RNA Sequencing
Total RNA or (mRNA) Sequencing
- Comprehensive Service for Total RNA or mRNA sequencing
- RNA Extraction Service available upon request
- Easy to Challenging Sample Types Including Liquid Biopsies
- Illumina® NextSeq Sequencing Platform
- Receive Complete View of Transcriptome with Bioinformatics Analysis Service
- Quantify Gene Expression and Discover Novel Transcripts
- Fast Turn Around Time | 4 - 6 weeks
In our accredited state-of-the-art laboratory, we offer comprehensive Next-Generation Sequencing (NGS) services to provide full workflow solutions, from RNA isolation and sequencing to advanced bioinformatic analyses for ready-to-publish data. Our experienced staff have extensive expertise working with a variety of sample types, including blood, cells, tissue, urine, plants and more. We are here to help!
Our NGS Clients
We are pleased to work with some of the most prestigious companies and institutions in 155+ countries.
Bioinformatics Analysis
Figure 1. Overview of the sequencing run.
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Figure 2. The normalized rRNA reads relative to raw reads.
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Figure 3. Biotype distribution within an NGS library. (A) Distribution in the entire library and (B) relative biotype distribution within each sample. The graphs are used to analyze biotype fractions within a sample or across all samples. The analysis report contains larger figures.
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Figure 4. Gene abundance distribution. (A) Raw counts or (B) density of genes within each sample are graphed based on the number of genes at each raw count or density. The normal distribution of the number of genes in a sample is reversibly proportional to read counts. The analysis report contains larger figures.
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Figure 5. (Advanced Analysis) Volcano plot illustrating the relation between –log10 FDR and log2 fold change between the control and treatment groups. Highly deregulated genes have a fold change of ≥2 (log2 fold change of ≥1 or ≤-1) while significantly deregulated genes have an FDR of ≤0.05 (-log10 FDR of ≥1.30103).
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Figure 6. (Advanced Analysis) Principal component analysis (PCA) plot. The plot was generated by using 50 genes that have the highest %CV.
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Figure 7. (Advanced Analysis) Heat map with hierarchal clustering of genes and samples. The graph was generated by using 50 genes that have the highest %CV. The color scale indicates the relative expression level of a gene to the mean, where green and red indicate higher or lower expression, respectively.
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Figure 8. (Advanced Analysis) Bubble plot displaying GO terms determined to be significantly enriched within the up-regulated differentially expressed genes. GO terms that are significantly enriched are displayed as their GO:ID on the plot, but their associated term is listed and grouped by their respective domains.
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Figure 9. (Advanced Analysis) Barplot listing the gene counts of the top 20 enriched GO terms from each domain (BP, MF, CC) organized by p-value.
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Your Custom Workflow
1. Consultation
2. Sample Submission/Shipping
3. Sample Isolation
4. RNA Quality Control
5. Library Preparation
6. Sequencing
7. Bioinformatics Analysis
8. Final Report
Step 1: Consultation
Get started by requesting your free consultation with one of our NGS experts. We would love to discuss your research objectives and are happy to provide assistance in defining your project goals. Our NGS specialists and bioinformaticians are here to provide customized recommendations to suit your project needs.
Request Your Consultation
Step 2: Sample Submission & Shipping
Shipping your samples to our facility is easy and painless! We'll provide you with detailed instructions on how to handle, process and ship your samples to our laboratory. We accept both purified RNA and specimens for RNA isolation services.
If you are shipping specimens to us, a complete guide on sample collection and preservation will be provided to you. Recommended specimen input and handling procedures for RNA purification can be found in our RNA-Seq Service Guide.
How will you submit your samples?
For detailed shipping instructions, please refer to the document below.
Shipping Instructions
Step 3: Sample Isolation
Our team has tremendous experience and expertise in isolating RNA from any sample type including, cell lines, tissues, bodily fluids and environmental samples. Utilizing our proprietary silicon carbide resin for all isolations allows us to capture total RNA with greater diversity. RNA isolation is included in your RNA Sequencing service.
Step 4: RNA Quality Control
You can be confident that the sequencing portion of your project will be successful as we perform quality control on the isolated RNA to assess quantity, quality and amplifiability prior to library preparation.
