1. Consultation
Request a free consultation with our metagenomics experts to discuss your research goals and receive tailored recommendations.
Request a Consultation
Sequencing Services
Metagenomics Sequencing
Key Features
In our accredited state-of-the-art laboratory, we offer comprehensive Next-Generation Sequencing (NGS) services to provide full workflow solutions, from DNA isolation and sequencing to advanced bioinformatic analyses for ready-to-publish data. Our experienced staff have extensive expertise working with a variety of environmental and human sample types, including stool, saliva, soil, water and more. We are here to help!
Can work with challenging sample types (soil, plant, etc.)
Illumina® MiSeq Sequencing Platform
Analyze entire bacterial communities with comprehensive bioinformatics analysis
Fast Turn Around Time
3-4 weeks
Our NGS Clients
We are pleased to work with some of the most prestigious companies and institutions in 155+ countries.
Your Custom Workflow
2. Sample Submission
For sample preparation guidelines, check out these links.
For detailed shipping instructions, please refer to the document below.
Shipping Instructions
3. Sample Isolation
With tremendous experience and expertise in isolating DNA from various sample types, our NGS team can work with even the most challenging samples. Isolation is performed using Norgen Biotek's proprietary silicon carbide technology.
4. DNA Quality Control
You can be confident that the sequencing portion of your project will be successful as we perform quality control on the isolated DNA to assess quantity, quality, and amplifiability prior to library preparation.
5. Library Preparation
Norgen offers 16S Metagenomics Sequencing for any of the 9 target regions of the 16S gene. Based on the target region. Norgen Biotek provides library preparation kits for 16S rRNA and ITS sequencing. The DNA library will then undergo a quality check prior to sequencing.
7. Bioinformatics Analysis
Our basic analysis includes taxonomic classification down to the phylum and genus level, Shannon diversity index, OTU (operational taxonomic unit) classification with 97% similarity identity, weighted and unweighted diversity and rooted phylogenetic relationships.
8. Final Report
We deliver a detailed report of methods and results in a clear, publication-ready format.
Sample Report16S Variable Region Interactive Tool
Using the Interactive Variable Region Diagram, select any of the regions below to learn about which variable region may be best suited for your sequencing project.
Region V1 to V2 (Nucleotides 69-242)
- Used to distinguish Streptococcus sp. and differentiate between Staphylococcus aureus and coagulase negative Staphylococcus species
- Able to distinguish among Staphylococcal, Streptococcal, Clostridium, Haemophilus and Neisseria species
- Best target for distinguishing among Mycobacterium sp.
- Able to detect Actinomycetaceae, Bacillaceae, Bacteriodaceae, Clostridiaceae, Deinococcaceae, Enterococcaceae, Helicobacteraceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Propionibacteriaceae, Pseudomonadaceae, Rhodobacteraceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at the Family level only
- Not useful for most Escherichia sp., Shigella sp., K. pneumoniae and E. aerogene
Region V1 to V3 (Nucleotides 69-497)
- Used to distinguish Staphylococci populations
- Able to detect Prevotella, Porphyromonas, and Bacteroides
Region V1 to V2 (Nucleotides 69-242)
- Used to distinguish Streptococcus sp. and differentiate between Staphylococcus aureus and coagulase negative Staphylococcus species
- Able to distinguish among Staphylococcal, Streptococcal, Clostridium, Haemophilus and Neisseria species
- Best target for distinguishing among Mycobacterium sp.
- Able to detect Actinomycetaceae, Bacillaceae, Bacteriodaceae, Clostridiaceae, Deinococcaceae, Enterococcaceae, Helicobacteraceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Propionibacteriaceae, Pseudomonadaceae, Rhodobacteraceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at the Family level only
- Not useful for most Escherichia sp., Shigella sp., K. pneumoniae and E. aerogene
Region V1 to V3 (Nucleotides 69-497)
- Used to distinguish Staphylococci populations
- Able to detect Prevotella, Porphyromonas, and Bacteroides
Region V1 to V2 (Nucleotides 69-242)
- Used to distinguish Streptococcus sp. and differentiate between Staphylococcus aureus and coagulase negative Staphylococcus species
- Able to distinguish among Staphylococcal, Streptococcal, Clostridium, Haemophilus and Neisseria species
- Best target for distinguishing among Mycobacterium sp.
