Name: NGS Library Quantification Kit (for Small RNA-Seq)
Brand: Norgen Biotek Corp.
SKU: 61600
Price: 115.000000 USD
Availability: InStock

Supporting Data

Figure 1. A representative qPCR baseline graph showing the successful amplification of Quantified NGS Library Standards (Green) with a range from 20 pM to 2 fM, using Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) (Panel A). Duplicate amplification of a sample Small RNA-Seq library (at 1:10,000 dilution) was performed (Orange). The derived library concentration was 9.41 nM. Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) is of good quality as shown with the high PCR efficiency and correlation in the standard curve (Panel B) with low background signals (No Template Control – NTC as Yellow in Panel A).

Figure 2. Accurate standard curve quantification. Norgen's NGS Library Quantification Kit (for Small RNA-Seq) was compared head-to-head with two competitor's NGS Library Quantification Kits on a Bio-Rad CFX96 Real-Time PCR system. Norgen's kit showed both high correlation and PCR efficiency when compared to the competitors' kits.

Storage Conditions
Upon receipt, store Norgen's NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Component	Cat. 61600 (100 reactions)
2X Real-Time PCR Master Mix	1 mL
NGS Library Quantification Primer Set Mix	600 µL
Quantified NGS Library Standards (for Small-RNA Seq)*	5 x 80 µL Standards
Product Insert	1

* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM

Documentation

PROTOCOLS

(61600) NGS Library Quantification Kit (for Small RNA-Seq) - Protocol (100 rxns)

NGS Library Quantification Kit (for Small RNA-Seq)