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Norgen Biotek offers comprehensive services for Next-Generation Sequencing (NGS) in an accredited state-of-the-art laboratory from sample isolation to sequencing and bioinformatics analysis. We have extensive expertise in sample preparation, sequencing and analysis of all types of environmental and human samples including soil, water, agricultural products, stool, saliva, blood and more.
Figure 1. Illumina MiSeq 16s rRNA microbiome data from saliva samples preserved for over 6 years using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). The saliva DNA was isolated using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram
Figure 1. Illumina MiSeq 16s rRNA microbiome data from saliva samples preserved for over 6 years using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). The saliva DNA was isolated using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram
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Figure 2. High quality soil DNA was successfully isolated from 5 challenging soil types using (A) Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) and (B) Norgen’s Soil DNA Isolation respectively. The purified DNA was then successfully used in 16S rRNA Microbiome Sequencing. The Hierarchical Clustering Dendrogram shown is based on genus-level classifications and shows the relative abundance of its genus-level classifications among soil types.
Figure 2. High quality soil DNA was successfully isolated from 5 challenging soil types using (A) Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) and (B) Norgen’s Soil DNA Isolation respectively. The purified DNA was then successfully used in 16S rRNA Microbiome Sequencing. The Hierarchical Clustering Dendrogram shown is based on genus-level classifications and shows the relative abundance of its genus-level classifications among soil types.
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Figure 3. Principal Coordinate Analysis (PCoA) generated by Illumina MiSeq. Principal Coordinate Analysis of 20 samples (25-28: Mulch, 29-32: Clay, 33-36: Potting soil, 76-78: Top soil, 79-82: Compost) showing differences in the distribution of taxonomic classifications between samples up to species level. Each soil type is clearly clustered together and away from other types
Figure 3. Principal Coordinate Analysis (PCoA) generated by Illumina MiSeq. Principal Coordinate Analysis of 20 samples (25-28: Mulch, 29-32: Clay, 33-36: Potting soil, 76-78: Top soil, 79-82: Compost) showing differences in the distribution of taxonomic classifications between samples up to species level. Each soil type is clearly clustered together and away from other types
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Figure 4.Pane A: Principal Coordinate Analysis of 10 fecal microbiomes showing differences in the distribution of taxonomic classifications between samples up to class level. Panel B: Hierarchical clustering of 10 fecal microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.
Figure 4.Pane A: Principal Coordinate Analysis of 10 fecal microbiomes showing differences in the distribution of taxonomic classifications between samples up to class level. Panel B: Hierarchical clustering of 10 fecal microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.
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Figure 5.16S microbiome data generated by Illumina MiSeq. Stool DNA was isolated using Norgen’s Stool DNA Isolation Kit (Magnetic Bead System) from 200 mg of stool from healthy donors, and the stool microbiomes were successfully sequenced by Illumina MiSeq.
Figure 5.16S microbiome data generated by Illumina MiSeq. Stool DNA was isolated using Norgen’s Stool DNA Isolation Kit (Magnetic Bead System) from 200 mg of stool from healthy donors, and the stool microbiomes were successfully sequenced by Illumina MiSeq.
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1. Consultation 2. Sample Submission/Shipping 3. Sample Isolation 4. DNA Quality Control
5. Library Preparation 6. Sequencing 7. Analysis 8. Final Report
It is essential to have an initial engagement regarding your sequencing project with our experts to define and set clear objectives. We will discuss with you various elements of your project to learn how we can best achieve your goals. Our NGS scientists and bioinformaticians will be happy to learn about your project and make the best recommendations to suit your project needs.
Please contact us to schedule your complimentary consultation.
We will provide detailed instructions on how to handle, process and ship your samples to our facility. We accept both purified DNA and specimens for DNA isolation services. In addition, we offer a complete guideline on sample collection and preservation. Recommended specimen input and handling precautions for DNA purification can be found in our 16s rRNA-Seq Service Guide.
How will you submit your samples?
For detailed shipping instructions, please refer to the document below.
Our 16s rRNA Sequencing service includes DNA isolation. We have tremendous experience and expertise in isolating DNA from various sample types. Our service representatives will guide you on the amount of sample we require for any isolation.
We perform quality control on the isolated DNA to assess quantity, quality, and amplifiability prior to library prep. We can share this data prior to proceeding with sequencing at your request.
We generate DNA libraries using Norgen Biotek’s Metagenomics Library Prep Kits (for Illumina) based on the target region of your choice. The DNA library will then undergo a QC step prior to sequencing.
We will sequence the sample libraries on the Illumina platform to achieve your required coverage and read depth.
We have full bioinformatics capabilities from basic analysis to advanced analysis pipelines to help make sense of your data. All our 16s rRNA services include basic bioinformatics analysis as a deliverable in your final report.
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