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Norgen Biotek is an Illumina Propel-Certified Sequencing facility and offers Next-Generation Sequencing (NGS) in an accredited state-of-the-art laboratory. Our comprehensive service includes sample isolation to library prep and sequencing to full bioinformatics analysis. We have extensive expertise in sample preparation, sequencing and analysis of all types of samples.
Figure 1. Graph illustrating that the relative expression level of each microRNA detected is highly correlated between 50µL and 4mL of plasma.
Figure 1. Graph illustrating that the relative expression level of each microRNA detected is highly correlated between 50µL and 4mL of plasma.
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Figure 2. Graph illustrating that the relative expression level of each microRNA detected is highly correlated between 200µL and 4mL of plasma.
Figure 2. Graph illustrating that the relative expression level of each microRNA detected is highly correlated between 200µL and 4mL of plasma.
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Figure 3.(Advanced Analysis) Bubble plot illustrating GO terms determined to be significantly enriched within the up-regulated differentially expressed genes. GO terms that are significantly enriched are displayed as their GO:ID on the plot, but their associated term is listed and grouped by their respective domains.
Figure 3.(Advanced Analysis) Bubble plot illustrating GO terms determined to be significantly enriched within the up-regulated differentially expressed genes. GO terms that are significantly enriched are displayed as their GO:ID on the plot, but their associated term is listed and grouped by their respective domains.
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Figure 4.(Advanced Analysis) Heat map displaying the hierarchal clustering of miRNAs and samples based on their expression levels. The graph was generated by using 50 miRNAs that have the highest %CV. The color scale indicates the relative expression level of a miRNA to the mean, where green and red indicate higher or lower expression, respectively.
Figure 4.(Advanced Analysis) Heat map displaying the hierarchal clustering of miRNAs and samples based on their expression levels. The graph was generated by using 50 miRNAs that have the highest %CV. The color scale indicates the relative expression level of a miRNA to the mean, where green and red indicate higher or lower expression, respectively.
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Figure 5.(Advanced Analysis)PCA (Principle Component Analysis) illustrating the distance between samples based on their miRNA profiles. The plot was generated by using 50 miRNAs which show the highest variation across all samples, calculated by the %CV (coefficient of variation).
Figure 5.(Advanced Analysis)PCA (Principle Component Analysis) illustrating the distance between samples based on their miRNA profiles. The plot was generated by using 50 miRNAs which show the highest variation across all samples, calculated by the %CV (coefficient of variation).
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Figure 6.Graph illustrating the number of microRNAs detected from 50 µL, 200 µL and 4mL of plasma. The numbers of miRNAs from varying volumes do not differ significantly.
Figure 6.Graph illustrating the number of microRNAs detected from 50 µL, 200 µL and 4mL of plasma. The numbers of miRNAs from varying volumes do not differ significantly.
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Figure 7.Graph showing different small RNA biotype fractions within each sample as well as between sample relatively to the number of mapped reads.
Figure 7.Graph showing different small RNA biotype fractions within each sample as well as between sample relatively to the number of mapped reads.
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Figure 8.(Advanced Analysis) Venn diagram showing the different numbers of miRNA detected in different volumes of plasma. The diagram illustrates that of the microRNAs identified, the majority are detected in all volumes of plasma input from 50 μL to 4 mL
Figure 8.(Advanced Analysis) Venn diagram showing the different numbers of miRNA detected in different volumes of plasma. The diagram illustrates that of the microRNAs identified, the majority are detected in all volumes of plasma input from 50 μL to 4 mL
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Figure 9. Volcano plot illustrating the relation between –log10 FDR and log2 fold change between the control and treatment groups. Highly deregulated miRNAs have a fold change of ≥2 (log2 fold change of ≥1 or ≤-1) while significantly deregulated miRNAs have an FDR of ≤0.05 (-log10 FDR of ≥1.30103).
Figure 9. Volcano plot illustrating the relation between –log10 FDR and log2 fold change between the control and treatment groups. Highly deregulated miRNAs have a fold change of ≥2 (log2 fold change of ≥1 or ≤-1) while significantly deregulated miRNAs have an FDR of ≤0.05 (-log10 FDR of ≥1.30103).
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1. Consultation 2. Sample Submission/Shipping 3. Sample Isolation 4. RNA Quality Control
5. Library Preparation 6. Sequencing 7. Analysis 8. Final Report
It is essential to have an initial engagement regarding your sequencing project with our experts to define and set clear objectives. We will discuss with you various elements of your project to learn how we can best achieve your goals. Our NGS scientists and bioinformaticians will be happy to learn about your project and make the best recommendations to suit your project needs.
Please contact us to schedule your complimentary consultation.
We will provide detailed instructions on how to handle, process and ship your samples to our facility. We accept both purified RNA and specimens for RNA isolation services. In addition, we offer a complete guideline on sample collection and preservation. Recommended specimen input and handling precautions for RNA purification can be found in our Small RNA-Seq Service Guide.
How will you submit your samples?
For detailed shipping instructions, please refer to the document below.
We have tremendous experience and expertise in isolating RNA from ultra-low concentration samples such as liquid biopsies (plasma, serum, urine, CSF), exosomes and FFPE. Our dedicated lab technicians utilize our proprietary silicon carbide resin for all isolations which allows us to capture miRNA with greater diversity and without the use of phenol. We accept any specimen for RNA isolation, including cell lines, tissues, bodily fluids and environmental samples.
We perform quality control on the isolated RNA to assess quantity, quality, and amplifiability prior to library prep. We can share this data prior to proceeding with sequencing at your request.
We generate small RNA libraries using Norgen Biotek’s Small RNA Library Preparation Kit (for Illumina). We have expertise in RNA library preparation from all types of specimens and successfully generate libraries from ultra-low RNA inputs.
We will sequence the sample libraries on the Illumina platform to achieve your required coverage and read depth.
We have full bioinformatics capabilities from basic analysis to advanced analysis pipelines to help make sense of your data. All our Small RNA-Seq services include basic bioinformatics analysis as a deliverable in your final report. Analysis can be customized to include advanced analyses such as Differential Expression, KEGG/GO and Novel miRNA Discovery to answer a specific biological question.
| Title | Circulating MicroRNAs and Treatment Response in Childhood Asthma |
| Journal | ATS Jounals. 2020. |
| Authors | Jiang Li , Ronald Panganiban , Alvin T. Kho , Michael J McGeachie , Leanna Farnam , Robert P. Chase , Scott T Weiss , Quan Lu , and Kelan G Tantisira |
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