Norgen Biotek offers comprehensive services for Next-generation sequencing (NGS) using the Illumina Platform in an accredited state-of-the-art laboratory with extensive expertise in sample preparation, sequencing and analysis of all types of environmental and human microbiome samples including soil, water, agricultural products, stool, saliva, blood, etc.
Our expert staff will assist in the initial consultation from sample handling, depth of reads that will be required to answer your questions, the specific variable regions targeted, sample multiplexing per run, to the final analysis of data presented in an easy to understand report. Your project will receive full attention by experts and will guarantee fast turn-around time. Our services include sample isolation, DNA QC, library preparation, sequencing, and analysis of data.
Figure 1. Illumina MiSeq 16s rRNA microbiome data from saliva samples preserved for over 6 years using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). The saliva DNA was isolated using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.
Figure 2. High quality of soil DNA was successfully isolated from the 5 challenging soil types using Norgen’s Soil DNA Isolation Kits (A: Soil DNA Isolation Kit (Magnetic Bead System) and B: Soil DNA Isolation Kit Spin Column) respectively. The Hierarchical Clustering Dendrogram is based on genus-level classifications and shows the relative abundance of its genus-level classifications among soil types.
Figure 3. Principal Coordinate Analysis (PCoA) generated by Illumina MiSeq. Principal Coordinate Analysis of 20 samples (25-28: Mulch, 29-32: Clay, 33-36: Potting soil, 76-78: Top soil, 79-82: Compost) showing differences in the distribution of taxonomic classifications between samples up to species level. Each soil type is clearly clustered together and away from other types.
Figure 4. A) Principal Coordinate Analysis of 10 fecal microbiomes showing differences in the distribution of taxonomic classifications between samples up to class level. B) Hierarchical clustering of 10 fecal microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.
Figure 5. 16S microbiome data generated by Illumina MiSeq. Stool DNA was isolated using Norgen’s Stool DNA Isolation Kit (Magnetic Bead System) from 200 mg of stool from healthy donors, and the stool microbiomes was successfully sequenced by Illumina MiSeq.
|Sample Type||Kit Name||Cat. Number|
|Fecal Samples||Stool DNA Isolation Kit|
|Soil||Soil DNA Isolation Kit|
|Blood||Blood DNA Isolation Mini Kit|
|Urine||Urine DNA Isolation Kit for Exfoliated Cells or Bacteria|
|Bacteria||Bacterial Genomic DNA Isolation Kit|
|Fungi/Yeast||Fungi/Yeast Genomic DNA Isolation Kit||27300|
|Milk||Milk Bacteria DNA Isolation Kit||21550|
|Saliva||Saliva DNA Isolation Kit||RU45400|
|Water||Water RNA/DNA Purification Kit||26450, 26400|
|Plant/Fungi||Plant/Fungi DNA Isolation Kit||26200, 26900|
|Cells and Tissue||Cells and Tissue DNA Isolation Kit||53100|
|Sequencing Clean-up||Sequencing Reaction Clean-up Kit||34500, 34400|
Expert advice on sample handling, processing and experimental design for best data outcome
You can elect to simply submit isolated DNA or send the samples for DNA isolation; our team will guide you through what is required from different sample types
Isolated DNA will be quantified, assessed for quality and PCR amplification
Library preparation with industry standard kits, QC and quantification
Filtering, aligning/mapping, annotating with statistical analysis
Comprehensive report detailing the analysis will be the final deliverable
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