军团菌TaqMan 聚合酶链式反应检测试剂盒
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Supporting Data
Figure 1. TaqMan PCR Kit.
Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.
Figure 2. TaqMan PCR Kit.
Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.
Figure 3. TaqMan PCR Kit.
Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.
TaqMan 聚合酶链式反应试剂盒 -图 1.TaqMan 聚合酶链式反应阳性结果示例。FAM 和 HEX 通道的聚合酶链式反应信号均高于基线,表明聚合酶链式反应成功。
TaqMan 聚合酶链式反应试剂盒 - 图 2.TaqMan 聚合酶链式反应阴性结果示例。FAM 通道未检测到目标 DNA,但 HEX 通道的扩增信号表明聚合酶链式反应成功。
TaqMan 聚合酶链式反应试剂盒 - 图 3.TaqMan 聚合酶链式反应抑制结果示例。FAM 和 HEX 通道均未检测到信号。建议使用推荐的 DNA 纯化试剂盒重复样本制备。
储存条件和产品稳定性
所有试剂盒组件可在生产日期后存放 2 年,而不会降低性能。
所有试剂盒组件到达后应存放在 -20°C 温度下。应避免重复解冻和冷冻(> 2 次)混合母液和阳性对照,因为这可能会影响检测的性能。如果试剂只能间歇使用,则应等分冷冻。
Documentation
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