- Able to detect Actinomycetaceae, Bacillaceae, Bacteriodaceae, Clostridiaceae, Deinococcaceae, Enterococcaceae, Helicobacteraceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Propionibacteriaceae, Pseudomonadaceae, Rhodobacteraceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at the Family level only
- Not useful for most Escherichia sp., Shigella sp., K. pneumoniae and E. aerogene
Region V2 to V3 (Nucleotides 137-497)
- Targets speciation among Staphylococcal and Streptococcal pathogens as well as Clostridium and Niesseria, Mycobacterium, and Haemophilius
Region V1 to V3 (Nucleotides 69-497)
- Used to distinguish Staphylococci populations
- Able to detect Prevotella, Porphyromonas, and Bacteroides
Region V2 to V3 (Nucleotides 137-497)
- Targets speciation among Staphylococcal and Streptococcal pathogens as well as Clostridium and Niesseria, Mycobacterium, and Haemophilius
Region V1 to V3 (Nucleotides 69-497)
- Used to distinguish Staphylococci populations
- Able to detect Prevotella, Porphyromonas, and Bacteroides
Region V2 to V3 (Nucleotides 137-497)
- Targets speciation among Staphylococcal and Streptococcal pathogens as well as Clostridium and Niesseria, Mycobacterium, and Haemophilius
Region V3 to V4 (Nucleotides 433-682)
- Better than region V2 in distinguishing between enterobacteriaceae K. pneumoniae and E. aerogenes
- Able to detect Actinomycetaceae, Bacillaceae, Bacteroidaceae, Clostridiaceae, Enterococcaceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Pseudomonadaceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at the Family level only
Region V1 to V3 (Nucleotides 69-497)
- Used to distinguish Staphylococci populations
- Able to detect Prevotella, Porphyromonas, and Bacteroides
Region V3 to V5 (Nucleotides 433-879)
- Able to detect Coxiellaceae, Enterococcaceae, and unclassified families within Firmicutes amd Tenericutes
Region V3 to V4 (Nucleotides 433-682)
- Better than region V2 in distinguishing between enterobacteriaceae K. pneumoniae and E. aerogenes
- Able to detect Actinomycetaceae, Bacillaceae, Bacteroidaceae, Clostridiaceae, Enterococcaceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Pseudomonadaceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at the Family level only
Region V3 to V5 (Nucleotides 433-879)
- Able to detect Coxiellaceae, Enterococcaceae, and unclassified families within Firmicutes amd Tenericutes
Region V4 (Nucleotides 576-682)
- Able to detect Actinomycetaceae, Bacillaceae, Bacteriodaceae, Clostridiaceae, Deinococcaceae, Enterococcaceae, Helicobacteraceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Propionibacteriaceae, Rhodobacteraceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae, Neisseriaceae, Pseudomonadaceae at the Family level only
Region V3 to V4 (Nucleotides 433-682)
- Better than region V2 in distinguishing between enterobacteriaceae K. pneumoniae and E. aerogenes
- Able to detect Actinomycetaceae, Bacillaceae, Bacteroidaceae, Clostridiaceae, Enterococcaceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Pseudomonadaceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at the Family level only
Region V4 to V5 (Nucleotides 576-879)
- Able to detect P. acnes and B. cepacia
Region V3 to V5 (Nucleotides 433-879)
- Able to detect Coxiellaceae, Enterococcaceae, and unclassified families within Firmicutes amd Tenericutes
Region V4 to V5 (Nucleotides 576-879)
- Able to detect P. acnes and B. cepacia
Region V3 to V5 (Nucleotides 433-879)
- Able to detect Coxiellaceae, Enterococcaceae, and unclassified families within Firmicutes amd Tenericutes
Region V4 to V5 (Nucleotides 576-879)
- Able to detect P. acnes and B. cepacia
Region V3 to V5 (Nucleotides 433-879)
- Able to detect Coxiellaceae, Enterococcaceae, and unclassified families within Firmicutes amd Tenericutes
Region V5 to V7 (Nucleotides 822-1173)
- Able to detect Cyanobacteria and picocyanobacterial communities, such as Synechococcus, Prochlorococcus, and Cyanobium
Region V5 to V7 (Nucleotides 822-1173)
- Able to detect Cyanobacteria and picocyanobacterial communities, such as Synechococcus, Prochlorococcus, and Cyanobium
Region V5 to V7 (Nucleotides 822-1173)
- Able to detect Cyanobacteria and picocyanobacterial communities, such as Synechococcus, Prochlorococcus, and Cyanobium
Region V5 to V7 (Nucleotides 822-1173)
- Able to detect Cyanobacteria and picocyanobacterial communities, such as Synechococcus, Prochlorococcus, and Cyanobium
Region V5 to V7 (Nucleotides 822-1173)
- Able to detect Cyanobacteria and picocyanobacterial communities, such as Synechococcus, Prochlorococcus, and Cyanobium
Region V7 to V9 (Nucleotides 1117-1465)
- Able to detect Veillonella populations
- Able to detect Deinococcaceae, Moraxellaceae, and Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at Family level only
Region V7 to V9 (Nucleotides 1117-1465)
- Able to detect Veillonella populations
- Able to detect Deinococcaceae, Moraxellaceae, and Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at Family level only
Region V7 to V9 (Nucleotides 1117-1465)
- Able to detect Veillonella populations
- Able to detect Deinococcaceae, Moraxellaceae, and Streptococcaceae at both the Family and Genus level
- Able to detect Enterobacteriaceae at Family level only
Sample Collection and Preservation
Filter:
SKU 76200 | 1 System
USD$14.00
Enables simple, non-invasive at-home urine collection and preserves nucleic acids at ambient temperature.
