A rapid all-in-one procedure for the preservation and isolation of milk DNA at ambient temperatures.
|Milk DNA Preservation and Isolation Kit||44800||25 preps|
Milk DNA Preservation and Isolation Kit
Norgen’s Milk DNA Preservation and Isolation Kit is an all-in-one solution designed for 1) preservation of DNA in milk samples at ambient temperature; and 2) isolation of high quality DNA within a laboratory setting. The kit is provided with a Preservation Solution that allows for either immediate processing of milk samples, or allows for the storage of the milk samples for up to 1 month at room temperature prior to DNA isolation. Genomic DNA extracted from somatic cells and bacteria found in milk can be used in various applications. The isolated DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Milk DNA can also be used to diagnose particular types of infections. Milk DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Milk DNA Preservative
Norgen’s Milk DNA Preservation Solution is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the preservative allow samples to be stored for 1 month without any detectable DNA degradation.
Maximum Milk Input
Time to Complete 10 Purifications
(20 minutes hands on)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The Proteinase K can be stored either at room temperature or 4°C.
Storage in Preservation Buffer
1) Storage at -20°C is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -70°C.
2) Samples can be stored at room temperature (25°C) for up to 1 month without significant loss of DNA quality.
3) DNA has also been successfully isolated from samples stored at 37°C 1 for one week.
|Title||Comparison of commercially-available methods to preserve DNA in human milk (623.10)|
|Journal||The FASEB Journal. 2014.|
|Authors||Kimberly Lackey, Janet Williams, Janae Mosely, Sarah Brooker, Mark McGuire and Michelle McGuire.|
|Title||Real-Time PCR Detection of Paenibacillus spp. in Raw Milk to Predict Shelf-Life Performance of Pasteurized Fluid Milk Products.|
|Journal||Applied and Environmental Microbiology. 2012.|
|Authors||Ranieri ML, Ivy RA, Mitchell WR, Call E, Masiello SN, Wiedmann M, Boor KJ.|
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