Step 5: Library Preparation
Our team has expertise in RNA library preparation from all types of specimens. We will prepare the RNA-Seq libraries based on your consultation, utilizing either Poly A enrichment or rRNA depletion methods. The RNA library will then undergo a quality check prior to sequencing.
Step 6: Sequencing
We will sequence the sample libraries on the Illumina® NextSeq platform to achieve your required coverage and read depth.
Step 7: Bioinformatics Analysis
Once the sequencing is complete, our team of bioinformaticians conduct a comprehensive analysis to help make sense of your data.
Customized advanced analyses, such as Differential Expression and KEGG/GO enrichment can be added to the service to answer a specific biological question. You will receive a final report, which will include a summary of your project as well as your data illustrated in ready-to-publish figures.
Let's get your project started.
Request Your ConsultationQuality In → Quality Out
We believe that the best research starts with quality sample collection and preservation.
Saliva RNA Collection and Preservation Devices Dx
(Cat. 53800)
CE-IVD marked in accordance with EU Directive 98/79/EC
Total Nucleic Acid Preservation Tubes Dx
(Cat. Dx69200)
CE-IVD marked in accordance with EU Directive 98/79/EC
Stool Nucleic Acid Collection and Preservation Devices Dx
(Cat. Dx45660)
CE-IVD marked in accordance with EU Directive 98/79/EC
Stool Nucleic Acid Collection and Preservation System
(Cat. 63700)
For Research Use
Fecal Swab Collection and Preservation System
(Cat. 45670)
For Research Use
Urine Preservation
(Cat. 18116, 18118, 18120)
For Research Use
Urine Collection and Preservation Cup
(Cat. 18129)
For Research Use
cf-DNA/cf-RNA Preservative Tubes Dx
(Cat. Dx63950)
CE-IVD marked in accordance with EU Directive 98/79/EC
Frequently Asked Questions
What is Next Generation Sequencing?
Next Generation Sequencing (NGS) is an important tool used in the field of genomic research as it allows for high throughput and massively parallel sequencing of thousands of molecules simultaneously. NGS provides researchers with fast, scalable solutions for a clear, complete picture of their samples to advance their scientific discoveries.
What is bioinformatics?
Bioinformatics has quickly advanced to become an important and powerful application in biological research. It involves a combination of computational and statistical methods to better understand and analyze scientific data, guiding the research community to new opportunities and novel discoveries. As an emerging discipline, bioinformatics continues to be an essential tool for researchers in many fields including modern molecular biology and clinical research, with its applications leading to significant medical advancements and developments in future research to come.
Do you offer extraction services?
Yes. RNA extraction service can be included for any RNA-Seq project at an additional cost.
What are your sample submission requirements?
Our requirements for sample submission can be found under Step 2: Sample Submission on our RNA NGS Service page.
What sequencing platform do you sequence on?
We sequence all RNA Sequencing projects on the Illumina® NextSeq platform.
What is the depth of coverage Norgen provides?
Our standard depth is 20 Million (Paired End)(average) raw reads per sample. We can accommodate higher or lower read depth if required. Please contact services@norgenbiotek.com to discuss the optimal read depth for your project goals.
Does the price include bioinformatics analysis?
Yes. Our comprehensive service includes a basic bioinformatics analysis of your raw data. We deliver the bioinformatics analysis in a PDF report and we also provide you with a secure link to download all of your raw data and intermediate files. In order to get the advanced analysis for RNA Sequencing, there is an additional fee.
What is the typical turnaround time?
Our typical turnaround time is 3-4 weeks from the time of sample receipt. The turnaround time is dependent on the size of your project. Please contact services@norgenbiotek.com to receive an accurate estimated turnaround time.
Norgen is located in Canada. Do you still accept samples from other countries?
Absolutely! We accept samples from countries worldwide and our NGS team will be happy to assist you with making the shipping arrangements.
Citations
| Title | Alteration of miRNAs in Small Neuron‑Derived Extracellular Vesicles of Alzheimer's Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses |
| Journal | Journal of Molecular Neuroscience . 2022. |
| Authors | Devrim Yagmur Durur, Bora Tastan, Kemal Ugur Tufekci, Melis Olcum, Hamdiye Uzuner, Gökhan Karakülah, Gorsev Yener, Sermin Genc |