Figure 1. Accurate Quantity and High Quality Neisseria gonorrhoeae DNA Standard. Two micro-liters of Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard and various serial dilutions (blue curves) were used in a 20 µL Neisseria gonorrhoeae-specific PCR reaction using Norgen’s 2X PCR Mastermix (#28007), supplemented with SYBR Green I. The real-time PCR reactions were performed on a Bio-Rad iCycler iQTM Real-Time PCR System. No template controls with water were also performed (yellow curves). Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard and various serial dilutions showed consistent amplification down to as little as 40 copies per reaction.