Para una purificación rápida del ARN total, incluido el microARN, sin el uso de fenol
Supporting Data
Figure 1. Isolate a Diversity of RNA Species.
Total RNA was isolated from 10 mL of an overnight E. coli culture using Norgen's Total RNA Purification Maxi Kit. One microliter of the 2 mL elution was analyzed on an Agilent® 2100 BioAnalyzer RNA Nano 6000 chip. As it can be seen, high-quality total RNA was isolated with minimal degradation. As well, a large amount of small RNA (< 200 nt) was recovered, indicating that the kit is able to isolate a diversity of RNA species.
Figure 2. Good Amplification and Consistency of Isolation.
Norgen's Total RNA Purification Maxi Kit was used to isolate total RNA from six different overnight samples of E. coli (10 mL samples). In brief, one microgram of the isolated RNA was subjected to a 20 µL reverse transcription using Invitrogen Superscript III. Three microliters of the reverse transcription were used as the template in a 20 µL qPCR reaction using 16S rRNA E. coli specific primers and Bio-Rad iQ mix. As it can be seen, the RNA was detected and successfully amplified in all cases. Furthermore, all samples showed a similar Ct value, indicating consistent isolation.
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Especificaciones del kit
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Capacidad máxima de unión de la columna
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1.5 mg
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Volumen máximo de carga de la columna
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20 mL
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| Tamaño del ARN purificado |
Todos los tamaños, incluido el ARN pequeño
(< 200 nt) |
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Cantidad máxima de material de partida
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Tejido hepático
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100 mg
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Tejido cardiaco
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50 mg
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Tejido renal
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100 mg
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Tejido cerebral
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250 mg
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| Tejido pulmonar |
100 mg
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Sangre entera
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2 – 10 mL*
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| Bacterias | 2.5 x 10 10 células |
| Levadura |
2 x 108 células
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| Hongos |
1 g
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| Tejidos vegetales |
1 g
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| Tiempo para completar 4 purificaciones |
40 minutos
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Rendimiento medio:
Células HeLa (5 x 107 células) E. coli (2.5 x 1010 células) |
~750 μg
~1.5 mg |
*Fara aislar el ARN total de leucocitos purificados
Condiciones de almacenamiento y estabilidad del producto
Todas las soluciones deben mantenerse herméticamente cerradas y conservarse a temperatura ambiente. Este kit es estable durante 2 años a partir de la fecha de envío.