Figure 1. Accurate Quantity and High Quality Staphylococcus aureus DNA Standard. Two micro-liters of Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard and various serial dilutions (blue curves) were used in a 20 µL Staphylococcus aureus-specific PCR reaction using Norgen’s 2X PCR Master Mix (#28007), supplemented with SYBR Green I. The real-time PCR reactions were performed on a Bio-Rad CFXReal-Time PCR System. No template controls with water were also performed (yellow curves). Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard and various serial dilutions showed consistent amplification down to fewer than 40 copies per reaction.