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Rapid purification of highly active virus for in vivo and in vitro experiments

  • Isolation of adeno-associated virus (AAV) or adenovirus from any input – cell fraction or media fraction
  • Rapid purification within 2 to 4.5 hours
  • High viral recovery
  • No specialized equipment needed
  • Purification from a variety of viral serotypes
  • Yields highly active virus for use in in vivo and in vitro experiments
  • Purification from a variety of viral serotypes

Recombinant AAV vectors and adenoviral vectors are highly promising tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenoviruses have shown encouraging results for cancer treatment. Norgen’s viral purification kits provide fast and simple procedures for concentrating and purifying AAV and adenoviral vectors from cell lysate and cell culture media. Purification is based on the use of Norgen’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced via enzymatic digestion. Viral vectors purified with these kits are highly active for use in in vitro and in vivo transduction experiments.

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