Use code RAPIDUSER at checkout to receive 50% off.
Limited Stock. Discount applies only to products that are at 50% of their shelf life.
SKU 69900 | 1 Device
USD$17.00
Norgen's EZCollect (Easy Collect) Saliva DNA Device Dx provides a safe and rapid method for home-based collection, and preservation of saliva DNA.
SKU 69800 | 1 Device
USD$17.00
For safe and rapid home-based self collection, and preservation of saliva RNA.
SKU RU69800 | 1 Device
USD$16.00
Norgen's EZCollect (Easy Collect) Saliva RNA Device provides a safe and rapid method for home-based collection, and preservation of saliva RNA.
SKU RU69900 | 1 Device
USD$16.00
Norgen's EZCollect (Easy Collect) Saliva DNA Device provides a safe and rapid method for home-based collection, and preservation of saliva DNA.
Sample Available
SKU 63700 | 50 Devices
USD$1,107.00
All-in-one system for the rapid and simple collection, preservation, and room temperature storage and shipping of stool samples.
Sample Available
SKU 45670-B | 50 Units
USD$359.00
A rapid and simple method for the collection, preservation and shipping of stool samples
Sample Available
SKU 45690-B | 50 Units
USD$355.00
For collection, ambient storage and transport of DNA from samples collected using a swab
SKU 53800 | 50 Devices
USD$648.00
Preserve High Quality RNA, including Viral RNA, for up to 2 Months
SKU Dx69200 | 50 Tubes
USD$359.00
Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab
Sample Available
SKU 69200 | 50 Tubes
USD$345.00
For ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab
SKU 18129 | 120 cc Cup | 1 Cup
USD$15.00
For rapid and simple collection, and preservation of urine samples
Library Preparation Kits
Filter:
SKU 70740 | 96 Preps Set D
USD$1,019.00
The reagents and components required for library preparation of the 16S V4-V5 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70900 | 24 Preps
USD$329.00
The reagents and components required for library preparation of the 16S V7-V9 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70840 | 96 Preps Set D
USD$1,019.00
The reagents and components required for library preparation of the 16S V5-V7 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70830 | 96 Preps Set C
USD$1,019.00
The reagents and components required for library preparation of the 16S V5-V7 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70820 | 96 Preps Set B
USD$1,019.00
The reagents and components required for library preparation of the 16S V5-V7 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70810 | 96 Preps Set A
USD$1,019.00
The reagents and components required for library preparation of the 16S V5-V7 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70800 | 24 Preps
USD$329.00
The reagents and components required for library preparation of the 16S V5-V7 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70710 | 96 Preps Set A
USD$1,019.00
The reagents and components required for library preparation of the 16S V4-V5 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70630 | 96 Preps Set C
USD$1,019.00
The reagents and components required for library preparation of the 16S V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70640 | 96 Preps Set D
USD$1,019.00
The reagents and components required for library preparation of the 16S V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70700 | 24 Preps
USD$329.00
The reagents and components required for library preparation of the 16S V4-V5 amplicon libraries to be used for next-generation sequencing on Illumina platforms
SKU 70910 | 96 Preps Set A
USD$1,019.00
The reagents and components required for library preparation of the 16S V7-V9 amplicon libraries to be used for next-generation sequencing on Illumina platforms
Sequencing Platform
Illumina MiSeq
Key Technologies
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Sequencing by synthesis chemistry
The MiSeq System leverages proven Illumina sequencing by synthesis (SBS) chemistry to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel. -
Paired-end sequencing
The MiSeq System incorporates paired-end sequencing that enables both ends of the DNA fragment to be sequenced. Because the distance between each paired read is known, alignment algorithms can use this information to map the reads. This is especially helpful across difficult-to-sequence, repetitive regions of the genome.
