Small RNA Sequencing

Small RNA (microRNA) Sequencing

  • Capture a Complete Range of Small RNA and miRNA species
  • Sequence Ultra-Low Inputs from Liquid Biopsies and Exosomes
  • Illumina® NextSeq Sequencing Platform
  • Understand Post-Transcriptional Regulation with Bioinformatics Analysis Service
  • Examine Differential Expression and Discover Novel Biomarkers
  • Fast Turn Around Time | 3 - 4 weeks

In our accredited state-of-the-art laboratory, we offer comprehensive Next-Generation Sequencing (NGS) services to provide full workflow solutions, from RNA isolation and sequencing to advanced bioinformatic analyses for ready-to-publish data. Our experienced staff have extensive expertise working with a variety of sample types, including, urine, tissue, exosomes, plasma/serum, saliva, FFPE, cerebral spinal fluid (CSF) and more. We are here to help!

Next Generation Sequencing

Bioinformatics Analysis

Your Custom Workflow


Step 1: Consultation

Get started by booking your free consultation with one of our metagenomics experts. We would love to discuss your research objectives and are happy to provide assistance in defining your project goals. Our NGS specialists and bioinformaticians are here to provide customized recommendations to suit your project needs.

Book Your Consultation

Step 2: Sample Submission & Shipping

Shipping your samples to our facility is easy and painless! We’ll provide you with detailed instructions on how to handle, process and ship your samples to our laboratory. We accept both purified RNA and specimens for RNA isolation services.

If you are shipping specimens to us, a complete guide on sample collection and preservation will be provided to you. Recommended specimen input and handling procedures for RNA purification can be found in our Small RNA-Seq Service Guide.

How will you submit your samples?

  1. I still need to collect my samples
  2. I will send specimens
  3. I will send purified RNA

For detailed shipping instructions, please refer to the document below.

Shipping Instructions

Step 3: Sample Isolation

Our team has tremendous experience and expertise in isolating RNA from ultra-low concentration samples such as liquid biopsies (plasma, serum, urine, CSF), exosomes and FFPE. Utilizing our proprietary silicon carbide resin for all isolations allows us to capture miRNA with greater diversity and without the use of phenol.

Step 4: RNA Quality Control

You can be confident that the sequencing portion of your project will be successful as we perform quality control on the isolated RNA to assess quantity, quality and amplifiability prior to library preparation.

Step 5: Library Preparation

Our team has expertise in RNA library preparation from all types of specimens, successfully generating libraries from ultra-low RNA inputs. We will generate the small RNA libraries using Norgen Biotek’s Small RNA Library Preparation Kit (for Illumina). The libraries will then undergo a quality check prior to sequencing.

Step 6: Sequencing

We will sequence the sample libraries on the Illumina® NextSeq platform to achieve your required coverage and read depth.

Step 7: Bioinformatics Analysis

Once the sequencing is complete, our team of bioinformaticians conduct a comprehensive analysis to help make sense of your data. Customized advanced analyses, such as Differential Expression, KEGG/GO and Novel miRNA Discovery can be added to the service to answer a specific biological question. You will receive a final report, which will include a summary of your project as well as your data illustrated in ready-to-publish figures.

Sample Report

Let's get your project started.

Book Your Consultation

Quality In → Quality Out

We believe that the best research starts with quality sample collection and preservation.

Select your sample type: Saliva/Swab Stool/Swab Urine Blood
Saliva RNA Collection and Preservation Devices Dx (Cat. 53800)
CE-IVD marked in accordance with EU Directive 98/79/EC
Total Nucleic Acid Preservation Tubes Dx (Cat. Dx69200)
CE-IVD marked in accordance with EU Directive 98/79/EC
Stool Nucleic Acid Collection and Preservation Devices Dx (Cat. Dx45660)
CE-IVD marked in accordance with EU Directive 98/79/EC
Stool Nucleic Acid Collection and Preservation System (Cat. 63700)
For Research Use
Fecal Swab Collection and Preservation System (Cat. 45670)
For Research Use
Urine Preservation (Cat. 18116, 18118, 18120)
For Research Use
Urine Collection and Preservation Cup (Cat. 18129)
For Research Use
cf-DNA/cf-RNA Preservative Tubes Dx (Cat. Dx63950)
CE-IVD marked in accordance with EU Directive 98/79/EC

Frequently Asked Questions

What is Next Generation Sequencing?

Next Generation Sequencing (NGS) is an important tool used in the field of genomic research as it allows for high throughput and massively parallel sequencing of thousands of molecules simultaneously. NGS provides researchers with fast, scalable solutions for a clear, complete picture of their samples to advance their scientific discoveries.

What is bioinformatics?

Bioinformatics has quickly advanced to become an important and powerful application in biological research. It involves a combination of computational and statistical methods to better understand and analyze scientific data, guiding the research community to new opportunities and novel discoveries. As an emerging discipline, bioinformatics continues to be an essential tool for researchers in many fields including modern molecular biology and clinical research, with its applications leading to significant medical advancements and developments in future research to come.

Do you offer extraction services?

Yes. RNA extraction service can be included for any RNA-Seq project at an additional cost.

What are your sample submission requirements?

Our requirements for sample submission can be found under Step 2: Sample Submission on our small RNA NGS Service page.

What sequencing platform do you sequence on?

We sequence all RNA Sequencing projects on the Illumina® NextSeq platform.

What is the depth of coverage Norgen provides?

Our standard depth is 10 Million (average) raw reads per sample. We can accommodate higher or lower read depth if required. Please contact services@norgenbiotek.com to discuss the optimal read depth for your project goals.

Does the price include bioinformatics analysis?

Yes. Our comprehensive service includes a basic bioinformatics analysis of your raw data. We deliver the bioinformatics analysis in a PDF report and we also provide you with a secure link to download all of your raw data (fastq) and intermediate files. In order to get the advanced analysis for Small RNA Sequencing, there is an additional fee.

What is the typical turnaround time?

Our typical turnaround time is 3-4 weeks from the time of sample receipt. The turnaround time is dependent on the size of your project. Please contact services@norgenbiotek.com to receive an accurate estimated turnaround time.

Norgen is located in Canada. Do you still accept samples from other countries?

Absolutely! We accept samples from countries worldwide and our NGS team will be happy to assist you with making the shipping arrangements.

Our NGS Clients

We are pleased to work with some of the most prestigious companies and institutions in 155+ countries.