Milk DNA Preservation and Isolation Kit (Cat. 44800)

Milk DNA Preservation and Isolation Kit

Cat. 44800

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Milk DNA Preservation and Isolation Kit

Cat. 44800

A rapid all-in-one procedure for the preservation and isolation of milk DNA at ambient temperatures.

Milk samples are stable for 1 month at room temperature (or 1 week at 37°C) in the Preservation Solution
Fast and easy processing using a rapid spin-column format
DNA can be isolated and detected from as little as 100 µL of milk
Isolate high quality genomic DNA

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Milk DNA Preservation and Isolation Kit
(Cat. 44800) 25 preps

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 44800

Kit Specifications
Maximum Milk Input	0.5 mL
Time to Complete 10 Purifications	80 minutes (20 minutes hands on)

Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Storage in Preservation Buffer
1) Storage at -20°C is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -70°C.
2) Samples can be stored at room temperature (25°C) for up to 1 month without significant loss of DNA quality.
3) DNA has also been successfully isolated from samples stored at 37°C 1 for one week.

Supporting Data

Figure 1. Isolation of DNA From Different Volumes of PreservedMilk(0.5 mL, 1 mL, 1.5 mL and 2 mL) Using Norgens Milk DNA Preservation and Isolation Kit. For evaluation, ten microlitres of the isolated 100 µL DNA was loaded on 1.2% agarose gel, and all DNA samples on the gel showed good integrity (A). The correlated DNA yield increase (B) with increasing preserved milk input volumes,along with the gel analysis, indicates that Norgens Milk DNA Preservation and Isolation Kit (Cat. 44800) was able to isolate milk DNA from up to 2 mL preserved milk. Lane M is Norgen's UltraRanger DNA Ladder (Cat. 12100).

Figure 2. Amplification of 16s rRNA Gene From Different Volumes of Preserved Milk. E.coli was spiked into the preserved milk and the DNA was then isolated using Norgen's Milk DNA Preservation and Isolation Kit (Cat. 44800). Two microlitres of the 100 µL isolated DNA were used in a 20 µL qPCR reaction using TaqMan 16s rRNA qPCR. No PCR inhibition was observed from all milk DNA isolated from the different preserved milk volumes (Pink: 0.5 mL, Blue: 1 mL, Red: 1.5 mL and Green: 2 mL), indicating the high quality of milk DNA.

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Component	Cat. 44800 (25 preps)
Preservation Solution E	30 mL
Proteinase K	0.6 mL
Purification Additive	0.5 mL
Buffer SK	40 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Mini Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1

Protocols:

(44800) Milk DNA Preservation and Isolation Kit - Protocol (25 prep)

Product Information Sheets:

Milk DNA Preservation and Isolation Kit - Product Information Sheet

Safety Data Sheets:

(44800) Milk DNA Preservation and Isolation Kit - Safety Data Sheet

Title	Comparison of commercially-available preservatives for maintaining the integrity of bacterial DNA in human milk
Journal	Journal of Microbiological Methods. 2017.
Authors	Kimberly A. Lackey, Janet E. Williams, William J. Price, Janae M. Carrothers, Sarah L. Brooker, Bahman Shafii, Mark A. McGuire, Michelle K. McGuire

Title	Real-Time PCR Detection of Paenibacillus spp. in Raw Milk to Predict Shelf-Life Performance of Pasteurized Fluid Milk Products.
Journal	Applied and Environmental Microbiology. 2012.
Authors	Ranieri ML, Ivy RA, Mitchell WR, Call E, Masiello SN, Wiedmann M, Boor KJ.