Bioinformatics Analysis
Figure 1. Illumina MiSeq 16S rRNA data from saliva samples preserved for over 6 years using Norgen's Saliva DNA Collection and Preservation Device (Cat. RU49000). The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. The relative abundance (%) is shown per each sample, and shows the top 10 most abundant by Genus.
Frequently Asked Questions
What is Next Generation Sequencing?
Next Generation Sequencing (NGS) is an important tool used in the field of genomic research as it allows for high throughput and massively parallel sequencing of thousands of molecules simultaneously. NGS provides researchers with fast, scalable solutions for a clear, complete picture of their samples to advance their scientific discoveries.
What is bioinformatics?
Bioinformatics has quickly advanced to become an important and powerful application in biological research. It involves a combination of computational and statistical methods to better understand and analyze scientific data, guiding the research community to new opportunities and novel discoveries. As an emerging discipline, bioinformatics continues to be an essential tool for researchers in many fields including modern molecular biology and clinical research, with its applications leading to significant medical advancements and developments in future research to come.
Why should I choose Norgen for my microbiome sequencing?
Norgen Biotek is an ISO-certified state-of-the-art laboratory specializing in Next Generation Sequencing using the Illumina platform. We provide comprehensive solutions for a wide range of applications including healthcare, agriculture and environmental monitoring. Our expert scientists have vast experience with challenging sample types and we strive to ensure we support our customers at every stage of their project. We pride ourselves on providing our customers with quality data to accelerate their research in microbiome advancements.
Do you offer extraction services?
Yes. Our comprehensive 16S rRNA Sequencing service includes DNA extraction and QC from any sample type.
What are your sample submission requirements?
Our requirements for sample submission can be found under Step 2: Sample Submission on this page.
What amplicon regions do you sequence?
We offer 9 primers which target the 9 regions of the 16S rRNA gene. Ranging from V1-V9, each target has a specificity to certain bacterial profiles. For instance, the commonly used V3-V4 region is great for detecting most bacteria at a genus level. If you aren't sure which region is best for your samples, you can use our interactive Variable Region Diagram, or you can always consult with us and our NGS scientists would be happy to make a recommendation.
What sequencing platform do you sequence on?
We sequence all 16S Metagenomics Sequencing projects on the Illumina MiSeq platform.
What is the depth of coverage Norgen provides?
Norgen's standard 16S rRNA Sequencing service provides an average of 50K* raw reads per sample. * May differ for low biomass samples. Please contact services@norgenbiotek.com for our recommendations if you are working with low biomass samples.
Does the price include bioinformatics analysis?
Yes. Our comprehensive service includes a full bioinformatics analysis of your raw data. We deliver the bioinformatics analysis in a PDF report and we also provide you with a secure link to download all of your raw data and intermediate files.
What is the typical turnaround time?
Our typical turnaround time is 3-4 weeks from the time of sample receipt. The turnaround time is dependent on the size of your project. Please contact services@norgenbiotek.com to receive an accurate estimated turnaround time.
Norgen is located in Canada. Do you still accept samples from othercountries?
Absolutely! We accept samples from countries worldwide and our NGS team will be happy to assist you with making the shipping arrangements.
Citations
| Title | Effects of a 10-Strain Oral Probiotic on Parameters of Vaginal Health and Microbial Community: A Pilot Clinical Study |
| Journal | Int J Womens Health. 2022. |
| Authors | Christopher J Martoni, Amalie Kruse Sigersted Frederiksen, Anders Damholt, and Gregory Leyer |
| Title | Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis |
| Journal | Journal of Veterinary Internal Medicine. 2022. |
| Authors | Emily L. Coffey, Andres M. Gomez, Erin N. Burton, Jennifer L. Granick, Jody P. Lulich, Eva Furrow |