illumina® MiSeq Sequencing Platform
  • Analyze Entire Bacterial Community
  • Identify Phylogenetic or Taxonomic Classifications
  • Fast Turn Around Time

Norgen Biotek offers comprehensive services for Next-Generation Sequencing (NGS) in an accredited state-of-the-art laboratory from sample isolation to sequencing and bioinformatics analysis. We have extensive expertise in sample preparation, sequencing and analysis of all types of environmental and human samples including soil, water, agricultural products, stool, saliva, blood and more.


saliva preservative

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Our Comprehensive Workflow


Step 1: Consultation

It is essential to have an initial engagement regarding your sequencing project with our experts to define and set clear objectives. We will discuss with you various elements of your project to learn how we can best achieve your goals. Our NGS scientists and bioinformaticians will be happy to learn about your project and make the best recommendations to suit your project needs.

Please contact us to schedule your complimentary consultation.

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Step 2a: Sample Submission

We will provide detailed instructions on how to handle, process and ship your samples to our facility. We accept both purified DNA and specimens for DNA isolation services. In addition, we offer a complete guideline on sample collection and preservation. Recommended specimen input and handling precautions for DNA purification can be found in our 16s rRNA-Seq Service Guide.

How will you submit your samples?

  1. I still need to collect my samples
  2. I will send specimens
  3. I will send purified DNA
Sample Submission

Step 2b: Shipping

For detailed shipping instructions, please refer to the document below.

Shipping Instructions
Shipping

Step 3: Sample Isolation

Our 16s rRNA Sequencing service includes DNA isolation. We have tremendous experience and expertise in isolating DNA from various sample types. Our service representatives will guide you on the amount of sample we require for any isolation.

Total RNA Purification Kit

Step 4: DNA Quality Control

We perform quality control on the isolated DNA to assess quantity, quality, and amplifiability prior to library prep. We can share this data prior to proceeding with sequencing at your request.

Total RNA Purification Kit

Step 5: Library Preparation

We generate DNA libraries using Norgen Biotek’s Metagenomics Library Prep Kits (for Illumina) based on the target region of your choice. The DNA library will then undergo a QC step prior to sequencing.

Small RNA Library Prep Kit

Step 6: Sequencing

We will sequence the sample libraries on the Illumina platform to achieve your required coverage and read depth.


Total RNA Purification Kit

Step 7: Analysis

We have full bioinformatics capabilities from basic analysis to advanced analysis pipelines to help make sense of your data. All our 16s rRNA services include basic bioinformatics analysis as a deliverable in your final report.

Irenaeus NETWORK

Final Report


Let's get your project started.

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