Figure 2. Comparison of Saliva DNA Isolation Methods (Column vs. Magnetic Bead System). DNA was isolated from 0.5 mL of preserved saliva samples collected from 6 different donors (A to F) using Norgen's Saliva DNA Isolation Kit (Magnetic Bead System). As a control, DNA was isolated from one preserved saliva sample using Norgen's Saliva DNA Isolation Kit (column format, Cat. RU45400) . All DNA purified using Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) [A to F] showed a comparable concentration to DNA isolated using Norgen’s Saliva DNA Isolation Kit (column format, Cat. RU45400), indicating the consistent and robust performance of the Saliva DNA Isolation Kit (Magnetic Bead System).

Figure 3. High Quality DNA Confirmed by Real-time PCR. DNA was isolated from 0.5 mL of preserved saliva samples collected from 6 different donors (A to F) using Norgen's Saliva DNA Isolation Kit (Magnetic Bead System). As a control, DNA was isolated from one preserved saliva sample using Norgen's Saliva DNA Isolation Kit (column format, Cat. RU45400). Saliva DNA quality was confirmed by Real-time PCR using 8 µL of saliva DNA (total PCR reaction volume was 20 µL) to detect GAPDH. GAPDH was detected from all the saliva DNA isolated using the Magnetic Bead System (blue lines), similar to Norgen’s column based method (Saliva DNA Isolation Kit; Cat. RU45400) (red line). The successful GAPDH detection without PCR inhibition indicates the excellent saliva DNA quality from Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System).

Figure 4. Compatibility Test with a Competitor’s Saliva Collection Device. Identical saliva samples were equally divided into Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000) and Competitor D’s saliva collection device. DNA was then isolated from 0.5 mL of preserved saliva using Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System). For evaluation, 10 µL from each 75 µL of elution were run on 1X TAE 1.2% agarose gel. For the real-time PCR, 8 µL of DNA elution was add to total 20 µL of PCR reaction to detect GAPDH (Blue: Norgen, Red: Competitor D). Marker = Norgen’s HighRanger DNA Ladder. The results show that Norgen's kit was able to isolate high yields of pure DNA from both collection devices, indicating the robust performance of the kit.

Figure 5. Resolution of DNA Isolated from Preserved Saliva Samples from Different Donors. DNA was isolated from 0.5 mL of preserved saliva samples collected from 8 different healthy donors using Norgen's Saliva DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL of each 50 µL elution was run on a 1X TAE 1.2% agarose gel. Marker = Norgen's HighRanger DNA Ladder (Cat. 11900).

Figure 6. High Quality DNA Confirmed by Real-time PCR. DNA was isolated from 0.5 mL of preserved saliva samples collected using Norgen's Saliva DNA Isolation 96-Well Kit (Magnetic Bead System). Saliva DNA quality was confirmed by Real-time PCR using 2 µL of saliva DNA (total PCR reaction volume was 20 µL) to detect GAPDH. GAPDH was detected from all the saliva DNA isolated using the Saliva DNA Isolation 96-Well Kit (Magnetic Bead System). The successful GAPDH detection without PCR inhibition indicates the excellent saliva DNA quality from Norgen’s Saliva DNA Isolation 96-Well Kit (Magnetic Bead System).