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Norgen Biotek offers comprehensive services for Next-Generation Sequencing (NGS) in an accredited state-of-the-art laboratory from sample isolation to sequencing and bioinformatics analysis. We have extensive expertise in sample preparation, sequencing and analysis of all types of environmental and human samples including soil, water, agricultural products, stool, saliva, blood and more.
Figure 1. The Alpha rarefaction curve is used to assess species richness. The curve is generated by by calculating the amount of different OTUs (operational taxonomic unit) in relation to sample size.
Figure 1.The Alpha rarefaction curve is used to assess species richness. The curve is generated by by calculating the amount of different OTUs (operational taxonomic unit) in relation to sample size.
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Figure 2. Illumina MiSeq 16S rRNA data from saliva samples preserved for over 6 years using Norgen’s Saliva DNA Collection and Preservation Device (Cat. RU49000). The saliva DNA was isolated using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. The relative abundance (%) is shown per each sample, and shows the top 10 most abundant by Genus.
Figure 2. Illumina MiSeq 16S rRNA data from saliva samples preserved for over 6 years using Norgen’s Saliva DNA Collection and Preservation Device (Cat. RU49000). The saliva DNA was isolated using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. The relative abundance (%) is shown per each sample, and shows the top 10 most abundant by Genus.
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Figure 3. 16S microbiome data generated by Illumina MiSeq. Stool DNA was isolated using Norgen’s Stool Isolation Kit (Magenetic Bead System)(Cat. 55700) from 200 mg of stool from 9 donors. The Shannons Diversity is a common Alpha Diversity metric which measures the richness and diversity of a population.
Figure 3. 16S microbiome data generated by Illumina MiSeq. Stool DNA was isolated using Norgen’s Stool Isolation Kit (Magenetic Bead System)(Cat. 55700) from 200 mg of stool from 9 donors. The Shannons Diversity is a common Alpha Diversity metric which measures the richness and diversity of a population.
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Figure 4.High quality soil DNA was successfully isolated from soil samples using (A) Norgen’s Soil DNA Isolation Kit (Magnetic Bead System)(Cat. 58100) and (B) Norgen’s Soil DNA Isolation Plus Kit (Cat. 64000) respectively. The purified DNA was then successfully used in 16S rRNA Microbiome Sequencing. Once sequenced samples are assembled into OTUs (operational taxonomic unit) based on 97% identity and the Phylogenetic tree represents the evolutionary relationship between these OTUs.
Figure 4.High quality soil DNA was successfully isolated from soil samples using (A) Norgen’s Soil DNA Isolation Kit (Magnetic Bead System)(Cat. 58100) and (B) Norgen’s Soil DNA Isolation Plus Kit (Cat. 64000) respectively. The purified DNA was then successfully used in 16S rRNA Microbiome Sequencing. Once sequenced samples are assembled into OTUs (operational taxonomic unit) based on 97% identity and the Phylogenetic tree represents the evolutionary relationship between these OTUs.
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Figure 5.Principal Coordinate Analysis (PCoA) of 26 samples which shows the differences in the distribution in of taxonomic classification between samples from a genus level, using an Unweighted Unifrac metric. The Unweighted Unifrac metric is sensitive to differences in low-abundant features.
Figure 5.Principal Coordinate Analysis (PCoA) of 26 samples which shows the differences in the distribution in of taxonomic classification between samples from a genus level, using an Unweighted Unifrac metric. The Unweighted Unifrac metric is sensitive to differences in low-abundant features.
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1. Consultation 2. Sample Submission/Shipping 3. Sample Isolation 4. DNA Quality Control
5. Library Preparation 6. Sequencing 7. Analysis 8. Final Report
It is essential to have an initial engagement regarding your sequencing project with our experts to define and set clear objectives. We will discuss with you various elements of your project to learn how we can best achieve your goals. Our NGS scientists and bioinformaticians will be happy to learn about your project and make the best recommendations to suit your project needs.
Please contact us to schedule your complimentary consultation.
We will provide detailed instructions on how to handle, process and ship your samples to our facility. We accept both purified DNA and specimens for DNA isolation services. In addition, we offer a complete guideline on sample collection and preservation. Recommended specimen input and handling precautions for DNA purification can be found in our 16S and ITS rRNA-Seq Service Guide.
How will you submit your samples?
For detailed shipping instructions, please refer to the document below.
Our 16S and ITS rRNA Sequencing service includes DNA isolation. We have tremendous experience and expertise in isolating DNA from various sample types. Our service representatives will guide you on the amount of sample we require for any isolation.
We perform quality control on the isolated DNA to assess quantity, quality, and amplifiability prior to library prep. We can share this data prior to proceeding with sequencing at your request.
We generate DNA libraries using Norgen Biotek’s Metagenomics Library Prep Kits (for Illumina) based on the target region of your choice. The DNA library will then undergo a QC step prior to sequencing.
We will sequence the sample libraries on the Illumina platform to achieve your required coverage and read depth.
We have full bioinformatics capabilities from basic analysis to advanced analysis pipelines to help make sense of your data. All our 16S and ITS rRNA services include basic bioinformatics analysis as a deliverable in your final report
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