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Milk DNA Preservation and Isolation Kit

Norgen’s Milk DNA Preservation and Isolation Kit is an all-in-one solution designed for 1) preservation of DNA in milk samples at ambient temperature; and 2) isolation of high quality DNA within a laboratory setting. The kit is provided with a Preservation Solution that allows for either immediate processing of milk samples, or allows for the storage of the milk samples for up to 1 month at room temperature prior to DNA isolation. Genomic DNA extracted from somatic cells and bacteria found in milk can be used in various applications. The isolated DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Milk DNA can also be used to diagnose particular types of infections. Milk DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Milk DNA Preservative

Norgen’s Milk DNA Preservation Solution is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the preservative allow samples to be stored for 1 month without any detectable DNA degradation.

Supporting Data

Figure 1. Isolation of DNA From Different Volumes of PreservedMilk(0.5 mL, 1 mL, 1.5 mL and 2 mL) Using Norgens Milk DNA Preservation and Isolation Kit. For evaluation, ten microlitres of the isolated 100 µL DNA was loaded on 1.2% agarose gel, and all DNA samples on the gel showed good integrity (A). The correlated DNA yield increase (B) with increasing preserved milk input volumes,along with the gel analysis, indicates that Norgens Milk DNA Preservation and Isolation Kit (Cat. 44800) was able to isolate milk DNA from up to 2 mL preserved milk. Lane M is Norgen's UltraRanger DNA Ladder (Cat. 12100).

Figure 2. Amplification of 16s rRNA Gene From Different Volumes of Preserved Milk. E.coli was spiked into the preserved milk and the DNA was then isolated using Norgen's Milk DNA Preservation and Isolation Kit (Cat. 44800). Two microlitres of the 100 µL isolated DNA were used in a 20 µL qPCR reaction using TaqMan 16s rRNA qPCR. No PCR inhibition was observed from all milk DNA isolated from the different preserved milk volumes (Pink: 0.5 mL, Blue: 1 mL, Red: 1.5 mL and Green: 2 mL), indicating the high quality of milk DNA.

Click for expanded view

Kit Specifications

You have selected: Cat. 44800

Kit Specifications
Maximum Milk Input	0.5 mL
Time to Complete 10 Purifications	80 minutes (20 minutes hands on)

Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Storage in Preservation Buffer
1) Storage at -20°C is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -70°C.
2) Samples can be stored at room temperature (25°C) for up to 1 month without significant loss of DNA quality.
3) DNA has also been successfully isolated from samples stored at 37°C 1 for one week.

Component	Cat. 44800 (25 preps)
Preservation Solution E	30 mL
Proteinase K	0.6 mL
Purification Additive	0.5 mL
Buffer SK	40 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Mini Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1

Protocols:

(44800) Milk DNA Preservation and Isolation Kit - Protocol (25 prep)

Product Information Sheets:

Milk DNA Preservation and Isolation Kit - Product Information Sheet

Safety Data Sheets:

(44800) Milk DNA Preservation and Isolation Kit - Safety Data Sheet

Title	Comparison of commercially-available preservatives for maintaining the integrity of bacterial DNA in human milk
Journal	Journal of Microbiological Methods. 2017.
Authors	Kimberly A. Lackey, Janet E. Williams, William J. Price, Janae M. Carrothers, Sarah L. Brooker, Bahman Shafii, Mark A. McGuire, Michelle K. McGuire

Title	Real-Time PCR Detection of Paenibacillus spp. in Raw Milk to Predict Shelf-Life Performance of Pasteurized Fluid Milk Products.
Journal	Applied and Environmental Microbiology. 2012.
Authors	Ranieri ML, Ivy RA, Mitchell WR, Call E, Masiello SN, Wiedmann M, Boor KJ.

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Milk DNA Preservation and Isolation Kit (Cat. 44800)

Milk DNA Preservation and Isolation Kit

Milk DNA Preservation and Isolation Kit

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Related Products

Milk Bacterial DNA Isolation Kit

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Milk DNA Preservation and Isolation Kit (Cat. 44800)

Milk DNA Preservation and Isolation Kit

Milk DNA Preservation and Isolation Kit

Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Product Overview

Documentation

Citations

Related Products

Milk Bacterial DNA Isolation Kit

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.