Exosome Depletion
Exosome Depletion
Exosomes: The Essentials
Depletion of exosomes from serum prior to using it as a growth supplement in your culture medium
- Efficient depletion of cow's exosomes from Fetal Bovine Serum or any other serum source
- Deplete exosome-sized vesicles from versatile serum volumes of up to 280 mL
- No protease treatment required
- No time-consuming ultracentrifugation
- No precipitation reagents or overnight incubation required
- Depleted FBS has no detectable cow’s miRNA
- The depleted FBS provides the same cellular growth rates as the standard FBS
- Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
Norgen's FBS Exosome Depletion Kits constitute an all-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen's kits allows for the depletion of different FBS volumes with a maximum volume ranging from 140 mL to 280 mL. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for depletion of your own FBS, as compared to the current ready-to-use exosome-depleted media available on the market.