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Next Generation Sequencing Services

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RNA (mRNA) Sequencing

  • Comprehensive Service in an Illumina Propel Certified Laboratory
  • Various Sample Types
  • Illumina® NextSeq Sequencing Platform
  • Capture and Identify Complete View of the Coding Transcriptome
  • Quantify Gene Expression
  • Discover Novel Transcript Features
  • Fast Turn Around Time

Norgen Biotek is an Illumina Propel-Certified Sequencing facility and offers Next-Generation Sequencing (NGS) in an accredited state-of-the-art laboratory. Our comprehensive service includes sample isolation to library prep and sequencing to full bioinformatics analysis. We have extensive expertise in sample preparation, sequencing and analysis of all types of samples.


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Our Comprehensive Workflow


Step 1: Consultation

It is essential to have an initial engagement regarding your sequencing project with our experts to define and set clear objectives. We will discuss with you various elements of your project to learn how we can best achieve your goals. Our NGS scientists and bioinformaticians will be happy to learn about your project and make the best recommendations to suit your project needs.

Please contact us to schedule your complimentary consultation.

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Step 2a: Sample Submission

We will provide detailed instructions on how to handle, process and ship your samples to our facility. We accept both purified RNA and specimens for RNA isolation services. In addition, we offer a complete guideline on sample collection and preservation. Recommended specimen input and handling precautions for RNA purification can be found in our RNA-Seq Service Guide.

How will you submit your samples?

  1. I still need to collect my samples
  2. I will send specimens
  3. I will send purified RNA
Sample Submission

Step 2b: Shipping

For detailed shipping instructions, please refer to the document below.

Shipping Instructions
Shipping

Step 3: Sample Isolation

We have tremendous experience and expertise in isolating RNA from any sample type including, cell lines, tissues, bodily fluids and environmental samples. Our dedicated lab technicians utilize our proprietary silicon carbide resin for all isolations which allows us to capture total RNA with greater diversity.

Total RNA Purification Kit

Step 4: RNA Quality Control

We perform quality control on the isolated RNA to assess quantity, quality, and amplifiability prior to library prep. We can share this data prior to proceeding with sequencing at your request.

Total RNA Purification Kit

Step 5: Library Preparation

We have expertise in RNA library preparation from all types of specimens. We will prepare the RNA-Seq libraries based on your consultation utilizing either Poly A enrichment or rRNA depletion methods. The RNA library will then undergo QC prior to sequencing.

Small RNA Library Prep Kit

Step 6: Sequencing

We will sequence the sample libraries on the Illumina platform to achieve your required coverage and read depth.


Total RNA Purification Kit

Step 7: Analysis

We have full bioinformatics capabilities from basic analysis to advanced analysis pipelines to help make sense of your data. All of our RNA-Seq services include basic bioinformatics analysis as a deliverable in your final report. Analysis can be customized to include advanced analyses such as Differential Expression and KEGG/GO enrichment to answer a specific biological question.

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Final Report


Let's get your project started.

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