Metagenomics Sequencing

Metagenomics Sequencing

  • Comprehensive Service for 16S rRNA
  • DNA extraction service included
  • Easy to Challenging Sample Types including Environmental
  • Illumina® MiSeq Sequencing Platform
  • Analyze Entire Bacterial Communities with Comprehensive Bioinformatics Analysis
  • Identify Phylogenetic or Taxonomic Classifications
  • Fast Turn Around Time | 3 - 4 Weeks

In our accredited state-of-the-art laboratory, we offer comprehensive Next-Generation Sequencing (NGS) services to provide full workflow solutions, from DNA isolation and sequencing to advanced bioinformatic analyses for ready-to-publish data. Our experienced staff have extensive expertise working with a variety of environmental and human sample types, including stool, saliva, soil, water and more. We are here to help!

Next Generation Sequencing

Our NGS Clients

We are pleased to work with some of the most prestigious companies and institutions in 155+ countries.

Bioinformatics Analysis

Your Custom Workflow


Step 1: Consultation

Get started by requesting your free consultation with one of our metagenomics experts. We would love to discuss your research objectives and are happy to provide assistance in defining your project goals. Our NGS specialists and bioinformaticians are here to provide customized recommendations to suit your project needs.

Request Your Consultation

Step 2: Sample Submission & Shipping

Shipping your samples to our facility is easy and painless! We’ll provide you with detailed instructions on how to handle, process and ship your samples to our laboratory. We accept both purified DNA and specimens for DNA isolation services.

If you are shipping specimens to us, a complete guide on sample collection and preservation will be provided to you. Recommended specimen input and handling procedures for DNA purification can be found in our Metagenomics Service Guide.

How will you submit your samples?

  1. I still need to collect my samples
  2. I will send specimens
  3. I will send purified DNA

For detailed shipping instructions, please refer to the document below.

Shipping Instructions

Step 3: Sample Isolation

DNA isolation is included in your 16S rRNA. With tremendous experience and expertise in isolating DNA from various sample types, our NGS team can work with even the most challenging samples.

Step 4: DNA Quality Control

You can be confident that the sequencing portion of your project will be successful as we perform quality control on the isolated DNA to assess quantity, quality, and amplifiability prior to library preparation.

Step 5: Library Preparation

Norgen offers 16S Metagenomics Sequencing for any of the 9 target regions of the 16S gene. Based on the target region of your choice, we generate DNA libraries using Norgen Biotek’s Metagenomics Library Prep Kits. The DNA library will then undergo a quality check prior to sequencing.

Step 6: Sequencing

We will sequence your libraries on the Illumina MiSeq platform to achieve your required coverage and read depth.

Step 7: Bioinformatics Analysis

Once the sequencing is complete, our team of bioinformaticians conduct a comprehensive analysis to help make sense of your data. The analysis includes taxonomic classification down to the phylum and genus level, Shannon diversity index, OTU (operational taxonomic unit) classification with 97% similarity identity, weighted and unweighted diversity and rooted phylogenetic relationships. You will receive a final report, which will include a summary of your project as well as your data illustrated in ready-to-publish figures.

Sample Report

Let's get your project started.

Request Your Consultation

16S Variable Region Interactive Tool

Using the Interactive Variable Region Diagram, select any of the regions below to learn about which variable region may be best suited for your sequencing project.


Region V1 to V2 (Nucleotides 69-242)

  • Used to distinguish Streptococcus sp. and differentiate between Staphylococcus aureus and coagulase negative Staphylococcus species
  • Able to distinguish among Staphylococcal, Streptococcal, Clostridium, Haemophilus and Neisseria species
  • Best target for distinguishing among Mycobacterium sp.
  • Able to detect Actinomycetaceae, Bacillaceae, Bacteriodaceae, Clostridiaceae, Deinococcaceae, Enterococcaceae, Helicobacteraceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Propionibacteriaceae, Pseudomonadaceae, Rhodobacteraceae, Staphylococcaceae, Streptococcaceae at both the Family and Genus level
  • Able to detect Enterobacteriaceae at the Family level only
  • Not useful for most Escherichia sp., Shigella sp., K. pneumoniae and E. aerogene

Region V1 to V3 (Nucleotides 69-497)

  • Used to distinguish Staphylococci populations
  • Able to detect Prevotella, Porphyromonas, and Bacteroides

Region V1 to V2 (Nucleotides 69-242)

  • Used to distinguish Streptococcus sp. and differentiate between Staphylococcus aureus and coagulase negative Staphylococcus species
  • Able to distinguish among Staphylococcal, Streptococcal, Clostridium, Haemophilus and Neisseria species
  • Best target for distinguishing among Mycobacterium sp.
  • Able to detect Actinomycetaceae, Bacillaceae, Bacteriodaceae, Clostridiaceae, Deinococcaceae, Enterococcaceae, Helicobacteraceae, Lactobacillaceae, Listeriaceae, Moraxellaceae, Neisseriaceae, Propionibacteriaceae, Pseudomonadaceae, Rhodobacteraceae, Staphylococcaceae, Streptococcaceae at both the