For rapid and efficient endotoxin removal from proteins and peptides

  • Columns bind proteins of interest while endotoxins flow through
  • Reduce endotoxin levels to less than 0.1 EU/µg of protein
  • Greater than 95% protein recovery
  • High protein recovery with excellent endotoxin reduction
  • Concentrate protein samples and remove detergents, salts and denaturants at the same time
  • Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20

ItemsCat. #Size
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit 22200 4 preps

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is also highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is also designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. This kit efficiently reduces endotoxin levels to ≤ 0.1 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes

Kit Specifications
Maximum Input Volume
4 mg
Minimum Protein Volume Input
0.25 mg
Maximum Column Volume Input 18 mL
Molecular Weight of Recovered Proteins No molecular weight cut-off
Final Endotoxin Levels
≤ 0.01 EU/μg protein
Protein Recovery
90-95%
% Detergent Removal 90-95%
Detergents that can be removed Including Triton® X-100, CHAPS, NP-40 and Tween 20
Elution Volume 2-4 mL
Time to Complete 10 Purifications
30-40 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 22200 (4 preps)
Binding Buffer J 8 mL
Binding Buffer N 20 mL
Wash Solution M 130 mL
Wash Solution CIP 60 mL
Wash Solution N 130 mL
Wash Solution NIP 60 mL
Elution Buffer G 20 mL
Endotoxin Removal Solution 1.5 mL
Protein Neutralizer EF 2 mL
Maxi Spin Columns (assembled with Collection Tubes) 4
Elution Tubes (50 mL) 4
Product Insert 1

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