Saliva Collection and Preservation Devices
Select each molecule to learn more about Norgen's unique tube and how it separates DNA/RNA from saliva samples
Saliva RNA Collection and Preservation Devices



Saliva RNA Collection and Preservation Devices
Norgen’s Saliva RNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of RNA in saliva samples at ambient temperature. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies.


RNA
Saliva is a source of both cellular and cell-free host RNA as well as exogenous RNA from bacterial or viral cells. The majority of host cellular RNA originates from the buccal mucosa and can be used for transcriptomic analyses to identify which functional pathways are active within the cells. Host cell-free RNA in the saliva is typically introduced into the saliva via filtration from the bloodstream into the salivary glands. While RNA is usually degraded by nucleases in the bloodstream, fragments that are bound to proteins (i.e. ribosomes and other proteins) or encapsulated inside exosomes are protected from degradation and remain in circulation. Once purified, cell-free RNA can be analyzed and used as a biomarker for various diseases and conditions.
Links
Bacteria
Bacteria in saliva are part of the oral microbiome which consists of approximately 700 different species that exist in a symbiotic relationship with the host. A disruption in this relationship, known as dysbiosis, can lead to various oral diseases or systemic diseases. Furthermore, oral dysbiosis is also associated with certain head and neck cancer development. Therefore, identifying changes in salivary bacterial composition can not only be used to diagnose certain oral or systemic diseases but also to understand the role it plays in the alteration of functional and metabolic pathways associated with the development of certain head and neck cancers.
Viruses
Saliva can not only contain viable intact viruses but also viral nucleic acids that can be used to screen for several diseases. Examples of such viruses include Influenza virus A, SARS-CoV-2, Herpes simplex virus 1 and 2, Rabies virus, Epstein-Barr virus, Cytomegalovirus, Rhinoviruses, Hepatitis A, B & C viruses, Measles virus, and Human papillomavirus. Being non-invasive and easy to collect, saliva, therefore, is a valuable specimen to detect viral infections.
Links
Saliva DNA Collection and Preservation Devices



Saliva DNA Collection and Preservation Devices
Norgen's Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen's Saliva DNA Isolation Kit (Cat# RU45400). Each of Norgen's Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.

Cellular g-DNA
The majority of DNA in saliva originates from the buccal epithelial cells shed from the inner lining of the mouth and white blood cells. Also present in the saliva is cell-free DNA that originates from the apoptosis or necrosis of host cells and is usually present in very low concentrations. A clinically important type of cell-free DNA is circulating tumor DNA or ct-DNA which originates from tumor cells. By examining the mutations within the ct-DNA, or its methylation profile, ct-DNA can act as a key biomarker for the diagnosis and monitoring of cancer development. Another important source of DNA in the saliva is bacterial cells inhabiting the oral cavity. This DNA can not only be used to profile the salivary microbiome but is also important in detecting pathogens that cause oral diseases.
Links
Bacteria
Bacteria in saliva are part of the oral microbiome which consists of approximately 700 different species that exist in a symbiotic relationship with the host. A disruption in this relationship, known as dysbiosis, can lead to various oral diseases or systemic diseases. Furthermore, oral dysbiosis is also associated with certain head and neck cancer development. Therefore, identifying changes in salivary bacterial composition can not only be used to diagnose certain oral or systemic diseases but also to understand the role it plays in the alteration of functional and metabolic pathways associated with the development of certain head and neck cancers.
Links
Viruses
Saliva can not only contain viable intact viruses but also viral nucleic acids that can be used to screen for several diseases. Examples of such viruses include Influenza virus A, SARS-CoV-2, Herpes simplex virus 1 and 2, Rabies virus, Epstein-Barr virus, Cytomegalovirus, Rhinoviruses, Hepatitis A, B & C viruses, Measles virus, and Human papillomavirus. Being non-invasive and easy to collect, saliva, therefore, is a valuable specimen to detect viral infections.
Features & Benefits
- Preserved RNA is stable for up to 2 months at room temperature
- Sample collection and preservation in one convenient kit
- Samples are non-infectious and can be handled and shipped safely at ambient temperature
- Non-invasive, user-friendly collection and suitable for self collection
- Hassle-free and cost/effective - no cold shipping/storage needed
- Compatible with RNA isolation methods
- Preserve high-quality RNA that is compatible with any downstream application
- CE-IVD marked version available
- Preserved DNA is stable for 2 years at ambient temperature
- Non-invasive, user-friendly collection and suitable for self collection
- Sample collection and preservation in one convenient kit
- Samples are non-infectious and can be handled and shipped safely at ambient temperature
- Hassle-free and cost/effective - no cold shipping/storage needed
- Isolate high-quality nucleic acids for any downstream applications
- CE-IVD marked version available